Abstract
Genetics and chemicals relatedness among three Euphorbia species (Euphorbia prostrata Aiton, Euphorbia peplus L. and Euphorbia terracina L.) were analysed by RAPD, ISSR markers and GC-MS technique. RAPD profiling pattern revealed that E. peplus and E. terracina in one cluster with similarity value (44.37%) while the lowest value (29.14%) between E. prostrata and E. terracina. ISSR profiling pattern revealed that E. prostrata and E. terracina in one cluster with similarity value (46.75%) and the lowest similarity value (37.87%) between E. prostrata and E.peplus. The sum results of RAPD and ISSR data revealed that E. peplus and E. terracina in one cluster with similarity value (44.69%) while the lowest value (36.88%) between E. prostrata and E. peplus. Sixty-eight chemicals pattern revealed that E. prostrata and E. peplus in one cluster with similarity value (39.71%) while the lowest value (25.0%) between E. prostrata and E. terracina. Super tree (RAPD, ISSR and phytocomponents) revealed that E. peplus and E. terracina in one cluster with similarity value (43.04%) while the lowest value (36.08%) between E. terracina and E. prostrata. In conclusion, biosystematics tools including biomarkers and chemical compounds can be applied to investigate relatedness of Euphorbia species and probably to other plant taxa.
- Abbreviations
- RAPD
Random amplified polymorphic DNA
- ISSR
Inter-simple sequence repeats markers
- GC-MS
Gas chromatography-mass spectrometry
Acknowledgements
The authors are grateful to the King Abdulaziz City for Science and Technology (KACST) for the financial support of this work under grant number (A-I-34-280).
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© 2016 Institute of Botany, Slovak Academy of Sciences
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