Detection of the antibacterial effect of Chaetomium cochliodes Palliser CCM F-232 based on agar plugs and unprocessed fungal substances from cultivation media
-
Katarína Chovanová
and
Marcel Zámocký
Abstract
There is an increasing demand for novel antibiotics that are highly effective, but simultaneously reveal minimal side effects. Currently, major sources of antibiotics are found among bacterial genus Streptomyces and among fungal genera Penicillium and Aspergillus. Chaetomium represents the largest genus of the family Chaetomiaceae actually containing more than 100 described species. This rather complex genus has been reported as a rich source of secondary metabolite products with important biological activities, such as enzyme-inhibitory, antitumour, cytotoxic, antimalarial and also antibiotic effects. One of them, chaetomin, a part of Chaetomium cochliodes antimicrobial equipment was discovered already in the early period of antibiotics discovery, but later not investigated in sufficient detail. In this contribution we have studied Chaetomium cochliodes Palliser CCM F-232 for its possible antibacterial properties. For comparisons, a closely related fungus Chaetomium globosum was also employed. Antibacterial properties of Chaetomium sp. were tested on natural bacterial isolates of Staphylococcus aureus and Bacillus subtilis. We performed several antimicrobial tests with a correlating output. Antibacterial effects of these filamentous fungi were demonstrated either by the presence of susceptibility zones on solid agar media in the case of S. aureus or by growth inhibition in liquid broth in the case of Staphylococcus aureus and Bacillus subtilis.
Acknowledgements
Our research was supported by the Slovak Research and Development Agency with grant APVV-14-0375 and by the Slovak Grant Agency VEGA with grant 2/0021/14.
References
Bai H., Wu L., Yang T. & Li G. 2015. Isolation and identification of secondary metabolites from fungus Chaetomium gracile and their antimicrobial activities. Chin. J. Appl. Environ. Biol. 21: 274–278.Search in Google Scholar
Berdy J. 2012. Thoughts and facts about antibiotics: where we are now and where we are heading. J. Antibiot. 65: 385–395.10.1038/ja.2012.27Search in Google Scholar PubMed
Deng Y., Hua Z., Zhao Z. & Chen J. 2008. Effect of nitrogen sources on catalase production by Bacillus subtilis WSHDZ-01. Chin. J. Appl. Environ. Biol. 14: 544–547.Search in Google Scholar
de Siqueira V.M., de Araujo R.J.M. & Souza-Motta C.M. 2011. Endophytic fungi from the medicinal plant Lippia sidoides Cham. and their antimicrobial activity. Symbiosis 53: 89–95.10.1007/s13199-011-0113-7Search in Google Scholar
Frisvad J. C. 1986. Taxonomic approaches to mycotoxin identification, pp. 415–457. In: Cole R.J. (ed.) Modern Methods in the Analysis and Structural Elucidation of Mycotoxins. Academic Press, Inc., New York.10.1016/B978-0-12-179515-3.50018-5Search in Google Scholar
Garcia-Kirchner O., Segura-Granados M., Robledo-Bautista I. & Duran-Paramo E. 2000. Screening of potential antibiotic action of cellulolytic fungi. Appl. Biochem. Biotechnol. 84-86 769–778.10.1007/978-1-4612-1392-5_60Search in Google Scholar
Geiger W.B., Conn J.E. & Waksman S.A. 1944. Chaetomin, a new antibiotic substance produced by Chaetomium cochliodes. II. Isolation and concentration. J. Bacteriol. 48: 531–536.10.1128/jb.48.5.531-536.1944Search in Google Scholar PubMed PubMed Central
Hutchinson M.I., Powell A.J., Tsang A., O’Toole N., Berka R.M., Barry K., Grigoriev I.V. & Natvig D.O. 2016. Genetics of mating in members of the Chaetomiaceae as revealed by experimental and genomic characterization of reproduction in Myceliophthora heterothallica. Fungal Genet. Biol. 86: 9–19.10.1016/j.fgb.2015.11.007Search in Google Scholar PubMed
Hwang E.I., Yun B.S., Kim Y.K., Kwon B.M., Kim H.G., Lee H.B. & Kim S U. 2000. Chaetoatrosin A, a novel chitin synthase II inhibitor produced by Chaetomium atrobrunneum F449. J. Antibiot. 53: 248–255.10.7164/antibiotics.53.248Search in Google Scholar PubMed
Kobayashi M., Yoshimura S., Kinoshita T., Hashimoto M., Hashimoto S., Takase S., Fujie A., Hino M. & Hori Y. 2005. FR207944, an antifungal antibiotic from Chaetomium sp. no. 217 II. Isolation and structure elucidation. Biosci. Biotechnol. Biochem. 69: 1029–1032.10.1271/bbb.69.1029Search in Google Scholar PubMed
Kogel K.H., Franken P. & Hückelhoven R. 2006. Endophyte or parasite – what decides? Curr. Opin. Plant Biol. 9: 358–363.10.1016/j.pbi.2006.05.001Search in Google Scholar PubMed
Lam K.S. 2007. New aspects of natural products in drug discovery. Trends Microbiol. 15: 279–289.10.1016/j.tim.2007.04.001Search in Google Scholar PubMed
