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Molecular characterization and alternative splicing of a MYB transcription factor gene in tumourous stem mustard and its response to abiotic stresses

  • Weina Wang , Quan Sun , Chaowei Cai , Can Yang , Xin Liu , Xiaohong He , Huaizhong Jiang , Jianchuan Mo and Yingfan Cai EMAIL logo
Published/Copyright: June 16, 2016
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Biologia
From the journal Biologia Volume 71 Issue 5

Abstract

Tumourous stem mustard, a variant of Brassica juncea (Cruciferae), is an economically important crop. In a preliminary study, the expression of a fragment of a MYB (v-myb avian myeloblastosis viral oncogene homolog) gene was screened out during stem swelling. The four complete cDNA sequences of novel MYB genes were cloned by rapid amplification of cDNA ends based on the MYB sequence fragment. These sequences, which represent distinct alternatively spliced transcripts of a BjMYB1 (Brassica juncea var. tumida Tsen et Lee MYB gene 1), possessed a similar alternative splicing pattern to several reported MYB genes of other species. RT-PCR analysis revealed different expression patterns for these four spliced transcripts in various tissues. However, stem expression of BjMYB1 gene was detected in the non-swelling growth phase, and different expression patterns were detected at other stages during stem swelling. The expression levels of the transcripts differed after treatment with various phytohormones (e.g., ABA) and NaCl. In addition, prokaryotic expression and Western blotting showed that BjMYB1-2 encodes a 5.3 kDa protein while BjMYB1-3 encodes a 21.6 kDa protein. The overexpression of BjMYB1-3 and BjMYB1-4 in Arabidopsis thaliana showed earlier germination, promoting growth and having tolerance to abiotic stress such as osmotic stress or high salt. The research of this article set the foundation for further study of the function of MYB genes in tumourous stem mustard and other Brassica plants.

Key words: Brassica juncea var. tumida; alternative splicing; gene expression; MYB transcription factor; prokaryotic expression

Acknowledgements

This work was supported by the Natural Science Foundation of China (NSFC grant No. 31571724, 31071461, 31470354), 2014 Henan outstanding talent project (154200510006) and Chongqing Natural Science, Foundation (cstc2012jjA80037, cstc2015jA80006, cstc2013jcyA10005) and Chongqing Municipal Commission of Education (KJ130502, KJ130520, KJ1400424, KJ1500420).

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Received: 2015-8-1
Accepted: 2016-4-20
Published Online: 2016-6-16
Published in Print: 2016-5-1

© 2016 Institute of Botany, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2016-0066
Keywords for this article
Brassica juncea var. tumida; alternative splicing; gene expression; MYB transcription factor; prokaryotic expression

Articles in the same Issue

  1. Cellular and Molecular Biology
  2. A broad host range food-grade cloning vector for lactic acid bacteria
  3. Cellular and Molecular Biology
  4. Antimicrobial and morphogenic effects of emodin produced by A spergillus awamori WAIR120
  5. Cellular and Molecular Biology
  6. Herbal augmentation enhances malachite green bio degradation efficacy of Saccharomyces cerevisiae
  7. Cellular and Molecular Biology
  8. Efflux pump inhibitory activity of flavonoids isolated from Alpinia calcarata against methicillin-resistant Staphylococcus aureus
  9. Cellular and Molecular Biology
  10. Status of metal pollution in rivers flowing through urban settlements at Pune and its effect on resident microflora
  11. Cellular and Molecular Biology
  12. Use of N, N′-diacetylchitobiose in decreasing toxic effects of indoor air pollution by preventing oxidative DNA damage
  13. Botany
  14. The relationship between macrophyte assemblages and environmental variables in drainage and irrigation canals in Slovakia
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  16. Effects of ZnCl2 on ROS generation, plasma membrane properties, and changes in protein expression in grapevine root explants
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  18. Molecular characterization and alternative splicing of a MYB transcription factor gene in tumourous stem mustard and its response to abiotic stresses
  19. Zoology
  20. First report of Gussevia asota (Monogenea: Dactylogyridae), destructive parasite of A stronotus ocellatus (Perciformes: Cichlidae) in Europe
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