Screening chemical inhibitors for alpha-amylase from leaves extracts of Murraya koenigii (Linn.) and Aegle marmelos L.

Alagar Yadav Sangilimuthu; Thirunavukkarasu Sivaraman; Rajkuberan Chandrasekaran; Karpagavalli Meenatchi Sundaram; Gayathiri Ekambaram

doi:10.1515/jcim-2019-0345

Article

Screening chemical inhibitors for alpha-amylase from leaves extracts of Murraya koenigii (Linn.) and Aegle marmelos L.

Alagar Yadav Sangilimuthu , Thirunavukkarasu Sivaraman , Rajkuberan Chandrasekaran , Karpagavalli Meenatchi Sundaram and Gayathiri Ekambaram

Published/Copyright: August 3, 2020

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From the journal Journal of Complementary and Integrative Medicine Volume 18 Issue 1

Abstract

Objectives

Aqueous leaves extracts of Murraya koenigii (M. koenigii) and Aegle marmelos (A. marmelos) were prepared and effect of the extracts on inhibiting alpha-amylase playing essential roles on converting starch into glucose have been examined using in vitro assays.

Methods

Alpha amylase inhibitory assay was used to asses the in vitro antidiabetic activity of the extracts. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify the volatile molecules of the extracts. Identified molecule were converted as ligand and docked against human pancreatic α-amylase (0.95 Å; PDB ID: 5U3A) using Autodock tool.

Results

The data analyzes suggested that the alpha-amylase inhibition potential of the extract obtained from M. koenigii was stronger than that of the A. marmelos at low concentrations (<1 mg/mL), whereas both the extracts depicted similar inhibition effects on the enzyme at high concentration (>1 mg/mL). The phytochemicals present in both the plant extracts were identified by using their respective GC-MS data and the data analyzes revealed that the extracts of M. koenigii and A. Marmelos seemed to consist of about 20 and 24 diverse chemical molecules, respectively. Through the molecular docking studies, azulene of M. koenigii and hydroxycyclodecadiene of A. marmelos showed higher binding affinity on alpha-amylase.

Conclusions

Concentration-dependent alpha-amylase inhibition effects of the extracts were observed and M. koenigii contains more alpha-amylase inhibitory effects due to the presence of azulene. This is primary lead to find out the better anti diabetic natural based drug to the society after clinical trial.

Keywords: Aegle marmelos; alpha-amylase; anti-diabetic; HTVS; Murraya koenigii

Corresponding author: Dr. Alagar Yadav Sangilimuthu, Associate Professor and Head, Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, 641021, Tamil Nadu, India, E-mail: smuthu.al@gmail.com; and

Corresponding author: Thirunavukkarasu Sivaraman, Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, 641021, Tamil Nadu, India, E-mail: eazhilarasen@yahoo.com

Acknowledgments

The authors acknowledge the dignitaries of Karpagam Academy of Higher Education for providing access to utilize the ‘Central Instrumentation Facility’ (CIF-KAHE) to carry out biophysical and biochemical experiments of the research work.

Research funding: The authors gratefully acknowledge Department of Science and Technology, New Delhi funds (SR/FST/LS-1/2018/187) for this research.
Author contributions: SAY carried out the GC analysis presented in the paper and wrotethe manuscript; TS performed the computational parts of the works and also contributed to the text of the manuscript. GE prepared the plants extracts and executed the in vitro assays presented in the paper.
Competing interests: There is no conflict of interest.

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Received: 2020-01-12

Accepted: 2020-04-07

Published Online: 2020-08-03

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Articles in the same Issue

https://doi.org/10.1515/jcim-2019-0345

Keywords for this article

Aegle marmelos; alpha-amylase; anti-diabetic; HTVS; Murraya koenigii