Cytoprotective and antisecretory properties of methanolic extract of Distemonanthus benthamianus (Caesalpiniaceae) stem bark on acute gastric ulcer in rats

Vanessa Mba Matah Marthe; Gilbert Ateufack; Marius Mbiantcha; William Yousseu Nana; Albert Donatien Atsamo; Flore Carine Adjouzem; Flore Stephanie Djuichou Nguemnang; Gonzal Eric Tsafack; Herve Tchoumbou Tadjoua; Joseph Emakoua

doi:10.1515/jcim-2019-0216

Artikel

Cytoprotective and antisecretory properties of methanolic extract of Distemonanthus benthamianus (Caesalpiniaceae) stem bark on acute gastric ulcer in rats

Vanessa Mba Matah Marthe , Gilbert Ateufack , Marius Mbiantcha , William Yousseu Nana , Albert Donatien Atsamo , Flore Carine Adjouzem , Flore Stephanie Djuichou Nguemnang , Gonzal Eric Tsafack , Herve Tchoumbou Tadjoua und Joseph Emakoua

Veröffentlicht/Copyright: 24. Juli 2020

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Aus der Zeitschrift Journal of Complementary and Integrative Medicine Band 18 Heft 1

Abstract

Objectives

In African traditional medicine, Distemonanthus benthamianus (Caesalpiniaceae) is used to treat many diseases including gastric ulcers. We evaluated in this study, the cytoprotective and antisecretory properties of the methanolic extract of the stem bark of this plant using different technics of gastric lesion induction.

Methods

Cytoprotective and antisecretory activity of the methanolic extract of D. benthamianus stem bark was evolved through six methods of gastric lesion induction in experimental Wistar male rats (150–200 g): (1) gastric lesions induced by HCl/ethanol, (2) gastric lesions induced by Indomethacin- HCl/ethanol, (3) gastric lesion induced by Indomethacin, (4) gastric lesions induced by Pylorus ligation, (5) gastric lesions induced by histamine-Pylorus ligation, (6) gastric lesions induced by carbachol-Pylorus ligation. Mucus and gastric mucosal ulceration were evaluated. pH, gastric volume, and acidity were quantified in all pylorus ligation induction technics. Nitric oxide (NO) level was determined in indomethacin induced gastric ulcers.

Results

At different doses (125, 250 and 500 mg/kg), extract reduced significantly the ulcer index. In all models used, that is 100.00% with HCl/ethanol; 100.00% with HCl/ethanol/indomethacin; 95.70% with Indomethacin; 74.79% with pylorus ligation, 95.94% histamine-Pylorus ligation, 99.54% carbachol-Pylorus ligation at the highest dose of 500 mg/kg. The lesion formation reduces in all the methods used followed by a significant increase of mucus production. The pylorus ligation technic revealed that the extract has an antisecretory activity.

Conclusions

The methanolic extract of D. benthamianus stem bark has both cytoprotective and antisecretory effects. This extract exerts its antisecretory effect trough cholinergic and histaminergic pathways.

Keywords: antisecretion; cytoprotection; Distemonanthus benthamianus; gastric ulcers

Corresponding author: Pr. Gilbert Ateufack, Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon, Phone: +237 675277614, E-mail: ateufack2000@yahoo.fr

Acknowledgements

We gratefully acknowledge University of Dschang, through the Laboratory of Animal Physiology and Phytopharmacology that provided general support for this work.

Research funding: The study was not supported by any program.
Availability of data and materials: All data supporting our findings are adequately contained within the manuscript.
Consent for publication: A statement regarding consent for publication is not applicable for this study.
Data availability: All data supporting our findings are adequately contained within the manuscript.
Author contributions: MMVM, AG and MM collected plant material, performed pharmacological assays, statistical analysis and prepared the manuscript. MMVM, AG, MM, AAD, TTH, EJ, MC and YNW conceived, revised the manuscript and supervised the study. MM and YNW helped in raw data analysis and provided punctual assistance. ACF, DNSF, TEG and TTH contributed in plant collection and laboratory work. All authors critically read and approved the final manuscript for submission.
Competing interests: The authors declare that they have no competing interests.

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Received: 2019-08-15

Accepted: 2020-04-02

Published Online: 2020-07-24

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Artikel in diesem Heft

https://doi.org/10.1515/jcim-2019-0216

Schlagwörter für diesen Artikel

antisecretion; cytoprotection; Distemonanthus benthamianus; gastric ulcers