Phytochemical, sub-acute toxicity, and antibacterial evaluation of Cordia sebestena leaf extracts
-
Adeleke Osho
,
Charles B. Adeosun
Abstract
Background: In Nigeria, Cordia sebestena (Boraginaceae), an understudied medicinal plant, is used in traditional medicine for the treatment of gastrointestinal disorders. In this study, we investigated the chemical composition, antibacterial potential, and sub-acute toxicity of C. sebestena leaves.
Methods: Ethyl acetate extracts were analyzed using thin layer chromatography (TLC) and Fourier transform infrared (FTIR) spectrophotometry. The antibacterial potential of the extracts was tested against five standard bacteria, namely Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Clinical observations and blood parameters were used to evaluate the possible toxicity of C. sebestena.
Results: The TLC profile yielded 39 fractions, which were pooled to nine combined sub-fractions (A–I). The FTIR spectrum of sub-fraction H indicated the presence of aliphatic C-H stretching vibration at 2922 and 2850 cm−1, C=O stretch at 1734 and 1708 cm−1, and C=C stretch of aromatics and aliphatics at 1464 and (shoulder) 1618 cm−1, respectively. The fractions of the C. sebestena ethyl acetate leaf extract showed antibacterial potential across board, but fraction H had the highest antibacterial activity against B. cereus and S. aureus. The study also indicated the relatively low toxicity profile of the ethyl acetate leaf extract of C. sebestena in the liver of rats.
Conclusions: The study showed that C. sebestena leaves have strong antibacterial potential and low toxicity, thereby underlying the scientific basis for their folkloric use in the management of microbial infections and its associated complications.
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Articles in the same Issue
- Frontmatter
- Review
- Ulinastatin – a newer potential therapeutic option for multiple organ dysfunction syndrome
- Behavior and Neuroprotection
- Amitriptyline and phenytoin prevents memory deficit in sciatic nerve ligation model of neuropathic pain
- Anti-nociceptive activity of a few structurally related trimethoxy flavones and possible mechanisms involved
- Oxidative Stress
- A mechanism-based pharmacological evaluation of efficacy of Flacourtia indica in management of dyslipidemia and oxidative stress in hyperlipidemic rats
- Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro
- Biochemical effects of lead exposure on oxidative stress and antioxidant status of battery manufacturing workers of Western Maharashtra, India
- Hematological Profile
- Phenotypic variations in osmotic lysis of Sahel goat erythrocytes in non-ionic glucose media
- Infection
- Prevalence, risk factors and antimicrobial susceptibility pattern of extended spectrum β-lactamase-producing bacteria in a tertiary care hospital
- Phytotherapy
- Phytochemical, sub-acute toxicity, and antibacterial evaluation of Cordia sebestena leaf extracts
- Short Communication
- Leaching from the stratum corneum does not explain the previously reported elevated potassium ion concentration in sweat
Articles in the same Issue
- Frontmatter
- Review
- Ulinastatin – a newer potential therapeutic option for multiple organ dysfunction syndrome
- Behavior and Neuroprotection
- Amitriptyline and phenytoin prevents memory deficit in sciatic nerve ligation model of neuropathic pain
- Anti-nociceptive activity of a few structurally related trimethoxy flavones and possible mechanisms involved
- Oxidative Stress
- A mechanism-based pharmacological evaluation of efficacy of Flacourtia indica in management of dyslipidemia and oxidative stress in hyperlipidemic rats
- Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro
- Biochemical effects of lead exposure on oxidative stress and antioxidant status of battery manufacturing workers of Western Maharashtra, India
- Hematological Profile
- Phenotypic variations in osmotic lysis of Sahel goat erythrocytes in non-ionic glucose media
- Infection
- Prevalence, risk factors and antimicrobial susceptibility pattern of extended spectrum β-lactamase-producing bacteria in a tertiary care hospital
- Phytotherapy
- Phytochemical, sub-acute toxicity, and antibacterial evaluation of Cordia sebestena leaf extracts
- Short Communication
- Leaching from the stratum corneum does not explain the previously reported elevated potassium ion concentration in sweat
