Home Medicine Cytokine and inflammatory mediators are associated with cytotoxic, anti-inflammatory and apoptotic activity of honeybee venom
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Cytokine and inflammatory mediators are associated with cytotoxic, anti-inflammatory and apoptotic activity of honeybee venom

  • Mohamed A. Salama , Mohamed A. Younis and Roba M. Talaat ORCID logo EMAIL logo
Published/Copyright: May 26, 2020
Journal of Complementary and Integrative Medicine
From the journal Journal of Complementary and Integrative Medicine Volume 18 Issue 1

Abstract

Objective

The present study aimed to evaluate cytotoxic, apoptotic, and anti-inflammatory properties of bee venom (BV) as well as changes in cytokine secretion levels and nitric oxide (NO) production using three different cancer cell lines [liver (Hep-G2), breast (MCF-7), and cervical (HPV-18 infected HeLa cells)] and two normal cells (splenocytes and macrophages (MQ).

Methods

Cytotoxic activity of BV against tumor cell lines and normal splenocytes/MQ was tested by MTT assay. By ELISA (ELISA); Tumor necrosis factor (TNF-α), Interleukine (IL-10) and interferon (IFN-γ) were measured. Caspase three expressions was evaluated using reverse transcription-polymerase chain reaction (RT-PCR). Nitric oxide (NO) was estimated using a colorimetric assay.

Results

BV has a significant cytotoxic effect on all cell lines in a dose- and time-dependent manner; none of them was toxic for normal cells. Treating Hep-G2 cells with BV showed a reduction in IL-10, elevation in TNF-α with no change in IFN-γ level. MCF-7 cells have low IL-10 and TNF-α and high IFN-γ production level. Elevation of IL-10 and IFN-γ coincides with a reduction in TNF-α level was demonstrated in HeLa cells. The expression of Caspase three was dramatically increased with elevation in BV concentration in all tested cancer cell lines. A gradual decrease in NO production by MQ with increasing BV dose was observed.

Conclusion

Taken together, our results stressed on the importance of BV as a potent anti-tumor agent against various types of cancers (Liver, Breast, and Cervix). Further steps towards the use of BV for pharmacological purposes must be done.

Keywords: BV; cytotoxicity; Hep-G2; HeLa; inflammation; NO; MCF-7
Corresponding author: Dr. Roba M. Talaat, Prof., Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat, Egypt. Phone: +2 048 260 1264/65, Fax: +2 048 260 1266/68, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: None declared.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

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Received: 2019-07-24
Accepted: 2020-01-12
Published Online: 2020-05-26

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jcim-2019-0182
Keywords for this article
BV; cytotoxicity; Hep-G2; HeLa; inflammation; NO; MCF-7

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Endocannabinoids release after Osteopathic Manipulative Treatment. A brief review
  4. A pervasive scientific overview on mangiferin in the prevention and treatment of various diseases with preclinical and clinical updates
  5. Clinical trials of homeopathy in urological disorders: a systematic review
  6. Strategies in traditional Persian medicine to maintain a healthy life in the elderly
  7. Research articles Basic
  8. Cytoprotective and antisecretory properties of methanolic extract of Distemonanthus benthamianus (Caesalpiniaceae) stem bark on acute gastric ulcer in rats
  9. Screening chemical inhibitors for alpha-amylase from leaves extracts of Murraya koenigii (Linn.) and Aegle marmelos L.
  10. Ayurvedic formulations containing benzoic and ascorbic acids as additives: benzene formation during storage and impact of additives on quality parameters
  11. High cholesterol diet promotes dysfunction of arginase and cholinergic enzymatic system in rats: ameliorative role of caffeic and chlorogenic acids
  12. Cytokine and inflammatory mediators are associated with cytotoxic, anti-inflammatory and apoptotic activity of honeybee venom
  13. Anti-anaemic effect of ethanol leaf extract of Cnidosculus aconitifolius on cyclophosphomide-induced anaemia in rats
  14. Alterations in histological, biochemical and hematological parameters in Plasmodium berghei NK-65 infected balb/c mice treated with Bridelia ferruginea stem bark extract
  15. Structural characterization, antioxidant, and antibiofilm activities of Coffea canephora green seeds
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  17. Safety, efficacy and tolerability of a combination micronutrient and polyherbal preparation (GoutFighterTM) for gout: a single-arm open-label pilot study
  18. Efficacy of Habbul aas (fruits of Myrtus communis) in menorrhagia: a single blinded randomized standard control study
  19. Ginger (Zingiber officinale) and turmeric (Curcuma longa L.) supplementation effects on quality of life, body composition, bone mineral density and osteoporosis related biomarkers and micro-RNAs in women with postmenopausal osteoporosis: a study protocol for a randomized controlled clinical trial
  20. Lithotriptic effect of Safūf Hajar-al Yahūd in patients of Hasat-ul Kilya (Nephrolithiasis) – an open prospective clinical validation trial
  21. Efficacy of Qurs-e-Gulnar in Ziabetus (type 2 Diabetes Mellitus): a single blind randomized controlled trial
  22. Immediate effect of Kapalbhathi pranayama on short term heart rate variability (HRV) in healthy volunteers
  23. Designing and validation of a yoga-based module for obesity with metabolic comorbidities
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