Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract
-
Olufunmilayo O. Adeyemi
,
Ismail O. Ishola
Abstract
Background
Tetracera alnifolia Willd. (Dilleniaceae) is used in traditional African Medicine for the treatment of headache, abdominal pain, and rheumatism. Hence, this study sought to investigate the antinociceptive and anti-inflammatory effects of the hydroethanolic leaf extract of T. alnifolia (HeTA) in rodents.
Methods
Antinociceptive activity was evaluated using the acetic acid-induced writhing, formalin-/capsaicin-induced paw licking and hot plate tests in mice. The contribution of opioidergic, l-arginine-nitric oxide, and ATP-sensitive potassium channel pathways in HeTA-induced antinociception was also evaluated. The anti-inflammatory effect was assessed using the carrageenan-induced paw edema, xylene ear edema, cotton pellet granuloma, and complete Freund’s adjuvant (CFA)-induced arthritis in rats.
Results
HeTA (100, 200, and 400 mg/kg, p.o.) produced significant (p<0.05) decrease in mean number of acetic acid-induced writhing, time spent licking paw in formalin, and capsaicin tests as well as time course increase in nociceptive reaction latency in hot plate test. HeTA-induced antinociception was prevented by pretreatment of mice with naloxone (non-selective opioid receptor antagonist), l-arginine (nitric oxide precursor), or glibenclamide (ATP-sensitive potassium channel blocker). HeTA (100 mg/kg, p.o.) produced a significant anti-inflammatory effect against carrageenan-induced rat paw edema (1–5 h), xylene-induced ear edema, cotton pellet-induced granuloma formation, and CFA-induced arthritis in rats. The effects of HeTA in various models were similar to the effect of the standard reference drugs.
Conclusions
Findings from this study showed that HeTA possesses antinociceptive effect possibly mediated through peripheral opioid receptors with activation of l-arginine-nitric oxide and ATP-sensitive potassium channel pathway as well as anti-inflammatory activity.
Acknowledgments
We acknowledged the technical assistance rendered by Mr. C. Micah of the Department of Pharmacology, Therapeutics and Toxicology, College of Medicine of the University of Lagos, Lagos, Nigeria
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Dewanjee S, Maiti A, Sahu R, Dua TK, Mandal SC. Study of anti-inflammatory and antinociceptive activity of hydroalcoholic extract of Schima wallichii bark. Pharm Biol 2009;47:402–7.10.1080/13880200902758824Suche in Google Scholar
2. Ray SD, Ray S, Zia-Ul-Haq M, De Feo V, Dewanjee S. Pharmacological basis of the use of the root bark of Zizyphus nummularia Aubrev. (Rhamnaceae) as anti-inflammatory agent. BMC Compl Altern Med 2015;15:416.10.1186/s12906-015-0942-7Suche in Google Scholar
3. Aronson JK. Meyler’s side effects of analgesics and anti-inflammatory drugs. Amsterdam: Elsevier, 2009.Suche in Google Scholar
4. Ishola IO, Akindele AJ, Adeyemi OO. Analgesic and anti-inflammatory activities of Cnestis ferruginea Vahl ex DC (Connaraceae) methanolic root extract. J Ethnopharmacol 2011;135:55–62.10.1016/j.jep.2011.02.024Suche in Google Scholar
5. Ishola IO, Ikumawoyi VO, Afolayan GO, Olorife OJ. Antinociceptive and anti-inflammatory properties of hydroethanolic seed extract of Monodora myristica (Annonaceae) in rodents. West Afr J Pharm 2016;27:22–32.Suche in Google Scholar
6. Shah SM, Shah SM. Phytochemicals, antioxidant, antinociceptive and anti-inflammatory potential of the aqueous extract of Teucrium stocksianum bioss. BMC Compl Altern Med 2015;15:351.10.1186/s12906-015-0872-4Suche in Google Scholar
7. Burkill HM. The useful plants of West Tropical Africa, vol. 1. Kew: Royal Botanic Gardens, 1985;15.Suche in Google Scholar
8. Bouquet A, Debray M. Plantes medicinales de la Cote d. Ivoire, Trav. Doc. ORSTOM. 1974;32.Suche in Google Scholar
9. NIH. Guide for the use of laboratory animals DHHS, PHS. NIH Publication No. 85-23 (1985 Revised).Suche in Google Scholar
10. Harborne JB. Phytochemical methods a guide to modern techniques of plant analysis. London, New York: Chapman and Hall Ltd, 1973;49–188.Suche in Google Scholar
11. Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 2003;81:321–6.10.1016/S0308-8146(02)00423-5Suche in Google Scholar
