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Reduction of nitroblue tetrazolium to formazan by folic acid

  • Cesare Achilli EMAIL logo , Stefania Grandi , Annarita Ciana , Cesare Balduini and Giampaolo Minetti
Published/Copyright: January 28, 2014
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Chemical Papers
From the journal Chemical Papers Volume 68 Issue 5

Abstract

The reduction of nitroblue tetrazolium (NBT) to formazan by folic acid, N-(4-aminobenzoyl) glutamic acid, and other amino acids was studied in this paper. The reduction involves only one of the two tetrazolium rings of NBT. The reaction is considerably more rapid with folic acid and N-(4-aminobenzoyl) glutamic acid than with the other amino acids under study. The electron donor moiety appears to be the carboxylic acid in the alpha position. N-ethyl-N′(3-dimethylaminopropyl) carbodiimide notably increases the rate of the reaction and promotes the reduction of both tetrazolium rings.

Keywords: nitroblue tetrazolium; formazan; carbodiimide; folic acid; amino acids

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Published Online: 2014-1-28
Published in Print: 2014-5-1

© 2013 Institute of Chemistry, Slovak Academy of Sciences

Articles in the same Issue

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  2. Catalysis and reaction mechanisms of N-formylation of amines using Fe(III)-exchanged sepiolite
  3. Effect of support on activity of palladium catalysts in nitrobenzene hydrogenation
  4. Biphasic recognition chiral extraction — novel way of separating pantoprazole enantiomers
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  7. Mercury(II) complexes of new bidentate phosphorus ylides: synthesis, spectra and crystal structures
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  11. Synthesis of palladium-bidentate complex and its application in Sonogashira and Suzuki coupling reactions
  12. Reduction of nitroblue tetrazolium to formazan by folic acid
  13. Michael addition of phenylacetonitrile to the acrylonitrile group leading to diphenylpentanedinitrile. Structural data and theoretical calculations
  14. Efficient hydrolysis of glucose-1-phosphate catalyzed by metallomicelles with histidine residue
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https://doi.org/10.2478/s11696-013-0495-5
Keywords for this article
nitroblue tetrazolium; formazan; carbodiimide; folic acid; amino acids

Articles in the same Issue

  1. A spectrophotometric method for plant pigments determination and herbs classification
  2. Catalysis and reaction mechanisms of N-formylation of amines using Fe(III)-exchanged sepiolite
  3. Effect of support on activity of palladium catalysts in nitrobenzene hydrogenation
  4. Biphasic recognition chiral extraction — novel way of separating pantoprazole enantiomers
  5. Effect of the preparation route on the structure and microstructure of LaCoO3
  6. Synthesis, characterisation, and antioxidant study of Cr(III)-rutin complex
  7. Mercury(II) complexes of new bidentate phosphorus ylides: synthesis, spectra and crystal structures
  8. Synthesis and properties of CaAl-layered double hydroxides of hydrocalumite-type
  9. MgZnAl hydrotalcite-like compounds preparation by a green method: effect of zinc content
  10. Carbon nanotube-layered double hydroxide nanocomposites
  11. Synthesis of palladium-bidentate complex and its application in Sonogashira and Suzuki coupling reactions
  12. Reduction of nitroblue tetrazolium to formazan by folic acid
  13. Michael addition of phenylacetonitrile to the acrylonitrile group leading to diphenylpentanedinitrile. Structural data and theoretical calculations
  14. Efficient hydrolysis of glucose-1-phosphate catalyzed by metallomicelles with histidine residue
  15. Synthesis of [Re2Cl4(O)2(µ-O)(3,5-lut)4] and investigation of its structure via X-ray and spectroscopic measurements and DFT calculations
  16. QSAR modeling of aromatase inhibition by flavonoids using machine learning approaches
  17. Influence of freezing on physicochemical forms of natural and technogenic radionuclides in Chernozem soil
  18. “Green synthesis” of benzothiazepine library of indeno analogues and their in vitro antimicrobial activity
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