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Antimicrobial and morphogenic effects of emodin produced by A spergillus awamori WAIR120

  • Ahmed A. Ismaiel EMAIL logo , Gamal H. Rabie and Marwa A. Abd El-Aal
Published/Copyright: June 16, 2016
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Biologia
From the journal Biologia Volume 71 Issue 5

Abstract

The antimicrobial activity of anthraquinone emodin isolated from Aspergillus awamori WAIR120 (LC032125) culture was investigated against some clinical, phytopathogenic and foodborne pathogenic microorganisms using an agar diffusion method. Among bacterial and fungal strains tested, the highest activity was obtained against Enterococcus faecalis AHR7 as well as Aspergillus niger OC20 with minimal inhibitory emodin concentration of 125 and 85 μg/mL, respectively. Emodin was found to induce morphogenic effects including swelling and elongation of bacterial cell and conidiation decrease, pigmentation loss, and cytoplasmic retraction of fungal cell, as was shown by light microscopy. Additionally, cellular effects were also resulted, in which emodin caused considerable changes in the nature of cell membrane and submicroscopic structure of bacterial and fungal cell, as was shown by transmission electron microscopy. Furthermore, there was an evidence of a disruption of lipid metabolism of fungal cell. These findings thus indicate the future possibility of exploiting emodin as an effective inhibitor of clinical, phytopathogenic and foodborne pathogenic microorganisms.

Key words: emodin; antibacterial; antifungal; ultrastructure; Aspergillus niger; Enterococcus faecalis

Acknowledgements

The authors thank Dr. Basma Hamdy Amin, TEM Unit, Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt, for excellent technical assistance during the processing steps of TEM. The work employed herein was supported in part by the Department of Botany and Microbiology, Faculty of Science, Zagazig University, Egypt.

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Abbreviations
HPLC

high performance liquid chromatography

MIC

minimal inhibitory concentration

TEM

transmission electron microscopy

TLC

thin layer chromatography.

Received: 2016-1-6
Accepted: 2016-5-9
Published Online: 2016-6-16
Published in Print: 2016-5-1

© 2016 Institute of Botany, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2016-0067
Keywords for this article
emodin; antibacterial; antifungal; ultrastructure; Aspergillus niger; Enterococcus faecalis

Articles in the same Issue

  1. Cellular and Molecular Biology
  2. A broad host range food-grade cloning vector for lactic acid bacteria
  3. Cellular and Molecular Biology
  4. Antimicrobial and morphogenic effects of emodin produced by A spergillus awamori WAIR120
  5. Cellular and Molecular Biology
  6. Herbal augmentation enhances malachite green bio degradation efficacy of Saccharomyces cerevisiae
  7. Cellular and Molecular Biology
  8. Efflux pump inhibitory activity of flavonoids isolated from Alpinia calcarata against methicillin-resistant Staphylococcus aureus
  9. Cellular and Molecular Biology
  10. Status of metal pollution in rivers flowing through urban settlements at Pune and its effect on resident microflora
  11. Cellular and Molecular Biology
  12. Use of N, N′-diacetylchitobiose in decreasing toxic effects of indoor air pollution by preventing oxidative DNA damage
  13. Botany
  14. The relationship between macrophyte assemblages and environmental variables in drainage and irrigation canals in Slovakia
  15. Botany
  16. Effects of ZnCl2 on ROS generation, plasma membrane properties, and changes in protein expression in grapevine root explants
  17. Botany
  18. Molecular characterization and alternative splicing of a MYB transcription factor gene in tumourous stem mustard and its response to abiotic stresses
  19. Zoology
  20. First report of Gussevia asota (Monogenea: Dactylogyridae), destructive parasite of A stronotus ocellatus (Perciformes: Cichlidae) in Europe
  21. Zoology
  22. Response of the carpet shell clam (Ruditapes decussatus) and the Manila clam (Ruditapes philippinarum) to salinity stress
  23. Zoology
  24. Biodiversity of zooplankton (Rotifera and crustacea) in water soldier (Stratiotes aloides) habitats
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  26. Testing for longitudinal zonation of macroinvertebrate fauna along a small upland headwater stream in two seasons
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  28. Aphids (Hemiptera: Aphididae) of different plant communities in an urban environment
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