Influence of eugenol on oxidative stress biomarkers in the liver of carrageenan-induced arthritis rats
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Abstract
Background
Eugenol is the foremost constituent of clove oil and widely distributed in many plants. It possesses many pharmaceutical applications, including antioxidant, anti-inflammatory, and anti-tumorigenic properties, among others. This study evaluates the influence of eugenol on oxidative stress biomarkers in the liver of carrageenan-induced arthritis (CIA) rats.
Methods
Sixty albino rats were randomly divided into 10 (n=6) groups. Group I is the control group that received saline solution orally. Groups II and VII rats received 2.5 mg/kg of eugenol orally (EUG-2.5). Rats in groups III/VIII and IV/IX received 5 and 10 mg/kg of eugenol orally (EUG-5 and EUG-10), respectively. Groups V and X received 0.2 mg/kg of dexamethasone (DEX-0.2) orally. Groups VI to X were injected with 1% carrageenan intra-articularly. Behavioral studies were conducted after 21 days of treatment. Thereafter, the animals were sacrificed, and the livers were isolated and used for biochemical analysis.
Results
Reduced body weight in arthritic rats was recorded compared to normal controls. Reduced tibiofemoral joint edema and increased spontaneous movement were observed in CIA rats with decreased superoxide dismutase, catalase, reduced glutathione (GSH), glutathione peroxidase, and GSH S-transferase activities compared with the normal control group. Increased endogenous enzyme activities and decreased elevated lipid peroxidation were also observed after eugenol treatment.
Conclusion
Eugenol ameliorates carrageenan-induced oxidative stress in the liver of arthritic rats.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Bendele AM. Animal models of rheumatoid arthritis. J Musculoskelet Neuronal Interact 2001;1:377–85.Suche in Google Scholar
2. Comar JF, Babeto de Sá-Nakanishi A, Oliveira AL, Wendt MM, Amado CA, Iwamoto EL, et al. Oxidative state of the liver of rats with adjuvant-induced arthritis. Free Radical Biol Med 2012;58:144–53.10.1016/j.freeradbiomed.2012.12.003Suche in Google Scholar
3. Haruna Y, Morita Y, Yada T, Satoh M, Fox DA, Kashihara N. Fluvastatin reverses endothelial dysfunction and increased vascular oxidative stress in rat adjuvant-induced arthritis. Arthritis Rheum 2007;56:1827–35.10.1002/art.22632Suche in Google Scholar
4. Mathew LE, Rajagopol V, Pushpan CK, Helen A. Fluvastatin alleviates inflammation and oxidative stress in adjuvant induced arthritic rats by the down regulation of TNF-α and IL-6. Pharmacologia 2013;4:498–506.10.5567/pharmacologia.2013.498.506Suche in Google Scholar
5. Flora SJ. Role of free radicals and antioxidants in health and disease. Cell Mol Biol 2007;53:1–2.Suche in Google Scholar
6. Mirshafiey A, Mohsenzadegan M. The role of reactive oxygen species in immunopathogenesis of rheumatoid arthritis. Iran J Allergy Asthma Immunol 2008;7:195–202.Suche in Google Scholar
7. Petrov L, Tzvetanova E, Pavlova A, Alexandrova A, Zamfirova R, Kirkova M, et al. In-vivo effects of nociceptin and its structural analogue nociceptin on the antioxidant status of rat blood and liver after carrageenan-induced paw inflammation. Central Eur J Med 2010;5:123–31.10.2478/s11536-009-0117-3Suche in Google Scholar
8. Atkinson AJ, Colburn WA, De Gruttola VG, De Mets DL, Downing GJ, Hoth DF, et al. Biomarkers and surrogate endpoints: preferred definitions and conceptual framework. Clin Pharmacol Ther 2001;69:89–95.10.1067/mcp.2001.113989Suche in Google Scholar
9. Mayne ST. Antioxidant nutrients and chronic disease: use of biomarkers of exposure and oxidative stress status in epidemiologic research. J Nutr 2003;133:933–40.10.1093/jn/133.3.933SSuche in Google Scholar
10. Cooke MS, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J 2003;17:1195–214.10.1096/fj.02-0752revSuche in Google Scholar
