Structural characterization, antioxidant, and antibiofilm activities of Coffea canephora green seeds
-
Mohammed Aissaoui
,
Hassan Latrache
Abstract
Objectives
In order to explore Coffea canephora green seeds as natural extract for application in the functional-food industry, we focused this study to the evaluation of the antioxidant and the antiadhesion effect of C. canephora green seeds extracts.
Methods
The analysis of C. canephora green seeds extracts was carried out by RP-HPLC-PDA. These extracts were screened for antioxidant activities by ABTS and phenanthroline assays. The antibacterial activity was determined by microdilution method against three reference bacteria. The inhibition of bacterial adhesion at 1/8 MIC was carried out against three reference bacteria.
Results
The RP-HPLC-PDA revealed the presence of gallic acid, vanillin, quercetin, chlorogenic acid, and P-coumaric acid. The n-buatnol extract have the highest activity ABTS assays (3.96 ± 0.08 μg/mL). For this extract, the A0.5 was 1.90 ± 0.05 μg/mL for phenanthroline assay. The n-butanol extract and the methanolic extract have the higher antibacterial activity against Staphylococcus aureus ATCC 25923 (40 µg/mL).At MIC/8, the extracts of C. canephora showed 70% higher antidhesive activity against S. aureus ATCC 25923.
Conclusions
Our finding provides an effective and specific new approach to the search of antioxidant and antiadhesive compounds for different uses.
Acknowledgments
The authors are grateful to Pr Atik-Bekkara F., director of Natural Product laboratory, for his assistance. We thank DGRSDT and the University of Tlemcen-Algeria for funding this work.
Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all individuals included in this study.
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
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Artikel in diesem Heft
- Frontmatter
- Reviews
- Endocannabinoids release after Osteopathic Manipulative Treatment. A brief review
- A pervasive scientific overview on mangiferin in the prevention and treatment of various diseases with preclinical and clinical updates
- Clinical trials of homeopathy in urological disorders: a systematic review
- Strategies in traditional Persian medicine to maintain a healthy life in the elderly
- Research articles Basic
- Cytoprotective and antisecretory properties of methanolic extract of Distemonanthus benthamianus (Caesalpiniaceae) stem bark on acute gastric ulcer in rats
- Screening chemical inhibitors for alpha-amylase from leaves extracts of Murraya koenigii (Linn.) and Aegle marmelos L.
- Ayurvedic formulations containing benzoic and ascorbic acids as additives: benzene formation during storage and impact of additives on quality parameters
- High cholesterol diet promotes dysfunction of arginase and cholinergic enzymatic system in rats: ameliorative role of caffeic and chlorogenic acids
- Cytokine and inflammatory mediators are associated with cytotoxic, anti-inflammatory and apoptotic activity of honeybee venom
- Anti-anaemic effect of ethanol leaf extract of Cnidosculus aconitifolius on cyclophosphomide-induced anaemia in rats
- Alterations in histological, biochemical and hematological parameters in Plasmodium berghei NK-65 infected balb/c mice treated with Bridelia ferruginea stem bark extract
- Structural characterization, antioxidant, and antibiofilm activities of Coffea canephora green seeds
- Research articles Clinical
- Safety, efficacy and tolerability of a combination micronutrient and polyherbal preparation (GoutFighterTM) for gout: a single-arm open-label pilot study
- Efficacy of Habbul aas (fruits of Myrtus communis) in menorrhagia: a single blinded randomized standard control study
- Ginger (Zingiber officinale) and turmeric (Curcuma longa L.) supplementation effects on quality of life, body composition, bone mineral density and osteoporosis related biomarkers and micro-RNAs in women with postmenopausal osteoporosis: a study protocol for a randomized controlled clinical trial
- Lithotriptic effect of Safūf Hajar-al Yahūd in patients of Hasat-ul Kilya (Nephrolithiasis) – an open prospective clinical validation trial
- Efficacy of Qurs-e-Gulnar in Ziabetus (type 2 Diabetes Mellitus): a single blind randomized controlled trial
- Immediate effect of Kapalbhathi pranayama on short term heart rate variability (HRV) in healthy volunteers
- Designing and validation of a yoga-based module for obesity with metabolic comorbidities
- A mixed-method randomized feasibility trial evaluating progressive muscle relaxation or autogenic training on depressive symptoms and quality of life in people living with human immunodeficiency virus (HIV) who have depressive symptoms
- Analysis of the bio-psychological characteristics of Sasang typology in Korean preschool children using the ponderal index and the temperament and character inventory
- Complementary and alternative medicine is positively associated with religiousness/spirituality
- Comparison the effects of inhalation and massage aromatherapy with lavender and sweet orange on fatigue in hemodialysis patients: a randomized clinical trial
- Improvement in resilience and stress-related blood markers following ten months yoga practice in Antarctica
- Neutral douche: a hydrotherapeutic tool to manage pain and systemic symptoms in primary dysmenorrhea - a randomised controlled study
- Short Communication
- The effects of omega-3 on the sleep quality of patients with uremic pruritus undergoing hemodialysis: a randomized crossover study
- Case Reports
- Āyuṛveda management of keratosis pilaris – a case report
- Improvement in impaired ocular accommodation by parietal acupoint therapy to relieve neck stiffness: a case report