12. Atsumi T, Iwakura I, Kashiwagi Y, Fujisawa S, Ueha T. Free radical scavenging activity in the nonenzymatic fraction of human saliva: a simple DPPH assay showing the effect of physical exercise. Antioxid Redox Signal 1999;1:537–46.10.1089/ars.1999.1.4-537Suche in Google Scholar
13. Leone AM, Francis PL, Rhodes P, Moncada S. A rapid and simple method for the measurement of nitrite and nitrate in plasma by high performance capillary electrophoresis. Biochem Biophysics Res Comm 1994;200:951–7.10.1006/bbrc.1994.1542Suche in Google Scholar
14. Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Compl Alternat Med 2010;10:1–8.10.1186/1472-6882-10-21Suche in Google Scholar
15. OECD. Guidance document on acute oral toxicity. Environmental Health and Safety Monograph Series on Testing and Assessment No. 24, 2000.Suche in Google Scholar
16. Ishola IO, Akinyede AA, Sholarin AM. Antidepressant and anxiolytic properties of the methanolic extract of Momordica charantia Linn (Cucurbitaceae) and its mechanism of action. Drug Res (Stuttg) 2014;64:368–76.10.1055/s-0033-1358712Suche in Google Scholar
17. Shibata M, Ohkubo T, Takahashi H, Inoki R. Modified formalin test: characteristic biphasic pain response. Pain 1989;38:347–52.10.1016/0304-3959(89)90222-4Suche in Google Scholar
18. Sakurada T, Katsumata K, Yogo H, Tan-No K, Sakurada S, Kisara K. Antinociception induced by CP 96,345, a non-peptide NK-1 receptor antagonist, in the mouse formalin and capsaicin tests. Neurosci Lett 1993;151:142–5.10.1016/0304-3940(93)90006-7Suche in Google Scholar
19. Eddy NB, Leimbach D. Systemic analgesics. II. Dithienylbutenyl and dithienylbutylamines. J Pharmacol Exp Ther 1953;107: 385–93.Suche in Google Scholar
20. Rodrigues AR, Duarte ID. The peripheral antinociceptive effect induced by morphine is associated with ATP-sensitive K+ channels. Br J Pharmacol 2000;129:110–4.10.1038/sj.bjp.0703038Suche in Google Scholar
21. Ishola IO, Awodele O, Olusayero AM, Ochieng CO. Mechanisms of analgesic and anti-inflammatory properties of Annona muricata Linn. (Annonaceae) fruit extract in rodents. J Med Food 2014;17:1375–82.10.1089/jmf.2013.0088Suche in Google Scholar
22. Galdino GS, Xavier CH, Almeida R, Silva G, Fontes MA, Menezes G, et al. The nitric oxide/CGMP/KATP pathway mediates systemic and central antinociception induced by resistance exercise in rats. Int J Neurosci 2015;125:765–73.10.3109/00207454.2014.970256Suche in Google Scholar
23. Venkataramanan PE, Parvathavarthini S, Viswanathan S. Role of ATP sensitive potassium channel on 7-hydroxy flavone induced antinociception and possible association with changes in glycaemic status. Indian J Exp Biol 2000;38:1172–4.Suche in Google Scholar
24. Winter CA, Risley EA, Nuss GW. Carrageenan-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med 1962;111:544–7.10.3181/00379727-111-27849Suche in Google Scholar
25. Ashford A, Penn GB. Effect of adrenalectomy on cotton pellet granuloma formation in the rat. J Pharm Pharmacol 1965;17:10–4.10.1111/j.2042-7158.1965.tb07559.xSuche in Google Scholar
26. Ishola IO, Akinyede A, Lawal SM, Popoola TD. Antinociceptive and anti-inflammatory effects of Olax subscorpioidea Oliv.(Olacaceae) leaf extract in rodents: possible mechanisms of antinociceptive action. West Afr J Pharm 2015;26:99–112.Suche in Google Scholar
27. Deraedt R, Jouquey S, Delevallée F, Flahaut M. Release of prostaglandins E and F in an algogenic reaction and its inhibition. Eur J Pharmacol 1980;61:17–24.10.1016/0014-2999(80)90377-5Suche in Google Scholar
28. Ikeda Y, Ueno A, Naraba H, Oh-ishi S. Involvement of vanilloid receptor VR1 and prostanoids in the acid-induced writhing responses of mice. Life Sci 2001;69:2911–9.10.1016/S0024-3205(01)01374-1Suche in Google Scholar
29. Martínez V, Thakur S, Mogil JS, Taché Y, Mayer EA. Differential effects of chemical and mechanical colonic irritation on behavioral pain response to intraperitoneal acetic acid in mice. Pain 1999;81:179–86.10.1016/S0304-3959(99)00008-1Suche in Google Scholar
30. Tjølsen A, Berge OG, Hunskaar S, Rosland JH, Hole K. The formalin test: an evaluation of the method. Pain 1992;51:5–17.10.1016/0304-3959(92)90003-TSuche in Google Scholar