11. Gackowski D, Speina E, Zielinska M, Kowalewski J, Rozalski R, Siomek A, et al. Products of oxidative DNA damage and repair as possible biomarkers of susceptibility to lung cancer. Cancer Res 2003;63:4899–902.Suche in Google Scholar
12. Ilhan N, Ilhan N. Vascular endothelial growth factor and oxidative damage in cancer. Clin Biochem 2003;36:225–8.10.1016/S0009-9120(02)00450-2Suche in Google Scholar
13. Fujisawa S, Murakami Y. Eugenol and its role in chronic diseases. Adv Exp Med Biol 2016;929:45–66.10.1007/978-3-319-41342-6_3Suche in Google Scholar PubMed
14. Haqqi TM, Anthony DD, Gupta S, Ahmad N, Lee MS, Kumar GK, et al. Prevention of collagen-induced arthritis in mice by a polyphenolic fraction from green tea. Proc Natl Acad Sci 1999;96:4524–9.10.1073/pnas.96.8.4524Suche in Google Scholar PubMed PubMed Central
15. Prasad SN, Bharath MM, Muralidhara M. Neurorestorative effects of eugenol, a spice bioactive: evidence in cell model and its efficacy as an intervention molecule to abrogate brain oxidative dysfunctions in the streptozotocin diabetic rat. Neurochem Int 2016;95:24–36.10.1016/j.neuint.2015.10.012Suche in Google Scholar
16. Dubey K, Anand BG, Shekhawat DS, Kar K. Eugenol prevents amyloid formation of proteins and inhibits amyloid-induced hemolysis. Sci Rep 2017;7:40744.10.1038/srep40744Suche in Google Scholar
17. Goulart AC, dos Santos Correi FA, Machado de Sousa SC, de Cerqueira Luz JG. Study of the inflammatory process induced by injection of carrageenan or formalin in the rat temporomandibular joint. Braz Oral Res 2005;19:99–105.10.1590/S1806-83242005000200005Suche in Google Scholar
18. Srinivasan S, Sathish G, Jayanthi M, Muthukumaran J, Muruganathan U, Ramachandran V. Ameliorating effect of eugenol on hyperglycemia by attenuating the key enzymes of glucose metabolism in streptozotocin-induced diabetic rats. Mol Cell Biochem 2014;385:159–68.10.1007/s11010-013-1824-2Suche in Google Scholar
19. Sulaiman AA, Hussain SA, Jum’ma KM, Sigar AH. Evaluation of the anti-inflammatory effect of pioglitazone in experimental models of inflammation in rats. Iraqi J Pharm Sci 2009;18:1–6.10.31351/vol18issSuppl.pp1-6Suche in Google Scholar
20. Suzuki M, Hori S. Experimental investigation in rats to identify the cause of sudden death during bathing in Japan. Acute Med Surg 2014;1:101–4.10.1002/ams2.18Suche in Google Scholar
21. Kulkarni SK. Handbook of experimental pharmacology, 3rd ed. New Delhi, India: Vallabh Prakashan, 2005:148–50.Suche in Google Scholar
22. Misra HP, Fridovich I. The generation of superoxide radical during the antioxidant of hemoglobin. J Biol Chem 1972;247: 6960–2.10.1016/S0021-9258(19)44679-6Suche in Google Scholar
23. Asru KS. Colorimetric assay of catalase. Anal Biochem 1951;47:389–94.10.1016/0003-2697(72)90132-7Suche in Google Scholar
24. Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR. Bromobenzene induced liver necrosis: protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 1974;11:151–69.10.1159/000136485Suche in Google Scholar
25. Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat’s liver. Biochem Biophys Res Commun 1961;71:952–8.10.1016/0006-291X(76)90747-6Suche in Google Scholar
26. Habig WH, Pabst MJ, Jacoby WB. Glutathione-S-transferases: the first enzymatic step in mercaptoric acid formation. J Biol Chem 1974;249:7130–9.10.1016/S0021-9258(19)42083-8Suche in Google Scholar
27. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8.10.1016/0003-2697(79)90738-3Suche in Google Scholar
28. Bradford M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–54.10.1016/0003-2697(76)90527-3Suche in Google Scholar
29. Salvemini D, Riley DP. M40403, superoxide dismutase mimetic. Drugs Future 2000;25:1027–33.10.1358/dof.2000.025.10.594996Suche in Google Scholar
30. Biswas SK, Lopes de Faria JB. Hypertension induces oxidative stress but not macrophage infiltration in the kidney in the early stage of experimental diabetes mellitus. Am J Nephrol 2006;26:415–22.10.1159/000095707Suche in Google Scholar PubMed
