Phenotypic variations in osmotic lysis of Sahel goat erythrocytes in non-ionic glucose media
-
Nanacha Afifi Igbokwe
and
Ikechukwu Onyebuchi Igbokwe
Abstract
Background: Erythrocyte osmotic lysis in deionised glucose media is regulated by glucose influx, cation efflux, and changes in cell volume after water diffusion. Transmembrane fluxes may be affected by varied expression of glucose transporter protein and susceptibility of membrane proteins to glucose-induced glycosylation and oxidation in various physiologic states.
Methods: Variations in haemolysis of Sahel goat erythrocytes after incubation in hyposmotic non-ionic glucose media, associated with sex, age, late pregnancy, and lactation, were investigated.
Results: The osmotic fragility curve in glucose media was sigmoidal with erythrocytes from goats in late pregnancy (PRE) or lactation (LAC) or from kid (KGT) or middle-aged (MGT) goats. Non-sigmoidal phenotype occurred in yearlings (YGT) and old (OGT) goats. The composite fragility phenotype for males and non-pregnant dry (NPD) females was non-sigmoidal. Erythrocytes with non-sigmoidal curves were more stable than those with sigmoidal curves because of inflectional shift of the curve to the left. Erythrocytes tended to be more fragile with male than female sex, KGT and MGT than YGT and OGT, and LAC and PRE than NPD. Thus, sex, age, pregnancy, and lactation affected the haemolytic pattern of goat erythrocytes in glucose media.
Conclusions: The physiologic state of the goat affected the in vitro interaction of glucose with erythrocytes, causing variations in osmotic stability with variants of fragility phenotype. Variations in the effect of high extracellular glucose concentrations on the functions of membrane-associated glucose transporter, aquaporins, and the cation cotransporter were presumed to be relevant in regulating the physical properties of goat erythrocytes under osmotic stress.
Acknowledgments
Ismaila Gadaka, Chima V. Maduka, Alakpa B. Ikpe, and Sylvester Ogbaji provided technical and material assistance.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Review
- Ulinastatin – a newer potential therapeutic option for multiple organ dysfunction syndrome
- Behavior and Neuroprotection
- Amitriptyline and phenytoin prevents memory deficit in sciatic nerve ligation model of neuropathic pain
- Anti-nociceptive activity of a few structurally related trimethoxy flavones and possible mechanisms involved
- Oxidative Stress
- A mechanism-based pharmacological evaluation of efficacy of Flacourtia indica in management of dyslipidemia and oxidative stress in hyperlipidemic rats
- Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro
- Biochemical effects of lead exposure on oxidative stress and antioxidant status of battery manufacturing workers of Western Maharashtra, India
- Hematological Profile
- Phenotypic variations in osmotic lysis of Sahel goat erythrocytes in non-ionic glucose media
- Infection
- Prevalence, risk factors and antimicrobial susceptibility pattern of extended spectrum β-lactamase-producing bacteria in a tertiary care hospital
- Phytotherapy
- Phytochemical, sub-acute toxicity, and antibacterial evaluation of Cordia sebestena leaf extracts
- Short Communication
- Leaching from the stratum corneum does not explain the previously reported elevated potassium ion concentration in sweat
Articles in the same Issue
- Frontmatter
- Review
- Ulinastatin – a newer potential therapeutic option for multiple organ dysfunction syndrome
- Behavior and Neuroprotection
- Amitriptyline and phenytoin prevents memory deficit in sciatic nerve ligation model of neuropathic pain
- Anti-nociceptive activity of a few structurally related trimethoxy flavones and possible mechanisms involved
- Oxidative Stress
- A mechanism-based pharmacological evaluation of efficacy of Flacourtia indica in management of dyslipidemia and oxidative stress in hyperlipidemic rats
- Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro
- Biochemical effects of lead exposure on oxidative stress and antioxidant status of battery manufacturing workers of Western Maharashtra, India
- Hematological Profile
- Phenotypic variations in osmotic lysis of Sahel goat erythrocytes in non-ionic glucose media
- Infection
- Prevalence, risk factors and antimicrobial susceptibility pattern of extended spectrum β-lactamase-producing bacteria in a tertiary care hospital
- Phytotherapy
- Phytochemical, sub-acute toxicity, and antibacterial evaluation of Cordia sebestena leaf extracts
- Short Communication
- Leaching from the stratum corneum does not explain the previously reported elevated potassium ion concentration in sweat
