Sensitive and selective determination of peptides, PG and PGP, using a novel fluorogenic reagent 4-chlorobenzene-1,2-diol
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Hasina Yasmin
,
Takayuki Shibata
Abstract
A novel fluorometric method was developed for the sensitive and selective detection of Pro-Gly (PG) and Pro-Gly-Pro (PGP) using 4-chlorobenzene-1,2-diol (4-CBD) as a fluorogenic reagent. The reaction was performed at 37°C for 30 min in the presence of a borate buffer (pH 7.0) and sodium periodate. The resulting fluorescence intensity was measured using a spectrofluorometer with excitation and emission wavelengths of 450 nm and 535 nm. To obtain a stable fluorescent signal and maximise its intensity, different reaction conditions such as the concentrations of the reagents, the reaction time, and the pH were optimised. Under the optimised conditions, a linear relationship was obtained between fluorescence intensity and peptide concentrations from 1.0-40.0 μmol L-1 with a limit of detection of 1.0 μmol L-1 (S/N = 3). Both PG and PGP generated a strong signal out of all the peptides tested and no other biogenic substances such as amino acids or proteins produced any fluorescence. The reaction thus developed is simple, rapid, selective, and sensitive. It can be applied to the determination of peptides as biomarkers or substrates.
References
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© 2015 Institute of Chemistry, Slovak Academy of Sciences
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Artikel in diesem Heft
- Liquid–liquid extraction and cloud point extraction for spectrophotometric determination of vanadium using 4-(2-pyridylazo)resorcinol
- Sensitive and selective determination of peptides, PG and PGP, using a novel fluorogenic reagent 4-chlorobenzene-1,2-diol
- Spectroscopy studies of sandwich-type complex of silver(I) co-ordinated to nuclear fast red and adenine and its analytical applications
- Differentiation of selected blue writing inks by surface-enhanced Raman spectroscopy
- A simple pyridine-based colorimetric chemosensor for highly sensitive and selective mercury(II) detection with the naked eye
- Phospho sulfonic acid as efficient heterogeneous Brønsted acidic catalyst for one-pot synthesis of 14H-dibenzo[a,j ]xanthenes and 1,8-dioxo-octahydro-xanthenes
- Microfiltration of post-fermentation broth with backflushing membrane cleaning
- Mass transfer examination in electrodialysis using limiting current measurements
- Determination of diffusivity from mass transfer measurements in a batch dialyzer: numerical analysis of pseudo-steady state approximation
- Structural and thermal characterization of copper(II) complexes with phenyl-2-pyridylketoxime and deposition of thin films by spin coating
- Oxidation of 4-nitro-o-xylene with nitric acid using N-hydroxyphthalimide under phase transfer conditions
- Synthesis of pyranopyrazoles, benzopyrans, amino-2-chromenes and dihydropyrano[c]chromenes using ionic liquid with dual Brønsted acidic and Lewis basic sites
- Eco-friendly conjugate hydrocyanation of α-cyanoacrylates using potassium hexacyanoferrate(II) as cyanating reagent
- Morphological orders of spherulitic crystal textures in Belousov–Zhabotinsky-type oscillatory reaction system
- Zwitterionic structures of selenocysteine-containing dipeptides and their interactions with Cu(II) ions
