Startseite Herbal augmentation enhances malachite green bio degradation efficacy of Saccharomyces cerevisiae
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Herbal augmentation enhances malachite green bio degradation efficacy of Saccharomyces cerevisiae

  • Shivtej P. Biradar , Niraj R. Rane , Tejas S. Patil , Rahul V. Khandare , Sanjay P. Govindwar und Pankaj K. Pawar EMAIL logo
Veröffentlicht/Copyright: 16. Juni 2016
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Biologia
Aus der Zeitschrift Biologia Band 71 Heft 5

Abstract

Saccharomyces cerevisiae was able to degrade a highly toxic textile dye malachite green (MG) at 100 mg/L concentration. Although 99% decolourization was observed, a tremendous metabolic and oxidative stress was exerted on the cells. Ethanolic extracts of Terminalia chebula, Clitoria ternatea and Boerhaavia diffusa at a concentration of 1 mg/mL were independently supplied to S. cerevisiae cells to counter the stress. T. chebula, C. ternatea and B. diffusa extracts reduced the activities of glutathione peroxidase (67, 8 and 71%), superoxide dismutase (2, 7 and 16%) and catalase (16, 52 and 57%), respectively. Inductions in the activities of laccase (66, 82 and 50%), lignin peroxidase (35, 75 and 10%), NADH-DCIP reductase (43, 52 and 91%) and MG reductase (66, 126 and 117%) were observed respectively. Presence of dye (MG) extended the lag phase of the growth cycle of S. cerevisiae up to 36 h, which was observed to be restored to normal (4 h) after phytoextract supplementation. Scanning electron microscope imaging revealed the restored cell morphology upon exposure to plant extracts. The accumulation of reactive oxygen species (ROS) was observed to be 355% greater in cells exposed to MG, which was significantly reduced after phytoextracts augmentation when compared to control cells. Phytoextracts proved to be beneficial in increasing the viability of S. cerevisiae cells and reduced the intracellular ROS and nuclear damage. Inclusion of plant extracts during decolourization proved to be beneficial and protected the cells so that 20 treatment cycles could be run achieving significant removal of MG.

Key words: malachite green; decolourization; Saccharomyces cerevisiae; oxidative stress; herbal extracts; toxicity

Acknowledgements

Authors are thankful to DBT, New Delhi, for funding in the form of DBT-SUK IPLS Program through grant No. BT/PR4572/INF/22/147/2012, SAP-DRS II Program, UGC New Delhi, for infrastructure facility and DST-PURSE program (Grant No.: SR/PURSE/2010) funded by DST, New Delhi, and funds for providing fellowship to one of the authors (SPB).

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Abbreviations
ABTS

2,2-azino-bis-3-ethyl benzothiazoline-6-sulphonic acid

a.u

arbitrary unit

CFUs

colony forming units

DAPI

4',6-diamidino-2-phenylindole

H2DCF

2'7'-dichlorofluorescin

MG

malachite green

ROS

reactive oxygen species

SEM

scanning electron microscope.

Received: 2015-12-15
Accepted: 2016-4-14
Published Online: 2016-6-16
Published in Print: 2016-5-1

© 2016 Institute of Molecular Biology, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2016-0069
Schlagwörter für diesen Artikel
malachite green; decolourization; Saccharomyces cerevisiae; oxidative stress; herbal extracts; toxicity

Artikel in diesem Heft

  1. Cellular and Molecular Biology
  2. A broad host range food-grade cloning vector for lactic acid bacteria
  3. Cellular and Molecular Biology
  4. Antimicrobial and morphogenic effects of emodin produced by A spergillus awamori WAIR120
  5. Cellular and Molecular Biology
  6. Herbal augmentation enhances malachite green bio degradation efficacy of Saccharomyces cerevisiae
  7. Cellular and Molecular Biology
  8. Efflux pump inhibitory activity of flavonoids isolated from Alpinia calcarata against methicillin-resistant Staphylococcus aureus
  9. Cellular and Molecular Biology
  10. Status of metal pollution in rivers flowing through urban settlements at Pune and its effect on resident microflora
  11. Cellular and Molecular Biology
  12. Use of N, N′-diacetylchitobiose in decreasing toxic effects of indoor air pollution by preventing oxidative DNA damage
  13. Botany
  14. The relationship between macrophyte assemblages and environmental variables in drainage and irrigation canals in Slovakia
  15. Botany
  16. Effects of ZnCl2 on ROS generation, plasma membrane properties, and changes in protein expression in grapevine root explants
  17. Botany
  18. Molecular characterization and alternative splicing of a MYB transcription factor gene in tumourous stem mustard and its response to abiotic stresses
  19. Zoology
  20. First report of Gussevia asota (Monogenea: Dactylogyridae), destructive parasite of A stronotus ocellatus (Perciformes: Cichlidae) in Europe
  21. Zoology
  22. Response of the carpet shell clam (Ruditapes decussatus) and the Manila clam (Ruditapes philippinarum) to salinity stress
  23. Zoology
  24. Biodiversity of zooplankton (Rotifera and crustacea) in water soldier (Stratiotes aloides) habitats
  25. Zoology
  26. Testing for longitudinal zonation of macroinvertebrate fauna along a small upland headwater stream in two seasons
  27. Zoology
  28. Aphids (Hemiptera: Aphididae) of different plant communities in an urban environment
  29. Zoology
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  31. Cellular and Molecular Biology
  32. Ependymal tables designated for differentiation of the ependyma based on the adjacent periventricular structures
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