Abstract
A 3-year field research was conducted to investigate the effect of four subsoiling methods on maize yield and water use efficiency. These four subsoiling methods included bulk subsoiling in autumn (BS), annual ridge subsoiling in autumn, annual furrow subsoiling in early summer, and interannual alternate zone subsoiling (AS), which were compared with rotary tillage in spring. The results suggested that the bulk density of the soil was alternately changed by AS, but AS had limited effect on the average water storage in the soil. Overall, soil tilled by BS and AS maintained a high water-holding capacity, which could stabilize the yield of maize under varying rainfall across different years. Maize under the AS subsoiling method maintained a relatively higher production capacity and water-use efficiency compared with those under the other three types of subsoiling methods.
1 Introduction
Water resources are often a limiting factor for the sustainable development of agricultural systems, especially in semiarid areas [1]. The ability of the soil layer to hold water in the root zone is important to increase crop yield and water-use efficiency (WUE) as it reduces drought risk during the crop-growing season. Moreover, subsoiling has a direct influence on soil moisture [2,3]. Rain-fed agriculture often faced problems of poor land management due to decentralized farm land, sandy soil, or low economic profit. For example, in the north region of Liaoning in northeast China, where the physical and chemical properties of the topsoil for growing crops are poor and the plow pan is thick and uplifted, the root penetration is limited, reducing the capacity of holding rainfall [4].
Subsoiling, as a commonly used agricultural practice, can effectively loosen soil for receiving and holding more rainfall in the soil and breaking the plow pan for the improvement in water percolation and root penetration [3,4]. The loose soil in the upper layer and compact soil in the lower layer of the root zone are favorable for maize growth and effectively prevent maize stalk lodging in the late season [5]. Subsoiling reduces surface runoff and increases the water availability of the soil, thus improving the potential of boosting crop growth, yield, WUE, and sustainability [6, 7, 8]. Partial subsoiling of alternate strips of tilled and untilled ground in a field was found to increase the water- and nutrient-holding capacity of the soil and reduce soil evaporation [9, 10, 11].
Interannual alternate zone subsoiling (AS) mainly involves the alternate application of ridge subsoiling after the autumn harvest and furrow subsoiling before the elongation of the maize stalk in early summer of the second year. Information regarding the effect of subsoiling methods on the water-holding capacity of the soil is limited in published scientific studies.
Therefore, the objective of this study was to (a) quantify the effects of the intensity and proportion of subsoiling on crop yield, soil physical properties, water uptake, and WUE in rain-fed agriculture; and (b) explore the mechanism of soil improvement and water availability by full and partial subsoiling. The results were used to discuss the application of optimal subsoiling practice in the semiarid region for the sustainable development of rain-fed agriculture in relation to soil erosion and climate change.
2 Materials and Methods
2.1 Experiment Description
This study was conducted at the Experiment Station of Agricultural Environment and Arable Land Conservation in Fuxin, Liaoning, China, located at longitude 121.46, latitude 42.09, and an elevation of 213 m. Zhengdan 958 maize seeds were sown in brown earth on the experimental fields after cleaning the stubble through shallow rotary tillage (to a depth of about 8 cm). The field was free of deep tillage for more than 10 years before the initiation of the experiment. In 2012, 2013, and 2014, the total precipitation in this region was 483.7, 462.3, and 216.3 mm, respectively. Irrigation was not conducted during the experimentation.
2.2 Experimental Design
Five treatments, including bulk subsoiling in autumn (BS; work date was around October 25 every year, and the depth was 28 cm), annual ridge subsoiling in autumn (RS; work date was the same as BS, and the depth was 28 cm), annual furrow subsoiling in early June before maize elongation (FS; the depth was 28 cm), and interannual AS (1 year RS and next year FS; the depth was 28 cm), were implemented and compared with rotary tillage in spring (CK; the depth was 15 cm). Figure 1a shows the subsoiler used for ridge subsoiling, furrow subsoiling, and interannual AS, and Figure 1b shows the subsoiler used for bulk subsoiling. The experimental design was a randomized design with three replicates. The plot size was 30 m in length and 10 m in width.

Two kinds of subsoilers.
