Amitriptyline and phenytoin prevents memory deficit in sciatic nerve ligation model of neuropathic pain
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Wahab Imam Abdulmajeed
Abstract
Background: Phenytoin and amitriptyline are often reported to attenuate pain in chronic conditions. Information on their ability to ameliorate cognitive impairment associated with neuropathic pain remains unclear due to mixed results from studies. This study investigated the effects of phenytoin and amitriptyline on memory deficit associated with neuropathic pain.
Methods: Twenty-eight adult male Wistar rats were randomly divided into four groups: A, B, C, and D (n=7). Groups A, B, C, and D served as sham control, sciatic nerve ligated untreated, sciatic nerve ligated receiving amitriptyline (5 mg/kg), and sciatic nerve ligated receiving phenytoin (10 mg/kg) respectively. Treatments lasted for 14 days, after which both ‘Y’ maze and novel object recognition test (NOR) were performed. On the last day of treatment, the animals were anesthetized and their brain excised, and the prefrontal cortices and sciatic nerve were processed histologically using hematoxylin and eosin.
Results: There was memory impairment in the sciatic nerve ligated untreated group which was statistically significant (p<0.05) when compared to the phenytoin-treated, amitriptyline-treated, and sham control groups using the ‘Y’ maze and NOR tests. Histological quantification showed that the prefrontal cortices of the ligated animals showed increased neural population in comparison to normal control. These increases were significantly marked in the untreated ligated group. Sciatic nerve of untreated ligated group showed high demyelination and axonal degeneration which was ameliorated in the treated animals.
Conclusions: The administration of amitriptyline and phenytoin can ameliorate neuronal injury, demyelination, and memory impairment associated with neuropathic pain in Wistar rats.
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Review
- Ulinastatin – a newer potential therapeutic option for multiple organ dysfunction syndrome
- Behavior and Neuroprotection
- Amitriptyline and phenytoin prevents memory deficit in sciatic nerve ligation model of neuropathic pain
- Anti-nociceptive activity of a few structurally related trimethoxy flavones and possible mechanisms involved
- Oxidative Stress
- A mechanism-based pharmacological evaluation of efficacy of Flacourtia indica in management of dyslipidemia and oxidative stress in hyperlipidemic rats
- Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro
- Biochemical effects of lead exposure on oxidative stress and antioxidant status of battery manufacturing workers of Western Maharashtra, India
- Hematological Profile
- Phenotypic variations in osmotic lysis of Sahel goat erythrocytes in non-ionic glucose media
- Infection
- Prevalence, risk factors and antimicrobial susceptibility pattern of extended spectrum β-lactamase-producing bacteria in a tertiary care hospital
- Phytotherapy
- Phytochemical, sub-acute toxicity, and antibacterial evaluation of Cordia sebestena leaf extracts
- Short Communication
- Leaching from the stratum corneum does not explain the previously reported elevated potassium ion concentration in sweat
Articles in the same Issue
- Frontmatter
- Review
- Ulinastatin – a newer potential therapeutic option for multiple organ dysfunction syndrome
- Behavior and Neuroprotection
- Amitriptyline and phenytoin prevents memory deficit in sciatic nerve ligation model of neuropathic pain
- Anti-nociceptive activity of a few structurally related trimethoxy flavones and possible mechanisms involved
- Oxidative Stress
- A mechanism-based pharmacological evaluation of efficacy of Flacourtia indica in management of dyslipidemia and oxidative stress in hyperlipidemic rats
- Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro
- Biochemical effects of lead exposure on oxidative stress and antioxidant status of battery manufacturing workers of Western Maharashtra, India
- Hematological Profile
- Phenotypic variations in osmotic lysis of Sahel goat erythrocytes in non-ionic glucose media
- Infection
- Prevalence, risk factors and antimicrobial susceptibility pattern of extended spectrum β-lactamase-producing bacteria in a tertiary care hospital
- Phytotherapy
- Phytochemical, sub-acute toxicity, and antibacterial evaluation of Cordia sebestena leaf extracts
- Short Communication
- Leaching from the stratum corneum does not explain the previously reported elevated potassium ion concentration in sweat