Immobilization of α-amylase from Aspergillus fumigatus using adsorption method onto zeolite
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Yandri Yandri
,
Hendri Ropingi
Abstract
The stability of enzymes which play an important role as biocatalysts in many industrial processes is a persistent challenge with significant impact on production costs. In this study, improvement of the stability of α-amylase obtained from Aspergillus fumigatus was attempted by immobilizing the enzyme onto zeolite using adsorption method. For purification, the isolated enzyme was subsequently subjected to centrifugation, fractionation, and dialysis. The native enzyme was found to have an optimum temperature of 50 °C, while the immobilized enzyme, the optimum temperature of 60 °C was found. The immobilized enzyme was found to have the K M value of 11.685 ± 0.183 mg mL−1 substrate and V max of 1.406 ± 0.049 μmol mL−1 min−1, while for the native enzyme, the K M value of 3.478 ± 0.271 mg mL−1 substrate and the V max of 2.211 ± 0.096 μmol mL−1 min−1 were obtained. Furthermore, the immobilized enzyme displays the ΔGi of 106.76 ± 0.00 kJ mol−1 and t ½ of 90.40 ± 0.00 min, while the native enzyme, the values obtained are ΔGi of 104.35 ± 1.09 kJ mol−1 and t½ of 38.75 ± 1.53 min. As can be seen, the t ½ of immobilized enzyme is 2.38 times longer than that of native enzyme, justifying a very significant stability enhancement of the enzyme as a result of. Another important finding is that the immobilized α-amylase enzyme was able to retain its activity as high as 13.80 ± 1.19% activity after five cycles, indicating its potential for industrial use.
Funding source: Ministry of Education, Culture, Research and Technology
Award Identifier / Grant number: 114/E5/PG.02.00/PT/2022
Award Identifier / Grant number: 2147/UN26.21/PN /2022
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Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: The author would like to thank the Ministry of Education, Culture, Research and Technology, for funding support in the form of Basic Research, fiscal year 2022 with contract number 114/E5/PG.02.00/PT/2022 on 10th May 2022 and 2147/UN26.21/PN /2022 on 13th May, 2022.
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Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
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© 2023 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Reviews
- Topology and applications of 2D Dirac and semi-Dirac materials
- Landscape ecological modeling to identify ecologically significant regions in Tumkur district, Karnataka
- Surfactants-surface active agents behind sustainable living
- Ecological footprint of poultry production and effect of environment on poultry genes
- Fluoride in water, health implications and plant-based remediation strategies
- Poultry nutrition
- Synthesis of N-containing heterocycles in water
- Inorganic nanoparticles promoted synthesis of heterocycles
- The role of analytical chemistry in poultry science
- Antibiotics in avian care and husbandry-status and alternative antimicrobials
- Removal of heavy metals from wastewater using synthetic chelating agents
- Azadirachtin in the aquatic environment: Fate and effects on non-target fauna
- Intensification of bioprocesses with filamentous microorganisms
- The science of genetically modified poultry
- Emerging in ovo technologies in poultry production and the re-discovered chicken model in preclinical research
- The Cambridge structural database (CSD): important resources for teaching concepts in structural chemistry and intermolecular interactions
- Microbial production of lactic acid using organic wastes as low-cost substrates
- Oxalic acid: recent developments for cost-effective microbial production
- Immobilization of α-amylase from Aspergillus fumigatus using adsorption method onto zeolite
- A comparative assessment of potentially harmful metals in the Lagos Lagoon and Ogun river catchment
- Formulation of a herbal topical cream against Tinea capitis using flavonoids glycosides from Dicerocaryum senecioides and Diospyros mespiliformis
- Biodegradable polymers – research and applications
- Adsorption of trichloroacetic acid from drinking water using polyethylene terephthalate waste carbon and periwinkle shells–based chitosan
- The vital use of isocyanide-based multicomponent reactions (MCR) in chemical synthesis
- Pine bark crosslinked to cyclodextrin for the adsorption of 2-nitrophenol from an aqueous solution
- Computational study of propene selectivity and yield in the dehydrogenation of propane via process simulation approach
- A mini review on the prospects of Fagara zanthoxyloides extract based composites: a remedy for COVID-19 and associated replica?
- Physicochemical assessment and insilico studies on the interaction of 5-HT2c receptor with herbal medication bioactive compounds used in the treatment of premature ejaculation
- Horse chestnut thermoplastic starch nanocomposite films reinforced with nanocellulose
- Rice thermoplastic starch nanocomposite films reinforced with nanocellulose
Artikel in diesem Heft
- Frontmatter
- Reviews
- Topology and applications of 2D Dirac and semi-Dirac materials
- Landscape ecological modeling to identify ecologically significant regions in Tumkur district, Karnataka
- Surfactants-surface active agents behind sustainable living
- Ecological footprint of poultry production and effect of environment on poultry genes
- Fluoride in water, health implications and plant-based remediation strategies
- Poultry nutrition
- Synthesis of N-containing heterocycles in water
- Inorganic nanoparticles promoted synthesis of heterocycles
- The role of analytical chemistry in poultry science
- Antibiotics in avian care and husbandry-status and alternative antimicrobials
- Removal of heavy metals from wastewater using synthetic chelating agents
- Azadirachtin in the aquatic environment: Fate and effects on non-target fauna
- Intensification of bioprocesses with filamentous microorganisms
- The science of genetically modified poultry
- Emerging in ovo technologies in poultry production and the re-discovered chicken model in preclinical research
- The Cambridge structural database (CSD): important resources for teaching concepts in structural chemistry and intermolecular interactions
- Microbial production of lactic acid using organic wastes as low-cost substrates
- Oxalic acid: recent developments for cost-effective microbial production
- Immobilization of α-amylase from Aspergillus fumigatus using adsorption method onto zeolite
- A comparative assessment of potentially harmful metals in the Lagos Lagoon and Ogun river catchment
- Formulation of a herbal topical cream against Tinea capitis using flavonoids glycosides from Dicerocaryum senecioides and Diospyros mespiliformis
- Biodegradable polymers – research and applications
- Adsorption of trichloroacetic acid from drinking water using polyethylene terephthalate waste carbon and periwinkle shells–based chitosan
- The vital use of isocyanide-based multicomponent reactions (MCR) in chemical synthesis
- Pine bark crosslinked to cyclodextrin for the adsorption of 2-nitrophenol from an aqueous solution
- Computational study of propene selectivity and yield in the dehydrogenation of propane via process simulation approach
- A mini review on the prospects of Fagara zanthoxyloides extract based composites: a remedy for COVID-19 and associated replica?
- Physicochemical assessment and insilico studies on the interaction of 5-HT2c receptor with herbal medication bioactive compounds used in the treatment of premature ejaculation
- Horse chestnut thermoplastic starch nanocomposite films reinforced with nanocellulose
- Rice thermoplastic starch nanocomposite films reinforced with nanocellulose