The maize was planted on the ridge and rowed one line on each ridge; the ridge spacing was 50 cm. The plant density of maize was 60,000 plants/ha (with row distance of 50 cm and planting distance of 33 cm), and NPK compound fertilizer (the contents of N, P2O5, and K2O were 15%) was used at a rate of 375 kg/ha. A topdressing of urea with N content of 46% was applied before the maize elongation stage at a rate of 375 kg/ha. Maize was sowed on April 29 and 30, and May 6, and harvested on September 25 and 27, and October 21, in 2012, 2013, and 2014, respectively.
Ethical approval
The conducted research is not related to either human or animals use.
2.3 Measurements
2.3.1 Bulk density of the soil
The bulk densities of the soils were measured using a foil sampler immediately after the maize was harvested. Soils were sampled under the ridge and furrow in each plot; 5 cm was measured for each layer, averaging three iterations. The bulk densities of the soils at depths of 0–15 cm and 15–30 cm were calculated according to Equation 1 using the average of corresponding levels.
where ρ is the bulk density of the soil of one sample, g/cm3; m is the dry weight of the soil in the foil sampler, g; and ν is the sample volume of the foil sampler, cm3.
2.3.2 Moisture content of the soil
The water content in the soil was determined from the date of planting to harvesting and determined once a month. It was measured using an earth boring auger and then oven dried at 150°C. The sampling point in each sampling layer and at the junction of ridge and furrow was 10 cm, and the measuring depth was 100 cm. The water storage in the soil was calculated using Equation 2. The bulk density of the soil at a depth of 0–30 cm was calculated according to the measurements obtained that correlated to each year, and the water content in the soil at a depth of 30–100 cm was calculated according to the average bulk density of 1.46 g/cm3 of the soil. The bulk density of the soil at a depth of 30–100 cm changed a little as the depth of tillage was less than 30 cm.
where W is the water storage in the soil, mm; φi is the water content in the soil for each soil layer; ρi is the bulk density of the soil for each soil layer, g/cm3; hi is the sampling depth (one soil layer), mm.
2.3.3 Yield
Maize yield for each sample plot (50 m2) was measured once maize ripened.
2.4 Data Analysis
2.4.1 Water uptake
The soil water balance method was used to calculate the water uptake in this study, according to Equation 3.
where ET is the water consumption of the soil, mm; I and P represent irrigation and rainfall, respectively, of that period, mm; RO is the soil surface runoff for rainfall or irrigation, mm; DP is the deep soil percolation, mm; CR is the groundwater rising from the capillary to the root zone (due to lower groundwater levels, generally ignored), mm; ΔSF is the lateral leakage of the soil, including the lateral inflow SFin and lateral outflow SFout (ignored in this test), mm; and ΔSW is the soil moisture variation, mm.
2.4.2 Water-use efficiency
The WUE of the crops was calculated using Equation 4 [12].
where WUE is the water-use efficiency, kg/m3; Y is the yield of maize, kg/ha; and ET is the soil water evapotranspiration, mm.
3 Results
3.1 Effects of Different Tillage Methods on the Bulk Density of the Soil
After harvesting, vertical changes occurred in the bulk density of the soil, as shown in Table 1. The results showed that the fields tilled with subsoiling methods significantly reduced the bulk density of the soil compared with CK. The most significant reduction in bulk density was observed using BS and AS tillage methods in 2012 and 2014. In 2013, the most effective reduction in the bulk densities of the soils was noted using the BS method. In different tillage methods, the average bulk densities of 0–15 cm of the soil layer of CK were at the lowest level in the 3 years; the hierarchical orders were as follows: RS > FS > BS ≈ AS > CK in 2012; AS > FS ≈ RS > BS > CK in 2013; and FS ≈ RS > AS ≈ BS ≈ CK in 2014. Although the average bulk densities of 15–30 cm of the soil layer of CK were at the highest level, the order was as follows: CK > RS > FS > AS ≈ BS in 2012; CK > RS ≈ AS ≈ FS > BS in 2013; and CK > RS ≈ FS > AS ≈ BS in 2014.
