Chemical composition, antioxidant, and enzyme inhibitory properties of Rauwolfia vomitoria extract

Adeniyi A. Adebayo; Ayokunle O. Ademosun; Ganiyu Oboh

doi:10.1515/jcim-2022-0390

Article

Chemical composition, antioxidant, and enzyme inhibitory properties of Rauwolfia vomitoria extract

Adeniyi A. Adebayo , Ayokunle O. Ademosun and Ganiyu Oboh

Published/Copyright: May 22, 2023

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From the journal Journal of Complementary and Integrative Medicine Volume 20 Issue 3

Abstract

Objectives

Rauwolfia vomitoria is one ethno-botanicals in Nigeria used by traditional health practitioners in managing several human diseases. However, necessary information regarding its effect on enzymes implicated in the development and progression of erectile dysfunction is missing in the literature. Thus, this study investigated the antioxidant property and impact of Rauwolfia vomitoria extract on erectile dysfunction-related enzymes in vitro.

Methods

High performance liquid chromatography was used to identify and quantify Rauwolfia vomitoria’s phenolic components. Then, utilizing common antioxidant assays, the extract’s antioxidant properties were evaluated and finally the effect of the extract on some enzymes (AChE, arginase and ACE) implicated in erectile dysfunction was investigated in vitro.

Results

The results showed that the extract inhibited AChE (IC₅₀=388.72 μg/mL), arginase (IC₅₀=40.06 μg/mL) and ACE (IC₅₀=108.64 μg/mL) activities. In addition, phenolic rich extract of Rauvolfia vomitoria scavenged radicals and chelated Fe²⁺ in concentration dependent manner. Furthermore, rutin, chlorogenic acid, gallic acid, and kaempferol were found in large quantities by HPLC analysis.

Conclusions

Therefore, one of the potential reasons driving Rauwolfia vomitoria’s use in folk medicine for the treatment of erectile dysfunction could be its antioxidant and inhibitory activities on several enzymes linked to erectile dysfunction in vitro.

Keywords: antioxidant; erectile dysfunction; prooxidants; Rauwolfia vomitoria

Corresponding author: Adeniyi A. Adebayo, Chemical Science Department (Biochemistry Unit), Joseph Ayo Babalola University, P.M.B. 5006, Ikeji-Arakeji 230001, Nigeria; and Functional Foods and Nutraceutical Unit, Biochemistry Department, Federal University of Technology, P.M.B. 704, Akure 340001, Nigeria, E-mail: adeniyiabiodun2@gmail.com

Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interest: Authors state no conflict of interest.
Informed consent: Not applicable.
Ethical approval: The local Institutional Review Board deemed the study exempt from review. The use of animal complied with all the relevant national regulation and institutional policies for the care and use of animals. The procedure was approved by the ethical committee, Centre for Research and Development, Federal University of Technology, Akure, Nigeria (FUTA/ETH/21/10).

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Received: 2022-11-14

Accepted: 2023-05-02

Published Online: 2023-05-22

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Articles in the same Issue

https://doi.org/10.1515/jcim-2022-0390

Keywords for this article

antioxidant; erectile dysfunction; prooxidants; Rauwolfia vomitoria