Startseite An application of genetics-chemicals constituents to the relatedness of three Euphorbia species
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An application of genetics-chemicals constituents to the relatedness of three Euphorbia species

  • Mahmoud Moustafa EMAIL logo , Osama Mostafa , Dema Al-Shahrani und Sulaiman Alrumman
Veröffentlicht/Copyright: 25. Dezember 2016
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Biologia
Aus der Zeitschrift Biologia Band 71 Heft 11

Abstract

Genetics and chemicals relatedness among three Euphorbia species (Euphorbia prostrata Aiton, Euphorbia peplus L. and Euphorbia terracina L.) were analysed by RAPD, ISSR markers and GC-MS technique. RAPD profiling pattern revealed that E. peplus and E. terracina in one cluster with similarity value (44.37%) while the lowest value (29.14%) between E. prostrata and E. terracina. ISSR profiling pattern revealed that E. prostrata and E. terracina in one cluster with similarity value (46.75%) and the lowest similarity value (37.87%) between E. prostrata and E.peplus. The sum results of RAPD and ISSR data revealed that E. peplus and E. terracina in one cluster with similarity value (44.69%) while the lowest value (36.88%) between E. prostrata and E. peplus. Sixty-eight chemicals pattern revealed that E. prostrata and E. peplus in one cluster with similarity value (39.71%) while the lowest value (25.0%) between E. prostrata and E. terracina. Super tree (RAPD, ISSR and phytocomponents) revealed that E. peplus and E. terracina in one cluster with similarity value (43.04%) while the lowest value (36.08%) between E. terracina and E. prostrata. In conclusion, biosystematics tools including biomarkers and chemical compounds can be applied to investigate relatedness of Euphorbia species and probably to other plant taxa.

Abbreviations
RAPD

Random amplified polymorphic DNA

ISSR

Inter-simple sequence repeats markers

GC-MS

Gas chromatography-mass spectrometry

Acknowledgements

The authors are grateful to the King Abdulaziz City for Science and Technology (KACST) for the financial support of this work under grant number (A-I-34-280).

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Received: 2016-5-26
Accepted: 2016-8-10
Published Online: 2016-12-25
Published in Print: 2016-11-1

© 2016 Institute of Botany, Slovak Academy of Sciences

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