Soil bulk density under different subsoiling treatments in 2011–2014 (unit: g/cm3)
2011 | 2012 | 2013 | 2014 | |||||
---|---|---|---|---|---|---|---|---|
0–15 cm | 15–30 cm | 0–15 cm | 15–30 cm | 0–15 cm | 15–30 cm | 0–15 cm | 15–30 cm | |
CK | 1.299 aA | 1.459 aA | 1.272 dD | 1.465 aA | 1.261 dC | 1.464 aA | 1.280 bB | 1.459 aA |
BS | 1.300 aA | 1.457 aA | 1.281 cC | 1.369 dD | 1.277 cB | 1.359 dD | 1.278 bB | 1.364 cB |
RS | 1.301 aA | 1.451 aA | 1.304 aA | 1.403 bB | 1.292 bA | 1.392 bB | 1.290 aA | 1.379 bB |
FS | 1.313 aA | 1.442 aA | 1.295 bB | 1.382 cC | 1.292 bA | 1.377 cC | 1.293 aA | 1.372 bB |
AS | 1.292 aA | 1.451 aA | 1.281 cC | 1.370 dD | 1.304 aA | 1.388 bBC | 1.279 bB | 1.365 cB |
P | 0.371 | 0.218 | 0.000 | 0.000 | 0.013 | 0.000 | 0.002 | 0.000 |
BS, bulk subsoiling in autumn; RS, annual ridge subsoiling in autumn; FS, annual furrow subsoiling in early summer; AS, interannual alternate zone subsoiling; CK, rotary tillage in spring.
Values followed by the same small letter are not significantly different according to least significant difference (LSD) at 0.05 level in the same year, Values followed by the capital letter are not significantly different according to LSD at 0.01 level in the same year.
The lateral variations in bulk density of the soil caused by different tillage methods were also measured (Table 2). The average bulk densities of 15–30 cm of the soil layer of CK were at the highest level regardless of ridge or furrow. The hierarchical order in the ridge position was CK > FS > RS ≈ AS ≈ BS in 2012; CK > FS ≈ AS > FS ≈ BS in 2013; and CK > FS > AS ≈ RS ≈ BS in 2014. The hierarchical order in the furrow position was CK > RS > BS ≈ FS ≈ AS in 2012; CK ≈ RS > AS > BS ≈ FS in 2013; and CK > RS > BS ≈ AS ≈ FS in 2014.
Lateral variations of soil bulk density under different treatments (unit: g/cm3)
In 2011 | In 2012 | In 2013 | In 2014 | |||||
---|---|---|---|---|---|---|---|---|
Ridge | Furrow | Ridge | Furrow | Ridge | Furrow | Ridge | Furrow | |
CK | 1.358 aA | 1.400 aA | 1.349 aA | 1.387 aA | 1.344 aA | 1.381 aA | 1.352 aA | 1.387 aA |
BS | 1.353 aA | 1.404 aA | 1.307 cC | 1.342 cC | 1.299 cC | 1.337 cC | 1.300 dC | 1.343 cC |
RS | 1.361 aA | 1.391 aA | 1.311 cC | 1.397 bB | 1.304 cC | 1.380 aA | 1.303 cdC | 1.366 bB |
FS | 1.348 aA | 1.407 aA | 1.339 bB | 1.339 cC | 1.338 bAB | 1.331 cC | 1.331 bB | 1.334 cC |
AS | 1.351 aA | 1.392 aA | 1.312 cC | 1.339 cC | 1.335 bB | 1.358 bB | 1.306 cC | 1.338 cC |
P | 0.415 | 0.170 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 |
The primary characteristics of the bulk densities of the soil as a result of using a variety of tilling methods are summarized as follows. The soil tilled by BS was uniformly loose. The soil cultivated by CK showed vertically uneven bulk densities, with relatively loose upper soil and compact lower soil. The soil tilled by the RS technique was characterized by loose ridge soil and compact furrow soil, while FS yielded the opposite result. The soil tilled by AS exhibited alternate change in bulk density and alternately loosened strips of ridge and furrow.
3.2 Effects of Different Tillage Methods on the Water Storage of the Soil
Figure 2a shows the effects of various tillage techniques on the water storage of the cultivated soil. In 2012, 2013, and the early growth stage of 2014, rainfall was sufficient, and the water-holding capacity of the soil greatly increased after subsoiling. In the late growth stage of 2014, an unusual mid-summer and autumn drought occurred in the region [13]. The drought sharply reduced the amount of water that was held in the soil. Perhaps the soil moisture of the subsoiling treatment was one of the key factors for the stable production of crops. The results showed that the average water storage of BS, AS, FS, and RS was 10.9, 10.9, 9.0, and 8.2 mm, respectively, more than CK in 2012 (Fig. 2b). During the maize growth period of 2013, the hierarchical ranking of the average water storage in the soil was BS > AS > FS > RS. The average water storage in the soil of BS, AS, FS, and RS was 16.3, 14.6, 12.7, and 6.0 mm more than CK, respectively. In 2014, the average water storage in the soil of BS and AS during the maize growth period was 23.2 and 17.2 mm more than CK, while that of FS and RS was 15.7 and 7.1 mm more than CK, respectively.