31. Chandra K, Syed SA, Abid M, Najam AK. Protection against FCA induced oxidative stress induced DNA damage as a model of arthritis and in vitro anti-arthritic potential of Costus speciosus rhizome extract. Int J Pharmacog Phytochem Res 2015;7:383–9.Suche in Google Scholar
32. Subramanian N, Bray MA. Interleukin 1 releases histamine from human basophils and mast cells in vitro. J Immunol 1987;138:271–5.10.4049/jimmunol.138.1.271Suche in Google Scholar
33. Leonavičienė L, Bradūnaitė R, Vaitkienė D, Vasiliauskas A, Keturkienė A. Collagen-induced arthritis and pro-/antioxidant status in Wistar and Lewis rats. Biologija 2008;54:290–300.10.2478/v10054-008-0059-8Suche in Google Scholar
34. Hemshekhar M, Sebastin-Santhosh M, Sunitha K, Thushara RM, Kemparaju K, Rangappa KS. A dietary colorant crocin mitigates arthritis and associated secondary complications by modulating cartilage deteriorating enzymes, inflammatory mediators and antioxidant status. Biochimie 2010;94:2723–33.10.1016/j.biochi.2012.08.013Suche in Google Scholar PubMed
35. Walker AF, Bundy R, Hicks SM, Middleton RW. Bromelain reduces mild acute knee pain and improves well-being in a dose-dependent fashion in an open study of otherwise healthy adults. Phytomedicine 2002;9:681–6.10.1078/094471102321621269Suche in Google Scholar PubMed
36. Suresh P, Kavitha CN, Babu SM, Reddy VP, Latha AK. Effect of ethanol extract of Trigonella foenum graecum (Fenugreek) seeds on Freund’s adjuvant-induced arthritis in albino rats. Inflammation 2012;35:1314–21.10.1007/s10753-012-9444-7Suche in Google Scholar PubMed
37. Vetal S, Bodhankar SL, Mohan V, Thakurdesai PA. Anti-inflammatory and anti-arthritic activity of type-A procyanidine polyphenols from bark of Cinnamomum zeylanicum in rats. Food Sci Human Well 2013;2:59–67.10.1016/j.fshw.2013.03.003Suche in Google Scholar
38. Hayyan M, Hashim MA, Al-Nashef IM. Superoxide ion: generation and chemical implications. Chem Rev 2016;116:3029–85.10.1021/acs.chemrev.5b00407Suche in Google Scholar PubMed
39. Kshirsagar AD, Panchal PV, Harle UN, Nanda RK, Shaikh HM. Anti-inflammatory and antiarthritic activity of anthraquinone derivatives in rodents. Int J Inflamm 2014;2014:Article ID 690596, 12 pages.10.1155/2014/690596Suche in Google Scholar PubMed PubMed Central
40. Bejarano I, Espino J, González-Flores D, Casado JG, Redondo PC, Rosado JA, et al. Role of calcium signals on hydrogen peroxide induced apoptosis in human myeloid HL-60 cells. Int J Biomed Sci 2009;5:246–56.10.59566/IJBS.2009.5246Suche in Google Scholar
41. Mateen S, Moin S, Khan AQ, Zafar A, Fatima N. Increased reactive oxygen species formation and oxidative stress in rheumatoid arthritis. PLoS One 2016;11:e0152925.10.1371/journal.pone.0152925Suche in Google Scholar PubMed PubMed Central
42. Anderson AJ. Lysosomal enzyme activity in rats with adjuvant-induced arthritis. Ann Rheum Dis 1970;29:307–13.10.1136/ard.29.3.307Suche in Google Scholar PubMed PubMed Central
43. Filippin LI, Vercelino R, Marroni NP, Xavier RM. Redox signalling and the inflammatory response in rheumatoid arthritis. Clin Exp Immunol 2008;152:415–22.10.1111/j.1365-2249.2008.03634.xSuche in Google Scholar PubMed PubMed Central
44. Hassan MQ, Hadi RA, Al-Rawi ZS, Padron VA, Stohs SJ. The glutathione defense system in the pathogenesis of rheumatoid arthritis. J Appl Toxicol 2001;21:69–73.10.1002/jat.736Suche in Google Scholar PubMed
45. Kalpakcioglu B, Şenel K. The interrelation of glutathione reductase, catalase, glutathione peroxidase, superoxide dismutase, and glucose-6-phosphate in the pathogenesis of rheumatoid arthritis. Clin Rheum 2008;27:141–45.10.1007/s10067-007-0746-3Suche in Google Scholar PubMed
46. Gulçin I. Antioxidant activity of eugenol: a structure-activity relationship study. J Med Food 2011;14:975–85.10.1089/jmf.2010.0197Suche in Google Scholar PubMed
