Effect of Annona squamosa leaf extract on human promyelocytic leukemia cells subjected to oxidative stress
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Lowell L. Dilworth
,
Dewayne K. Stennett
Abstract
Objectives
Annona squamosa has beneficial properties. However, its cytotoxicity and antioxidative effects on human promyelocytic leukemia cells (HL60) deserve investigation. Therefore, the efficacy of its crude extracts in offsetting damage in HL60 cells subjected to oxidative stress was studied.
Methods
Crude extracts at different concentrations were incubated with HL60 cells. The beneficial properties of the plant extract against oxidative damage were evaluated post-induction of oxidative stress utilizing hydrogen peroxide.
Results
Extracts at concentrations 600 and 800 μg/mL were most effective at increasing the viability of damaged cells compared to the control group after 48 h of incubation. Significant increases in lipid peroxidation were observed in exposed cells treated with 600 μg/mL extract after 72 h of incubation. Superoxide dismutase (SOD) and catalase activities significantly increased in exposed cells after 24 h of incubation at all extract concentrations. Exposed cells treated with 600 and 1,000 μg/dL of the extract showed significantly increased catalase activity after 48 h, and a similar profile was maintained after 72 h of exposure. SOD activity in exposed cells remained significantly increased at all treatment concentrations after 48 and 72 h of incubation. Treatment with 400, 600, and 800 μg/mL of the extract resulted in significantly increased reduced glutathione levels compared to the other groups after 24 and 72 h of incubation. However, after 48 h of incubation, significant increases were noted in glutathione levels in exposed cells incubated with either 400, 800, or 1,000 μg/mL extract.
Conclusions
The findings suggest that A. squamosa might effectively protect against oxidative damage in a time and extract concentration-dependent manner.
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Research funding: None declared.
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Author contributions: All the authors have contributed and accepted responsibility for the entire content of this manuscript and have also approved submission.
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Competing interests: Authors declare no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: The local Institutional Review Board deemed the study exempt from review.
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© 2023 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Reviews
- The effect of lavender on mothers sleep quality in the postpartum period: a systematic review and meta-analysis
- The effect of Salvia officinalis on blood glycemic indexes and blood lipid profile in diabetic patients: a systematic review and meta-analysis
- Neuroprotective potential of Celastrus paniculatus seeds against common neurological ailments: a narrative review
- Intuitive and mindful eating to improve physiological health parameters: a short narrative review of intervention studies
- Research Articles
- Vitamin C supplementation modulates crude oil contaminated water induced gravid uterine impaired contractile mechanism and foetal outcomes in Wistar rats
- Acute and sub acute toxicity of Amirdha Gandhi Kukkil Vallathy an effective Siddha herbo mineral formulation used in the treatment of rheumatoid arthritis in wistar albino rat model
- Anti-hyperplastic effects of the Dacryodes edulis (Burseraceae) leaves aqueous extract on tamoxifen-induced endometrium hyperplasia on Wistar rat
- Vitamin A and vitamin D3 protect the visual apparatus during the development of dopamine-2 receptor knockout mouse model of Parkinsonism
- Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats
- Chemical composition, antioxidant, and enzyme inhibitory properties of Rauwolfia vomitoria extract
- Intra-stromal injection of honey-treated keratocytes as a cell-based therapy for experimental corneal laceration
- Effect of Annona squamosa leaf extract on human promyelocytic leukemia cells subjected to oxidative stress
- Cinnamic acids as promising bioactive compounds for cancer therapy by targeting MAPK3: a computational simulation study
- Cowanin induces apoptosis in breast cancer cells via Bcl-2 signaling pathway
- Arq Ajīb – a wonder Unani formulation for inhibiting SARS-CoV-2 spike glycoprotein and main protease – an in silico approach
- Immediate effect of a meditation technique on blood glucose, state anxiety and relaxation in patients with type 2 diabetes: a pilot randomized crossover study
- Effect of moderate intensity resistance training with blood flow restriction on muscle strength and girth in young adults – a randomized control trial
- Short Communication
- Icosapent ethyl (VASCEPA®) as treatment for post-acute sequelae of SARS CoV-2 (PASC) vaccine induced injury and infection
Artikel in diesem Heft
- Frontmatter
- Reviews
- The effect of lavender on mothers sleep quality in the postpartum period: a systematic review and meta-analysis
- The effect of Salvia officinalis on blood glycemic indexes and blood lipid profile in diabetic patients: a systematic review and meta-analysis
- Neuroprotective potential of Celastrus paniculatus seeds against common neurological ailments: a narrative review
- Intuitive and mindful eating to improve physiological health parameters: a short narrative review of intervention studies
- Research Articles
- Vitamin C supplementation modulates crude oil contaminated water induced gravid uterine impaired contractile mechanism and foetal outcomes in Wistar rats
- Acute and sub acute toxicity of Amirdha Gandhi Kukkil Vallathy an effective Siddha herbo mineral formulation used in the treatment of rheumatoid arthritis in wistar albino rat model
- Anti-hyperplastic effects of the Dacryodes edulis (Burseraceae) leaves aqueous extract on tamoxifen-induced endometrium hyperplasia on Wistar rat
- Vitamin A and vitamin D3 protect the visual apparatus during the development of dopamine-2 receptor knockout mouse model of Parkinsonism
- Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats
- Chemical composition, antioxidant, and enzyme inhibitory properties of Rauwolfia vomitoria extract
- Intra-stromal injection of honey-treated keratocytes as a cell-based therapy for experimental corneal laceration
- Effect of Annona squamosa leaf extract on human promyelocytic leukemia cells subjected to oxidative stress
- Cinnamic acids as promising bioactive compounds for cancer therapy by targeting MAPK3: a computational simulation study
- Cowanin induces apoptosis in breast cancer cells via Bcl-2 signaling pathway
- Arq Ajīb – a wonder Unani formulation for inhibiting SARS-CoV-2 spike glycoprotein and main protease – an in silico approach
- Immediate effect of a meditation technique on blood glucose, state anxiety and relaxation in patients with type 2 diabetes: a pilot randomized crossover study
- Effect of moderate intensity resistance training with blood flow restriction on muscle strength and girth in young adults – a randomized control trial
- Short Communication
- Icosapent ethyl (VASCEPA®) as treatment for post-acute sequelae of SARS CoV-2 (PASC) vaccine induced injury and infection
