Startseite Biotransformation of iminodiacetonitrile to iminodiacetic acid by Alcaligenes faecalis cells immobilized in ACA-membrane liquid-core capsules
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Biotransformation of iminodiacetonitrile to iminodiacetic acid by Alcaligenes faecalis cells immobilized in ACA-membrane liquid-core capsules

  • Jin-Feng Zhang EMAIL logo , Zhi-Qiang Liu , Xin-Hong Zhang und Yu-Guo Zheng
Veröffentlicht/Copyright: 17. September 2013
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Chemical Papers
Aus der Zeitschrift Chemical Papers Band 68 Heft 1

Abstract

Biotransformation of iminodiacetonitrile (IDAN) to iminodiacetic acid (IDA) was investigated with a newly isolated Alcaligenes faecalis ZJUTBX11 strain showing nitrilase activity in the immobilized form. To reduce the mass transfer resistance and to increase the toleration ability of the microorganisms to the toxic substrate as well as to enhance their ability to be reused, encapsulation of the whole cells in alginate-chitosan-alginate (ACA) membrane liquid-core capsules was attempted in the present study. The optimal pH and temperature for nitrilase activity of encapsulated A. faecalis ZJUTBX11 cells were 7.5°C and 35°C, respectively, which is consistent with free cells. Based on the Michaelis-Menten model, kinetic parameters of the conversion reaction with IDAN as the substrate were: K m = (17.6 ± 0.3) mmol L−1 and V max = (97.6 ± 1.2) μmol min−1 g−1 of dry cell mass for encapsulated cells and (16.8 ± 0.4) mmol L−1 and (108.0 ± 2.7) μmol min−1 g−1 of dry cell mass for free cells, respectively. After being recycled ten times, the whole cells encapsulated in ACA capsules still retained 90 % of the initial nitrilase activity while only 35 % were retained by free cells. Lab scale production of IDA using encapsulated cells in a bubble column reactor and a packed bed reactor were performed respectively.

Keywords: biotransformation; iminodiacetic acid; microencapsulation; Alcaligenes faecalis; nitrilase; bubble column reactor

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Published Online: 2013-9-17
Published in Print: 2014-1-1

© 2013 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.2478/s11696-013-0423-8
Schlagwörter für diesen Artikel
biotransformation; iminodiacetic acid; microencapsulation; Alcaligenes faecalis; nitrilase; bubble column reactor

Artikel in diesem Heft

  1. Immobilisation of Clostridium spp. for production of solvents and organic acids
  2. A multi-analytical approach to amber characterisation
  3. Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography
  4. Simultaneous determination of 32 antibiotics in aquaculture products using LC-MS/MS
  5. In vitro and in silico inhibition of angiotensin-converting enzyme by carbohydrates and cyclitols
  6. 5-Bromo- and 3,5-dibromo-2-hydroxy-N-phenylbenzamides — inhibitors of photosynthesis
  7. Biotransformation of iminodiacetonitrile to iminodiacetic acid by Alcaligenes faecalis cells immobilized in ACA-membrane liquid-core capsules
  8. Kinetics of metribuzin degradation by colloidal manganese dioxide in absence and presence of surfactants
  9. Asymmetric deformation of bubble shape: cause or effect of vortex-shedding?
  10. Equilibrium of chiral extraction of 4-nitro-d,l-phenylalanine with BINAP metal complexes
  11. Influence of superplasticizers on the course of Portland cement hydration
  12. Effect of valence of copper on adsorption of dimethyl sulfide from liquid hydrocarbon streams on activated bentonite
  13. Kinetics of tartrazine photodegradation by UV/H2O2 in aqueous solution
  14. Effect of exopolymeric substances on the kinetics of sorption and desorption of trivalent chromium in soil
  15. Thermal stability, antioxidant activity, and photo-oxidation of natural polyphenols
  16. Kinetics of chloride substitution in [Pt(bpma)Cl]+ and [Pt(gly-met-S,N,N)Cl] complexes by thiourea, nitrites, and iodides
  17. Synthesis of a photoactive gemini surfactant and its use in AGET ATRP miniemulsion polymerisation and UV curing
  18. Theoretical investigation on the reaction of HS+ with CH3NH2
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