47. Khan MS, Ahmad I. Antibiofilm activity of certain phytocompounds and their synergy with fluconazole against Candida albicans biofilms. J Antimicrob Chemother 2012;67:618–21.10.1093/jac/dkr512Suche in Google Scholar PubMed
©2019 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Oxidative Stress
- Primary concept of nickel toxicity – an overview
- Behavior and Neuroprotection
- Review of thiamine deficiency disorders: Wernicke encephalopathy and Korsakoff psychosis
- Infection
- Opportunistic infection at the start of antiretroviral therapy and baseline CD4+ count less than 50 cells/mm3 are associated with poor immunological recovery
- Inflammation
- Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract
- Oxidative Stress
- Influence of eugenol on oxidative stress biomarkers in the liver of carrageenan-induced arthritis rats
- Selective serotonin reuptake inhibitor fluvoxamine ameliorates stress- and NSAID-induced peptic ulcer possibly by involving Hsp70
- The ethanol leaf extract of Andrographis paniculata blunts acute renal failure in cisplatin-induced injury in rats through inhibition of Kim-1 and upregulation of Nrf2 pathway
- Effect of l-carnitine on aspartame-induced oxidative stress, histopathological changes, and genotoxicity in liver of male rats
- Reproduction
- Sympathovagal imbalance and neurophysiologic cognitive assessment using evoked potentials in polycystic ovary syndrome in young adolescents – a cross-sectional study
- Phytotherapy
- Antidiabetic and antihyperlipidemic potential of ethanol extract of Salacia lehmbachii stem bark in alloxan-induced diabetic rats
- Hypoglycemic and antioxidant effects of oral administration of garlic extract in the livers of type 1 diabetic rats
- Anabasis aretioides Coss. & Moq. phenolic compounds exhibit in vitro hypoglycemic, antioxidant and antipathogenic properties
- Cardiovascular Function
- Cardioautonomic responses to acute ingestion of ice water and its correlation to body mass index
- Metabolism
- Repeated restraint stress upregulates rat sulfotransferase 1A1
- Case Report
- Tramadol-induced parkinsonism: a case report of a 75-year-old woman
Artikel in diesem Heft
- Frontmatter
- Oxidative Stress
- Primary concept of nickel toxicity – an overview
- Behavior and Neuroprotection
- Review of thiamine deficiency disorders: Wernicke encephalopathy and Korsakoff psychosis
- Infection
- Opportunistic infection at the start of antiretroviral therapy and baseline CD4+ count less than 50 cells/mm3 are associated with poor immunological recovery
- Inflammation
- Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract
- Oxidative Stress
- Influence of eugenol on oxidative stress biomarkers in the liver of carrageenan-induced arthritis rats
- Selective serotonin reuptake inhibitor fluvoxamine ameliorates stress- and NSAID-induced peptic ulcer possibly by involving Hsp70
- The ethanol leaf extract of Andrographis paniculata blunts acute renal failure in cisplatin-induced injury in rats through inhibition of Kim-1 and upregulation of Nrf2 pathway
- Effect of l-carnitine on aspartame-induced oxidative stress, histopathological changes, and genotoxicity in liver of male rats
- Reproduction
- Sympathovagal imbalance and neurophysiologic cognitive assessment using evoked potentials in polycystic ovary syndrome in young adolescents – a cross-sectional study
- Phytotherapy
- Antidiabetic and antihyperlipidemic potential of ethanol extract of Salacia lehmbachii stem bark in alloxan-induced diabetic rats
- Hypoglycemic and antioxidant effects of oral administration of garlic extract in the livers of type 1 diabetic rats
- Anabasis aretioides Coss. & Moq. phenolic compounds exhibit in vitro hypoglycemic, antioxidant and antipathogenic properties
- Cardiovascular Function
- Cardioautonomic responses to acute ingestion of ice water and its correlation to body mass index
- Metabolism
- Repeated restraint stress upregulates rat sulfotransferase 1A1
- Case Report
- Tramadol-induced parkinsonism: a case report of a 75-year-old woman
