Startseite Repeated restraint stress upregulates rat sulfotransferase 1A1
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Repeated restraint stress upregulates rat sulfotransferase 1A1

  • Rajiv Balyan , Ma Cai , Wenhong Zhao , Zhao Dai , Yujia Zhai und Guangping Chen EMAIL logo
Veröffentlicht/Copyright: 20. Dezember 2018
Journal of Basic and Clinical Physiology and Pharmacology
Aus der Zeitschrift Journal of Basic and Clinical Physiology and Pharmacology Band 30 Heft 2

Abstract

Background

Sulfotransferases (SULTs) are phase II drug-metabolizing enzymes. SULTs also regulate the biological activities of biological signaling molecules, such as various hormones, bile acids, and monoamine neurotransmitters; therefore, they play critical roles in the endocrine and nervous systems. People are subject to various kinds of physical, chemical, toxicological, physiological, and psychological stresses at one time or another. The study of the effects produced by stress may lead to finding novel remedies for many disease conditions. The effect of repeated restraint stress on rat SULT expression has not been studied.

Methods

This study involves the effect of repeated restraint stress on SULT1A1 expressions. Male Sprague-Dawley rats (n=4) were subjected to repeated restraint stress 2 h/day for 7 days. Protein and RNA expression of SULT1A1 were analyzed by western blot and quantitative real time reverse transcription polymerase chain reaction, respectively, in important tissues.

Results

We observed that repeated restraint stress increased the expression of SULT1A1 in the liver, adrenal glands, cerebellum, hypothalamus, and cerebral cortex in male rats. Patterns of enhanced expression were observed at both mRNA and protein level, indicating that repeated restraint stress stimulates enzyme expression at the transcriptional level.

Conclusions

Changes of SULT1A1 expression in important tissues caused by repeated restraint stress will have a significant effect on drug metabolism and xenobiotics detoxification. The significant changes in endocrine glands and brain sections may also cause disturbances in hormone homeostasis, therefore leading to disease conditions. This report provides clues for the understanding of the effect of stresses on health.

Keywords: drug metabolizing enzymes; gene regulation; hormone homeostasis; hormone regulation; repeated restraint stress; sulfotransferase

Acknowledgments

The authors are grateful to Dr. Michael W. Duffel (The University of Iowa) for the generous gift of anti-rSULT1A1 antibody. This work was supported in part by the National Institutes of Health grant R01GM078606, the American Cancer Society grant RSG-07-028-01-CNE, and the United States Department of Agriculture grant 2006-35200-17137 (G.C.). The authors would like to thank Mrs. Tammy Austin for proofreading the manuscript.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: National Institutes of Health grant R01GM078606, Funder Id: 10.13039/100005440, American Cancer Society grant RSG-07-028-01-CNE, United States Department of Agriculture grant 2006-35200-17137 (G.C.).

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2016-03-19
Accepted: 2018-11-15
Published Online: 2018-12-20

©2019 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Oxidative Stress
  3. Primary concept of nickel toxicity – an overview
  4. Behavior and Neuroprotection
  5. Review of thiamine deficiency disorders: Wernicke encephalopathy and Korsakoff psychosis
  6. Infection
  7. Opportunistic infection at the start of antiretroviral therapy and baseline CD4+ count less than 50 cells/mm3 are associated with poor immunological recovery
  8. Inflammation
  9. Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract
  10. Oxidative Stress
  11. Influence of eugenol on oxidative stress biomarkers in the liver of carrageenan-induced arthritis rats
  12. Selective serotonin reuptake inhibitor fluvoxamine ameliorates stress- and NSAID-induced peptic ulcer possibly by involving Hsp70
  13. The ethanol leaf extract of Andrographis paniculata blunts acute renal failure in cisplatin-induced injury in rats through inhibition of Kim-1 and upregulation of Nrf2 pathway
  14. Effect of l-carnitine on aspartame-induced oxidative stress, histopathological changes, and genotoxicity in liver of male rats
  15. Reproduction
  16. Sympathovagal imbalance and neurophysiologic cognitive assessment using evoked potentials in polycystic ovary syndrome in young adolescents – a cross-sectional study
  17. Phytotherapy
  18. Antidiabetic and antihyperlipidemic potential of ethanol extract of Salacia lehmbachii stem bark in alloxan-induced diabetic rats
  19. Hypoglycemic and antioxidant effects of oral administration of garlic extract in the livers of type 1 diabetic rats
  20. Anabasis aretioides Coss. & Moq. phenolic compounds exhibit in vitro hypoglycemic, antioxidant and antipathogenic properties
  21. Cardiovascular Function
  22. Cardioautonomic responses to acute ingestion of ice water and its correlation to body mass index
  23. Metabolism
  24. Repeated restraint stress upregulates rat sulfotransferase 1A1
  25. Case Report
  26. Tramadol-induced parkinsonism: a case report of a 75-year-old woman
https://doi.org/10.1515/jbcpp-2016-0038
Schlagwörter für diesen Artikel
drug metabolizing enzymes; gene regulation; hormone homeostasis; hormone regulation; repeated restraint stress; sulfotransferase

Artikel in diesem Heft

  1. Frontmatter
  2. Oxidative Stress
  3. Primary concept of nickel toxicity – an overview
  4. Behavior and Neuroprotection
  5. Review of thiamine deficiency disorders: Wernicke encephalopathy and Korsakoff psychosis
  6. Infection
  7. Opportunistic infection at the start of antiretroviral therapy and baseline CD4+ count less than 50 cells/mm3 are associated with poor immunological recovery
  8. Inflammation
  9. Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract
  10. Oxidative Stress
  11. Influence of eugenol on oxidative stress biomarkers in the liver of carrageenan-induced arthritis rats
  12. Selective serotonin reuptake inhibitor fluvoxamine ameliorates stress- and NSAID-induced peptic ulcer possibly by involving Hsp70
  13. The ethanol leaf extract of Andrographis paniculata blunts acute renal failure in cisplatin-induced injury in rats through inhibition of Kim-1 and upregulation of Nrf2 pathway
  14. Effect of l-carnitine on aspartame-induced oxidative stress, histopathological changes, and genotoxicity in liver of male rats
  15. Reproduction
  16. Sympathovagal imbalance and neurophysiologic cognitive assessment using evoked potentials in polycystic ovary syndrome in young adolescents – a cross-sectional study
  17. Phytotherapy
  18. Antidiabetic and antihyperlipidemic potential of ethanol extract of Salacia lehmbachii stem bark in alloxan-induced diabetic rats
  19. Hypoglycemic and antioxidant effects of oral administration of garlic extract in the livers of type 1 diabetic rats
  20. Anabasis aretioides Coss. & Moq. phenolic compounds exhibit in vitro hypoglycemic, antioxidant and antipathogenic properties
  21. Cardiovascular Function
  22. Cardioautonomic responses to acute ingestion of ice water and its correlation to body mass index
  23. Metabolism
  24. Repeated restraint stress upregulates rat sulfotransferase 1A1
  25. Case Report
  26. Tramadol-induced parkinsonism: a case report of a 75-year-old woman
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