Nielsen K.F., Gravesen S., Nielsen P.A., Andersen B., Thrane U. & Frisvad J.C. 1999. Production of mycotoxins on artificially and naturally infested building materials. Mycopathol. 145: 43–56.10.1023/A:1007038211176Search in Google Scholar
Oh H., Swenson D.C. & Gloer J.B. 1998. Chaetochalasin A: a new bioactive metabolite from Chaetomium brasiliense. Tetrahedron Lett. 39: 7633-7636.10.1016/S0040-4039(98)01692-XSearch in Google Scholar
Pereira E., Santos A., Reis F., Tavares R.M., Baptista P., Lino-Neto T. & Almeida-Aguiar C. 2013. A new effective assay to detect antimicrobial activity of filamentous fungi. Microbiol. Res. 168: 1–5.10.1016/j.micres.2012.06.008Search in Google Scholar
Pettit R.K. 2011. Small–molecule elicitation of microbial secondary metabolites.Microb. Biotechnol. 4: 471–478.10.1111/j.1751-7915.2010.00196.xSearch in Google Scholar
Pitt J.I. & Samson R.A. 1990. Approaches to Penicillium and Aspergillus systematics. Stud. Mycol. 32: 77-90.Search in Google Scholar
Piyasena K.G.N.P., Wickramarachchi W.A.R.T., Savitri Kumar N., Jayasinghe L. & Fujimoto Y. 2015. Two phytotoxic azaphilone derivatives from Chaetomium globosum, a fungal endophyte isolated from Amaranthus viridis leaves. Mycology 6: 158–160.10.1080/21501203.2015.1089332Search in Google Scholar
Provost N.B., Shi C., She Y.M., Cyr T.D. & Miller J.D. 2013 Characterization of an antigenic chitosanase from the cellulolytic fungus Chaetomium globosum. Med. Mycol. 51: 290–299.10.3109/13693786.2012.715246Search in Google Scholar
Radic N. & Srukelj B. 2012. Endophytic fungi – the treasure chest of antibacterial substances. Phytomedicine 19: 1270–1284.10.1016/j.phymed.2012.09.007Search in Google Scholar
Smith K.M., Gautschi J.T. & Freitag M. 2014. Decoding the cryptic genomes of fungi: the promise of novel antibiotics. Future Microbiol. 9: 265–268.10.2217/fmb.14.6Search in Google Scholar
Song J.H. 2008. What’s new on the antimicrobial horizon? Int. J. Antimicrob. Agents 32: S207–S213.10.1016/S0924-8579(09)70004-4Search in Google Scholar
Theuretzbacher U., Van Bambeke F., Cantón R., Giske C.G., Mouton J.W., Nation R.L., Paul M., Turnidge J.D. & Kahlmeter G. 2015. Reviving old antibiotics. J. Antimicrob. Chemother. 70: 2177–2181.10.1093/jac/dkv157Search in Google Scholar PubMed
Zhang Q., Li H.Q., Zong S.C., Gao J.M. & Zhang A.L. 2012. Chemical and bioactive diversities of the genus Chaetomium secondary metabolites. Mini Rev. Med. Chem. 12: 127–148.10.2174/138955712798995066Search in Google Scholar PubMed
- Abbreviations
- A600
absorbance measured at a wavelength of 600 nm
- CD agar or broth
Czapek Dox agar or broth with 1% corn extract powder
- IM
intermediate
- LB
Luria-Bertani
- MPG agar or broth
malt peptone glucose agar or broth
- PME agar or broth
potato malt extract agar or broth with stripes of filter paper
- RT
room temperature 0.45 ìmCco/Cgl
- MPG/PME/CD
filtrates through 0.45 ìm membrane obtained from C. cochliodes or C. globosum cultivated in MPG, PME or CD broth.
© 2016 Institute of Molecular Biology, Slovak Academy of Sciences
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- Cellular and Molecular Biology
- The evolutionary pathway of the staphylococcal cassette chromosome element
- Cellular and Molecular Biology
- Detection of the antibacterial effect of Chaetomium cochliodes Palliser CCM F-232 based on agar plugs and unprocessed fungal substances from cultivation media
- Botany
- Identification and molecular characterization of one novel 1Sl-encoded s-type low molecular weight glutenin B-subunit from 1Sl(1B) substitution line of wheat variety Chinese Spring (Triticum aestivum)
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- Distinct expression patterns of the GDP dissociation inhibitor protein gene (OsRhoGDI2) from Oryza sativa during development and abiotic stresses
- Botany
- An application of genetics-chemicals constituents to the relatedness of three Euphorbia species
- Zoology
- Centipede (Chilopoda) richness, diversity and community structure in the forest-steppe nature reserve “Bielinek” on the Odra River (NW Poland, Central Europe)
- Zoology
- Genetic differentiating Aphis fabae and Aphis craccivora (Hemiptera: Sternorranycha: Aphididae) populations in Egypt using mitochondrial COI
- Zoology
- A faunistic study on Carabidae and Scarabaeidae in alfalfa fields from Central Greece
- Zoology
- Comparison of Macroheterocera assemblages of four forests in the Bereg Plain (Hungary, Ukraine)
- Zoology
- Late flooding combined with warm autumn – potential possibility for prolongation of transmission of mosquito-borne diseases
- Zoology
- Temperature and precipitation effects on breeding productivity of some passerines – a multivariate analysis of constant effort mist-netting data
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