Detection of the antibacterial effect of Chaetomium cochliodes Palliser CCM F-232 based on agar plugs and unprocessed fungal substances from cultivation media
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Katarína Chovanová
und
Marcel Zámocký
Abstract
There is an increasing demand for novel antibiotics that are highly effective, but simultaneously reveal minimal side effects. Currently, major sources of antibiotics are found among bacterial genus Streptomyces and among fungal genera Penicillium and Aspergillus. Chaetomium represents the largest genus of the family Chaetomiaceae actually containing more than 100 described species. This rather complex genus has been reported as a rich source of secondary metabolite products with important biological activities, such as enzyme-inhibitory, antitumour, cytotoxic, antimalarial and also antibiotic effects. One of them, chaetomin, a part of Chaetomium cochliodes antimicrobial equipment was discovered already in the early period of antibiotics discovery, but later not investigated in sufficient detail. In this contribution we have studied Chaetomium cochliodes Palliser CCM F-232 for its possible antibacterial properties. For comparisons, a closely related fungus Chaetomium globosum was also employed. Antibacterial properties of Chaetomium sp. were tested on natural bacterial isolates of Staphylococcus aureus and Bacillus subtilis. We performed several antimicrobial tests with a correlating output. Antibacterial effects of these filamentous fungi were demonstrated either by the presence of susceptibility zones on solid agar media in the case of S. aureus or by growth inhibition in liquid broth in the case of Staphylococcus aureus and Bacillus subtilis.
Acknowledgements
Our research was supported by the Slovak Research and Development Agency with grant APVV-14-0375 and by the Slovak Grant Agency VEGA with grant 2/0021/14.
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- Abbreviations
- A600
absorbance measured at a wavelength of 600 nm
- CD agar or broth
Czapek Dox agar or broth with 1% corn extract powder
- IM
intermediate
- LB
Luria-Bertani
- MPG agar or broth
malt peptone glucose agar or broth
- PME agar or broth
potato malt extract agar or broth with stripes of filter paper
- RT
room temperature 0.45 ìmCco/Cgl
- MPG/PME/CD
filtrates through 0.45 ìm membrane obtained from C. cochliodes or C. globosum cultivated in MPG, PME or CD broth.
© 2016 Institute of Molecular Biology, Slovak Academy of Sciences
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Artikel in diesem Heft
- Cellular and Molecular Biology
- The evolutionary pathway of the staphylococcal cassette chromosome element
- Cellular and Molecular Biology
- Detection of the antibacterial effect of Chaetomium cochliodes Palliser CCM F-232 based on agar plugs and unprocessed fungal substances from cultivation media
- Botany
- Identification and molecular characterization of one novel 1Sl-encoded s-type low molecular weight glutenin B-subunit from 1Sl(1B) substitution line of wheat variety Chinese Spring (Triticum aestivum)
- Botany
- Bioinformatic analysis of Arabidopsis reverse transcriptases with a zinc-finger domain
- Botany
- Distinct expression patterns of the GDP dissociation inhibitor protein gene (OsRhoGDI2) from Oryza sativa during development and abiotic stresses
- Botany
- An application of genetics-chemicals constituents to the relatedness of three Euphorbia species
- Zoology
- Centipede (Chilopoda) richness, diversity and community structure in the forest-steppe nature reserve “Bielinek” on the Odra River (NW Poland, Central Europe)
- Zoology
- Genetic differentiating Aphis fabae and Aphis craccivora (Hemiptera: Sternorranycha: Aphididae) populations in Egypt using mitochondrial COI
- Zoology
- A faunistic study on Carabidae and Scarabaeidae in alfalfa fields from Central Greece
- Zoology
- Comparison of Macroheterocera assemblages of four forests in the Bereg Plain (Hungary, Ukraine)
- Zoology
- Late flooding combined with warm autumn – potential possibility for prolongation of transmission of mosquito-borne diseases
- Zoology
- Temperature and precipitation effects on breeding productivity of some passerines – a multivariate analysis of constant effort mist-netting data
- Cellular and Molecular Biology
- The direct action of hyaluronic acid on human U-937 and HL-60 cells – modification of native and model membranes
