Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography
-
Martin Franc
,
Jiří Vojta
Abstract
Fused silica capillary columns of the internal diameter of 320 μm were packed with the Nucleosil C18 stationary phase of 5 μm using the slurry packing method. The time of the bed compaction phase, packing pressure, and the use of ultrasound varied to study their influence on the column performance. Van Deemter curves were measured and separation impedance values were calculated in order to assess both separation efficiency and kinetic performance of the columns. Selected columns were tested again after nine months to evaluate the stability of their beds. Separation efficiencies of all columns were similar, but a major difference, caused by the use of ultrasound, was observed in the bed stability. Columns sonicated for 25 minutes during the bed compaction phase exhibited unchanged performance in the course of several months, while the performance of non-sonicated columns decreased.
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© 2013 Institute of Chemistry, Slovak Academy of Sciences
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- Immobilisation of Clostridium spp. for production of solvents and organic acids
- A multi-analytical approach to amber characterisation
- Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography
- Simultaneous determination of 32 antibiotics in aquaculture products using LC-MS/MS
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- 5-Bromo- and 3,5-dibromo-2-hydroxy-N-phenylbenzamides — inhibitors of photosynthesis
- Biotransformation of iminodiacetonitrile to iminodiacetic acid by Alcaligenes faecalis cells immobilized in ACA-membrane liquid-core capsules
- Kinetics of metribuzin degradation by colloidal manganese dioxide in absence and presence of surfactants
- Asymmetric deformation of bubble shape: cause or effect of vortex-shedding?
- Equilibrium of chiral extraction of 4-nitro-d,l-phenylalanine with BINAP metal complexes
- Influence of superplasticizers on the course of Portland cement hydration
- Effect of valence of copper on adsorption of dimethyl sulfide from liquid hydrocarbon streams on activated bentonite
- Kinetics of tartrazine photodegradation by UV/H2O2 in aqueous solution
- Effect of exopolymeric substances on the kinetics of sorption and desorption of trivalent chromium in soil
- Thermal stability, antioxidant activity, and photo-oxidation of natural polyphenols
- Kinetics of chloride substitution in [Pt(bpma)Cl]+ and [Pt(gly-met-S,N,N)Cl] complexes by thiourea, nitrites, and iodides
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