Change in soil water storage (0–100 cm). (a) Change in soil water storage in the study period; (b) average soil water storage in the maize growth period in different years. BS, bulk subsoiling in autumn; RS, annual ridge subsoiling in autumn; FS, annual furrow subsoiling in early summer; AS, interannual alternate zone subsoiling; CK, rotary tillage in spring.
3.3 Effects of Different Tillage Methods on the Yield and WUE of Crops
The yield and WUE of maize in different years were significantly different (Table 3). In 2012, the AS produced the highest yield followed by BS, RS, and FS, which was 18.93%, 17.22%, 11.01%, and 9.45% higher than CK, respectively (Table 3). Similarly, the result in 2014 showed that the AS produced the highest yield followed by BS, RS, and FS, which was 17.38%, 16.21%, 9.91%, and 5.90% higher than CK, respectively. In 2012 and 2013, rainfall was relatively abundant, and the use of subsoiling increased the water storage capacity of the soil and reduced the water consumption of farmland. During the crop growth period in 2012, the water consumption of the soil of AS was 22.3 mm less than CK. The water consumption of the soil of BS was 14.6 mm less than that of CK. In 2013, BS exhibited the least water consumption in the soil, which was 26.9 mm less than CK.
Maize yield and water-use efficiency under different subsoiling treatments in 2012–2014
Year | Treatment | Yield (kg/ha) | Water consumption (mm) | WUE (kg/m3) |
---|---|---|---|---|
2012 | CK | 9473.6 d | 459.4 a | 2.06 d |
BS | 11507.3 b | 444.8 d | 2.59 b | |
RS | 10738.4 c | 455.8 b | 2.36 c | |
FS | 10726.5 c | 451.8 c | 2.37 c | |
AS | 12188.0 a | 437.1 e | 2.79 a | |
2013 | CK | 10752.3 c | 433.2 a | 2.48 c |
BS | 12788.03 a | 406.3 c | 3.15 a | |
RS | 11936.2 b | 421.5 b | 2.83 b | |
FS | 11768.0 b | 413.1 b | 2.85 b | |
AS | 12603.9 a | 416.1 b | 3.03 a | |
2014 | CK | 8172.0 e | 303.2 b | 2.70 c |
BS | 9497.0 b | 326.0 a | 2.91 b | |
RS | 8981.5 c | 305.8 b | 2.94 ab | |
FS | 8654.0 d | 312.7 a | 2.77 b | |
AS | 9592.2 a | 311.7 b | 2.97 a |
A severe drought occurred during the 2014 experimentation period. A large amount of water stored in the soil was consumed during the whole growth period for BS and FS, which was 22.8 and 9.5 mm more than CK, respectively. The water consumption of AS, RS, and CK was not significant. The comparison of WUE of different treatments showed that it was the highest in 2012 by AS, followed by BS, FS, and RS, which was 0.73, 0.53, 0.31, and 0.30 kg/cm3 more than CK, respectively. WUE was the highest in 2013 by BS and AS, followed by FS and RS, which was 0.67, 0.55, 0.37, and 0.35 kg/cm3 more than CK, respectively. Moreover, the results in 2014 showed that WUE was 0.27, 0.24, 0.21, and 0.07 kg/cm3 more by AS, RS, BS, and FS, respectively, compared with CK.
4 Discussion and Conclusion
Cultivation is an important agricultural practice in China, as farmland is seldom left fallow. Intense tillage and land cultivation have been a part of Chinese agriculture for thousands of years [14]. Subsoiling can reach profound soil layers and break up the plow pan. Therefore, soil conditions are usually improved by reducing the bulk density of soil and increasing soil permeability. This practice can facilitate the filtration of rainwater and increase the water content on the topsoil, thus promoting crop production [15,16].
