Acute and sub acute toxicity of Amirdha Gandhi Kukkil Vallathy an effective Siddha herbo mineral formulation used in the treatment of rheumatoid arthritis in wistar albino rat model
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Juliet Lawrence
,
Sugin Lal Jabaris S
Abstract
Objectives
Amirdha Gandhi Kukkil Vallathy (AGKV) is a herbo mineral Siddha formulation which has Sulphur, Kukkil, Seendhil and Serankottai as major ingredients and it is indicated for 80 types of Vatham diseases. Uthiravatha suronitham is one among the vatham diseases and it’s clinical symptoms are correlated with Rheumatoid arthritis (RA). Since AGKV is a promising drug for RA, the safety of this drug has been validated by performing the acute and 28 days repeated oral dose toxicity study following the OECD guidelines 423 and 407.
Methods
The acute toxicity study has been performed by administering orally with a single dose of 300 and 2000 mg/kg body weight in rat models and the animals were observed for 14 consecutive days. Gross pathology was observed and animals were sacrificed at the end of the study. In 28 days repeated oral toxicity study, limit test has been carried out with a dose of 1,000 mg/kg body weight.
Results
No significant abnormality has been observed in the body weight, organ weight, biochemical parameters and histopathology studies. It has been revealed that this drug is safe upto 2000 mg/kg body weight in single dose study and 1,000 mg is a safer dose in the 28 days repeated oral toxicity study.
Conclusions
The results of acute and 28 days repeated oral toxicity studies revealed no adverse effects in animals and hence this drug AGKV is safe and can be administered in human.
Acknowledgments
Authors thank all the staff of Department of Pharmacology and Department of Bio chemistry for supporting the research during the whole study as per protocol.
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Research funding: None declared (Part time PhD–study).
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Author contributions: LJ – research work, conceptualized, drafted the manuscript, RB-assisting the study SSL – reviewed the pharmacological part SSK-assisted in writing the manuscript, KK, NK and CJC-Supervised the study, RG-analysis of blood investigations and report. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: The Institutional Animal Ethical Committee approved the study. The Doctoral Advisory Committee supervised the study.
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Artikel in diesem Heft
- Frontmatter
- Reviews
- The effect of lavender on mothers sleep quality in the postpartum period: a systematic review and meta-analysis
- The effect of Salvia officinalis on blood glycemic indexes and blood lipid profile in diabetic patients: a systematic review and meta-analysis
- Neuroprotective potential of Celastrus paniculatus seeds against common neurological ailments: a narrative review
- Intuitive and mindful eating to improve physiological health parameters: a short narrative review of intervention studies
- Research Articles
- Vitamin C supplementation modulates crude oil contaminated water induced gravid uterine impaired contractile mechanism and foetal outcomes in Wistar rats
- Acute and sub acute toxicity of Amirdha Gandhi Kukkil Vallathy an effective Siddha herbo mineral formulation used in the treatment of rheumatoid arthritis in wistar albino rat model
- Anti-hyperplastic effects of the Dacryodes edulis (Burseraceae) leaves aqueous extract on tamoxifen-induced endometrium hyperplasia on Wistar rat
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- Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats
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- Intra-stromal injection of honey-treated keratocytes as a cell-based therapy for experimental corneal laceration
- Effect of Annona squamosa leaf extract on human promyelocytic leukemia cells subjected to oxidative stress
- Cinnamic acids as promising bioactive compounds for cancer therapy by targeting MAPK3: a computational simulation study
- Cowanin induces apoptosis in breast cancer cells via Bcl-2 signaling pathway
- Arq Ajīb – a wonder Unani formulation for inhibiting SARS-CoV-2 spike glycoprotein and main protease – an in silico approach
- Immediate effect of a meditation technique on blood glucose, state anxiety and relaxation in patients with type 2 diabetes: a pilot randomized crossover study
- Effect of moderate intensity resistance training with blood flow restriction on muscle strength and girth in young adults – a randomized control trial
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Artikel in diesem Heft
- Frontmatter
- Reviews
- The effect of lavender on mothers sleep quality in the postpartum period: a systematic review and meta-analysis
- The effect of Salvia officinalis on blood glycemic indexes and blood lipid profile in diabetic patients: a systematic review and meta-analysis
- Neuroprotective potential of Celastrus paniculatus seeds against common neurological ailments: a narrative review
- Intuitive and mindful eating to improve physiological health parameters: a short narrative review of intervention studies
- Research Articles
- Vitamin C supplementation modulates crude oil contaminated water induced gravid uterine impaired contractile mechanism and foetal outcomes in Wistar rats
- Acute and sub acute toxicity of Amirdha Gandhi Kukkil Vallathy an effective Siddha herbo mineral formulation used in the treatment of rheumatoid arthritis in wistar albino rat model
- Anti-hyperplastic effects of the Dacryodes edulis (Burseraceae) leaves aqueous extract on tamoxifen-induced endometrium hyperplasia on Wistar rat
- Vitamin A and vitamin D3 protect the visual apparatus during the development of dopamine-2 receptor knockout mouse model of Parkinsonism
- Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats
- Chemical composition, antioxidant, and enzyme inhibitory properties of Rauwolfia vomitoria extract
- Intra-stromal injection of honey-treated keratocytes as a cell-based therapy for experimental corneal laceration
- Effect of Annona squamosa leaf extract on human promyelocytic leukemia cells subjected to oxidative stress
- Cinnamic acids as promising bioactive compounds for cancer therapy by targeting MAPK3: a computational simulation study
- Cowanin induces apoptosis in breast cancer cells via Bcl-2 signaling pathway
- Arq Ajīb – a wonder Unani formulation for inhibiting SARS-CoV-2 spike glycoprotein and main protease – an in silico approach
- Immediate effect of a meditation technique on blood glucose, state anxiety and relaxation in patients with type 2 diabetes: a pilot randomized crossover study
- Effect of moderate intensity resistance training with blood flow restriction on muscle strength and girth in young adults – a randomized control trial
- Short Communication
- Icosapent ethyl (VASCEPA®) as treatment for post-acute sequelae of SARS CoV-2 (PASC) vaccine induced injury and infection