31. Abbott FV, Franklin KB, Westbrook RF. The formalin test: scoring properties of the first and second phases of the pain response in rats. Pain 1995;60:91–102.10.1016/0304-3959(94)00095-VSuche in Google Scholar
32. Wood JN, Winter J, James IF, Rang HP, Yeats J, Bevan S. Capsaicin-induced ion fluxes in dorsal root ganglion cells in culture. J Neurosci 1988;8:3208–20.10.1523/JNEUROSCI.08-09-03208.1988Suche in Google Scholar
33. Caterina MJ, Leffler A, Malmberg AB, Martin WJ, Trafton J, Petersen-Zeitz KR, et al. Impaired nociception and pain sensation in mice lacking the capsaicin receptor. Science 2000;288:306–13.10.1126/science.288.5464.306Suche in Google Scholar
34. Fattori V, Hohmann MS, Rossaneis AC, Pinho-Ribeiro FA, Verri WA. Capsaicin: current understanding of its mechanisms and therapy of pain and other pre-clinical and clinical uses. Molecules 2016;21:844–76.10.3390/molecules21070844Suche in Google Scholar
35. Yang Y, Yang H, Wang Z, Varadaraj K, Kumari SS, Mergler S, et al. Cannabinoid receptor 1 suppresses transient receptor potential vanilloid 1-induced inflammatory responses to corneal injury. Cell Signal 2013;25:501–11.10.1016/j.cellsig.2012.10.015Suche in Google Scholar
36. Le Bars D, Gozariu M, Cadden SW. Animal models of nociception. Pharmacol Rev 2001;53:597–652.Suche in Google Scholar
37. Duarte ID, Lorenzetti BB, Ferreira SH. Peripheral analgesia and activation of the nitric oxide-cyclic GMP pathway. Eur J Pharmacol 1990;186:289–93.10.1016/0014-2999(90)90446-DSuche in Google Scholar
38. Ferreira SH, Duarte ID, Lorenzetti BB. The molecular mechanism of action of peripheral morphine analgesia: stimulation of the cGMP system via nitric oxide release. Eur J Pharmacol 1991;201:121–2.10.1016/0014-2999(91)90333-LSuche in Google Scholar
39. Vinegar R, Schreiber W, Hugo R. Biphasic development of carrageenan edema in rats. J Pharmacol Exp Ther 1969;166: 96–103.Suche in Google Scholar
40. Di Rosa M, Giroud JP, Willoughby DA. Studies of mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. J Pathol 1971;104:15–29.10.1002/path.1711040103Suche in Google Scholar PubMed
41. Seibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W, et al. Pharmacological and biochemical demonstration of the role of cyclooxygenase-2 in inflammation and pain. Proc Natl Acad Sci USA 1994;91:12013–7.10.1073/pnas.91.25.12013Suche in Google Scholar PubMed PubMed Central
42. Salvemini D, Wang ZQ, Wyatt PS, Bourdon DM, Marino MH, Manning PT, et al. Nitric oxide: a key mediator in the early and late phase of carrageenan-induced rat paw inflammation. Br J Pharmacol 1996;118:829–38.10.1111/j.1476-5381.1996.tb15475.xSuche in Google Scholar PubMed PubMed Central
43. Banki E, Hajna Z, Kemeny A, Botz B, Nagy P, Bolcskei K, et al. The selective PAC1 receptor agonist maxadilan inhibits neurogenic vasodilation and edema formation in the mouse skin. Neuropharmacology 2014;85:538–47.10.1016/j.neuropharm.2014.06.019Suche in Google Scholar
44. Torres-Rêgo M, Furtado AA, Bitencourt MA, Lima MC, Andrade RC, Azevedo EP, et al. Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae). BMC Complement Altern Med 2016;16:275.10.1186/s12906-016-1259-xSuche in Google Scholar
45. Swingle KF, Shideman FE. Phases of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents. J Pharmacol Exp Ther 1972;183:226–34.Suche in Google Scholar
46. Calvino B, Crepon-Bernard MA, Le Bars D. Parallel clinical and behavioral studies of adjuvant-induced arthritis in the rat: possible relationship with “chronic pain”. Behav Brain Res 1987;24:11–29.10.1016/0166-4328(87)90032-5Suche in Google Scholar
47. Liu YL, Lin HM, Zou R, Wu JC, Han R, Raymond LN, et al. Suppression of complete Freund’s adjuvant-induced adjuvant arthritis by cobratoxin. Acta Pharmacol Sin 2009;30:219–27.10.1038/aps.2008.20Suche in Google Scholar PubMed PubMed Central
48. Kim YW, Zhao RJ, Park SJ, Lee JR, Cho IJ, Yang CH, et al. Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-kappaB-dependent iNOS and proinflammatory cytokines production. Br J Pharmacol 2008;154:165–73.10.1038/bjp.2008.79Suche in Google Scholar PubMed PubMed Central
49. Fang J. Classification of fruits based on anthocyanin types and relevance to their health effects. Nutrition 2015;31:1301–6.10.1016/j.nut.2015.04.015Suche in Google Scholar PubMed
