Abstract
An analytical multiclass, multi-residue method for the determination of antibiotics in aquaculture products was developed and validated. A fast, cheap, and straightforward extraction procedure followed by liquid chromatography-tandem mass spectrometry analysis was proposed. This method covers 32 antibiotics of different classes, which are frequently used in aquaculture. Three different extraction procedures were compared, and the extraction with acetonitrile (0.1 vol. % formic acid) showed the best results. The selected extraction procedure was validated at four different fortification levels (10 μg kg−1, 25 μg kg−1, 50 μg kg−1, and 100 μg kg−1). Recoveries of the tested antibiotics ranged from 70 % to 120 %, with the relative standard deviation (RSD) of triplicates lower than 20 %. The limits of quantification (LOQ) ranged from 0.062 μg kg−1 to 4.6 μg kg−1, allowing for the analysis of trace levels of these antibiotics in aquaculture products. The method was applied to the analysis of selected antibiotics in fish and shrimp meat available in the Czech market.
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© 2013 Institute of Chemistry, Slovak Academy of Sciences
Artikel in diesem Heft
- Immobilisation of Clostridium spp. for production of solvents and organic acids
- A multi-analytical approach to amber characterisation
- Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography
- Simultaneous determination of 32 antibiotics in aquaculture products using LC-MS/MS
- In vitro and in silico inhibition of angiotensin-converting enzyme by carbohydrates and cyclitols
- 5-Bromo- and 3,5-dibromo-2-hydroxy-N-phenylbenzamides — inhibitors of photosynthesis
- Biotransformation of iminodiacetonitrile to iminodiacetic acid by Alcaligenes faecalis cells immobilized in ACA-membrane liquid-core capsules
- Kinetics of metribuzin degradation by colloidal manganese dioxide in absence and presence of surfactants
- Asymmetric deformation of bubble shape: cause or effect of vortex-shedding?
- Equilibrium of chiral extraction of 4-nitro-d,l-phenylalanine with BINAP metal complexes
- Influence of superplasticizers on the course of Portland cement hydration
- Effect of valence of copper on adsorption of dimethyl sulfide from liquid hydrocarbon streams on activated bentonite
- Kinetics of tartrazine photodegradation by UV/H2O2 in aqueous solution
- Effect of exopolymeric substances on the kinetics of sorption and desorption of trivalent chromium in soil
- Thermal stability, antioxidant activity, and photo-oxidation of natural polyphenols
- Kinetics of chloride substitution in [Pt(bpma)Cl]+ and [Pt(gly-met-S,N,N)Cl] complexes by thiourea, nitrites, and iodides
- Synthesis of a photoactive gemini surfactant and its use in AGET ATRP miniemulsion polymerisation and UV curing
- Theoretical investigation on the reaction of HS+ with CH3NH2
Artikel in diesem Heft
- Immobilisation of Clostridium spp. for production of solvents and organic acids
- A multi-analytical approach to amber characterisation
- Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography
- Simultaneous determination of 32 antibiotics in aquaculture products using LC-MS/MS
- In vitro and in silico inhibition of angiotensin-converting enzyme by carbohydrates and cyclitols
- 5-Bromo- and 3,5-dibromo-2-hydroxy-N-phenylbenzamides — inhibitors of photosynthesis
- Biotransformation of iminodiacetonitrile to iminodiacetic acid by Alcaligenes faecalis cells immobilized in ACA-membrane liquid-core capsules
- Kinetics of metribuzin degradation by colloidal manganese dioxide in absence and presence of surfactants
- Asymmetric deformation of bubble shape: cause or effect of vortex-shedding?
- Equilibrium of chiral extraction of 4-nitro-d,l-phenylalanine with BINAP metal complexes
- Influence of superplasticizers on the course of Portland cement hydration
- Effect of valence of copper on adsorption of dimethyl sulfide from liquid hydrocarbon streams on activated bentonite
- Kinetics of tartrazine photodegradation by UV/H2O2 in aqueous solution
- Effect of exopolymeric substances on the kinetics of sorption and desorption of trivalent chromium in soil
- Thermal stability, antioxidant activity, and photo-oxidation of natural polyphenols
- Kinetics of chloride substitution in [Pt(bpma)Cl]+ and [Pt(gly-met-S,N,N)Cl] complexes by thiourea, nitrites, and iodides
- Synthesis of a photoactive gemini surfactant and its use in AGET ATRP miniemulsion polymerisation and UV curing
- Theoretical investigation on the reaction of HS+ with CH3NH2