Cowanin induces apoptosis in breast cancer cells via Bcl-2 signaling pathway

Fatma Sri Wahyuni; Suryati Syafri; Dita Permatasari; Dira Hefni; Dachriyanus Hamidi; Nur Azizah Nomiza; Indri Aulia Rezti

doi:10.1515/jcim-2023-0058

Article

Cowanin induces apoptosis in breast cancer cells via Bcl-2 signaling pathway

Fatma Sri Wahyuni , Suryati Syafri , Dita Permatasari , Dira Hefni , Dachriyanus Hamidi , Nur Azizah Nomiza and Indri Aulia Rezti

Published/Copyright: May 22, 2023

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From the journal Journal of Complementary and Integrative Medicine Volume 20 Issue 3

Abstract

Objectives

This study aimed to investigate the effect of cowanin the mechanism of cowanin toward cell death and BCL-2 protein (antiapoptotic) expression of T47D breast cancer.

Methods

The cell death was evaluated by double staining, namely acridine orange and propidium iodide, and then observed under a fluorescence microscope. Meanwhile, the BCL-2 protein expression was determined by western blotting with measurement of protein area and protein density.

Results

The result found T47D breast cancer cells were viable, apoptosis, and necrosis after treatment with cowanin. The average viable cells, apoptosis, and necrosis percentages were 54.13 %, 45.43 %, and 0.44 %, respectively. Statistical analysis showed cowanin could significantly induce death in T47D breast cancer cells by apoptosis (p<0.05). It was also revealed that cowanin and positive control (doxorubicin) treatment had a significantly decreased protein area and protein density (p<0.05).

Conclusions

It can be concluded that cowanin can induce death in T47D breast cancer cells by apoptosis and affect the expression of Bcl-2 protein in T47D breast cancer cells.

Keywords: cells death; double staining; protein expression; T47D cells; western blot

Corresponding author: Prof. Dr. apt. Fatma Sri Wahyuni, Faculty of Pharmacy, Universitas Andalas, Kampus Limau Manis, Padang, West Sumatra, 25163, Indonesia, E-mail: fatmasriwahyuni@phar.unand.ac.id

Funding source: Research and Community Service Institute, Universitas Andalas, through basic research scheme of professor publication research cluster (PDU-KRP1GB-UNAND) Batch 1 2023

Award Identifier / Grant number: no: T/6/UN16.19/KO-PDU-KRP1GB-Unand/2023

Research funding: This Research was funded by Research and community service Institute, Universitas Andalas, through basic research scheme of professor publication research cluster (PDU-KRP1GB-UNAND) Batch 1 2023 with contracts no T/6/UN16.19/KO-PDU-KRP1GB-Unand/2023.
Author contributions: Fatma Sri Wahyuni: Conceptualization, Supervision, Resources; Suryati: Writing – Original Draft, Writing – Review & Editing; Dira Hefni: Writing – Original Draft, Writing – Review & Editing, Dita Permata sari: Supervision, Methodology; Nur Azizah Nomiza: Methodology, Writing – Original Draft; Indri Aulia Rezti: Methodology, Writing – Original Draft; Dachriyanus: Supervision, Resources, Funding acquisition, Review & Editing.
Competing interests: Authors state no competing of interest.
Informed consent: Not applicable.
Ethical approval: The local Institutional Review Board deemed the study exempt from review.

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Received: 2023-03-02

Accepted: 2023-04-19

Published Online: 2023-05-22

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Articles in the same Issue

https://doi.org/10.1515/jcim-2023-0058

Keywords for this article

cells death; double staining; protein expression; T47D cells; western blot