50. Elisha IL, Dzoyem JP, McGaw LJ, Botha FS, Eloff JN. The anti-arthritic, anti-inflammatory, antioxidant activity and relationships with total phenolics and total flavonoids of nine South African plants used traditionally to treat arthritis. BMC Complement Alt Med 2016;16:307.10.1186/s12906-016-1301-zSuche in Google Scholar PubMed PubMed Central
©2019 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Oxidative Stress
- Primary concept of nickel toxicity – an overview
- Behavior and Neuroprotection
- Review of thiamine deficiency disorders: Wernicke encephalopathy and Korsakoff psychosis
- Infection
- Opportunistic infection at the start of antiretroviral therapy and baseline CD4+ count less than 50 cells/mm3 are associated with poor immunological recovery
- Inflammation
- Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract
- Oxidative Stress
- Influence of eugenol on oxidative stress biomarkers in the liver of carrageenan-induced arthritis rats
- Selective serotonin reuptake inhibitor fluvoxamine ameliorates stress- and NSAID-induced peptic ulcer possibly by involving Hsp70
- The ethanol leaf extract of Andrographis paniculata blunts acute renal failure in cisplatin-induced injury in rats through inhibition of Kim-1 and upregulation of Nrf2 pathway
- Effect of l-carnitine on aspartame-induced oxidative stress, histopathological changes, and genotoxicity in liver of male rats
- Reproduction
- Sympathovagal imbalance and neurophysiologic cognitive assessment using evoked potentials in polycystic ovary syndrome in young adolescents – a cross-sectional study
- Phytotherapy
- Antidiabetic and antihyperlipidemic potential of ethanol extract of Salacia lehmbachii stem bark in alloxan-induced diabetic rats
- Hypoglycemic and antioxidant effects of oral administration of garlic extract in the livers of type 1 diabetic rats
- Anabasis aretioides Coss. & Moq. phenolic compounds exhibit in vitro hypoglycemic, antioxidant and antipathogenic properties
- Cardiovascular Function
- Cardioautonomic responses to acute ingestion of ice water and its correlation to body mass index
- Metabolism
- Repeated restraint stress upregulates rat sulfotransferase 1A1
- Case Report
- Tramadol-induced parkinsonism: a case report of a 75-year-old woman
Artikel in diesem Heft
- Frontmatter
- Oxidative Stress
- Primary concept of nickel toxicity – an overview
- Behavior and Neuroprotection
- Review of thiamine deficiency disorders: Wernicke encephalopathy and Korsakoff psychosis
- Infection
- Opportunistic infection at the start of antiretroviral therapy and baseline CD4+ count less than 50 cells/mm3 are associated with poor immunological recovery
- Inflammation
- Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract
- Oxidative Stress
- Influence of eugenol on oxidative stress biomarkers in the liver of carrageenan-induced arthritis rats
- Selective serotonin reuptake inhibitor fluvoxamine ameliorates stress- and NSAID-induced peptic ulcer possibly by involving Hsp70
- The ethanol leaf extract of Andrographis paniculata blunts acute renal failure in cisplatin-induced injury in rats through inhibition of Kim-1 and upregulation of Nrf2 pathway
- Effect of l-carnitine on aspartame-induced oxidative stress, histopathological changes, and genotoxicity in liver of male rats
- Reproduction
- Sympathovagal imbalance and neurophysiologic cognitive assessment using evoked potentials in polycystic ovary syndrome in young adolescents – a cross-sectional study
- Phytotherapy
- Antidiabetic and antihyperlipidemic potential of ethanol extract of Salacia lehmbachii stem bark in alloxan-induced diabetic rats
- Hypoglycemic and antioxidant effects of oral administration of garlic extract in the livers of type 1 diabetic rats
- Anabasis aretioides Coss. & Moq. phenolic compounds exhibit in vitro hypoglycemic, antioxidant and antipathogenic properties
- Cardiovascular Function
- Cardioautonomic responses to acute ingestion of ice water and its correlation to body mass index
- Metabolism
- Repeated restraint stress upregulates rat sulfotransferase 1A1
- Case Report
- Tramadol-induced parkinsonism: a case report of a 75-year-old woman