The results of this 3-year field research revealed the effect of different tillage methods on the bulk density of the soil. The soil tilled by the BS technique was uniformly loose, and the soil tilled by the CK technique showed vertically uneven bulk densities, with relatively loose upper soil and compact lower soil. The soil tilled by the RS technique was characterized by loose ridge soil and compact furrow soil, while the FS-tilled soil yielded the opposite result. The soil tilled by the AS technique showed alternating changes in bulk density and alternately loosened strips of ridge and furrow. Previous studies demonstrated that the bulk density of the soil was closely related to the water storage capacity of the soil [17,18]. Subsoiling operations were carried out in the farmland ridge and furrow in the autumn of 2011 and early summer of 2012, respectively. The soil was not tilled deeply during the period from the 2012 crop harvest to the 2013 crop harvest. Then, subsoiling operations were carried out in the autumn of 2013 and early summer of 2014, respectively. Consequently, the bulk densities of the soil varied across the different years. However, the findings of the present research indicated that the variations in bulk densities might not affect the water storage capacity of the soil. AS and BS maintained a high water storage in the soil, which could stabilize the maize yield across years with varied rainfalls. This could be attributed to the subsoiling with aftereffect [19,20], and soil moisture retention effect across years [2]. The increased range of the average water storage in the soil during the crop growth period by BS, AS, FS, and RS was 10.9–23.2, 10.9–17.2, 9.0–15.7, and 6.0–8.2 mm, respectively, compared with CK in 3 years.
The findings of this 3-year field research suggested that subsoiling has the potential to create favorable growth conditions for maize by increasing the water content and permeability of the soil. Subsoiling methods were highly effective in increasing maize yield and WUE. Compared with the other three subsoiling methods, the AS method continued to maintain higher production capacity and WUE. Moreover, AS and BS were associated with similar soil water storage capacity. Previous studies suggested that the composite topsoil structure composed of both loose and compact soil layers in interval subsoiling could facilitate the upward movement of water to topsoil in the dry season and help crops resist lodging [4,5]. AS had the advantages of interval subsoiling and provided greater water storage capacity than BS. Consequently, AS produced higher WUE and yield compared with BS in 2012 and 2014. Compared with RS and FS, the alternate subsoiling method was superior for reducing the bulk density of the soil, improving the structure of the soil, increasing water storage capacity of the soil, and maintaining higher crop productivity. Bulk tillage usually consumed more energy than zone subsoiling [21], and thus AS was a cost-effective method of subsoiling. Overall, the results of this study suggest that the AS method is a more beneficial subsoiling method compared with the BS, RS, FS, and CK methods.
Acknowledgments
The study was conducted with the support of the National High-Level Talent Special Support Program of China (outstanding young scholars), the Special Fund for Agro-scientific Research in the Public Interest of China (201503105, 201303125), the China Postdoctoral Science Foundation (178093), and the Cultivation Plan for Youth Agricultural Science and Technology Innovative Talents of Liaoning Province (2014017).
Conflict of interest: Authors state no conflict of interest
Reference
[1] Wuest S.B., Williams J.D., Gollany H.T., Tillage and perennial grass effects on ponded infiltration for seven semi-arid loess soils, Journal of Soil and Water Conservation, 2006, 61(2), 218-223Search in Google Scholar
[2] Lampurlanés J., Plaza-Bonilla D., Álvaro-Fuentes J., Cantero- Martínez C., Long-term analysis of soil water conservation and crop yield under different tillage systems in Mediterranean rainfed conditions, Field Crops Research, 2016, 189, 59-6710.1016/j.fcr.2016.02.010Search in Google Scholar
[3] Sang X., Wang D., Lin X., Effects of tillage practices on water consumption characteristics and grain yield of winter wheat under different soil moisture conditions, Soil & Tillage Research, 2016, 163, 185–19410.1016/j.still.2016.06.003Search in Google Scholar
[4] Bai W., Sun Z., Zheng J., Hao W., Liu Q., Liu Y., et al., Furrow loose and ridge compaction plough layer improves spring maize yield and water use efficiency, Transactions of the Chinese Society of Agricultural Engineering, 2014, 21(11), 81-90Search in Google Scholar
[5] Yang X., Pang H., Li Y., Ren T., Dong G., Guo Z., et al., Effects of deep rotary sub-soiling tillage on the physical properties and crop growth of the sticky loamy soil in north China, Scientia Agricultura Sinica, 2013, 46(16), 3401-3412Search in Google Scholar
[6] Ekelöf J., Guamán V., Jensen E. S., Persson P., Inter-Row Subsoiling and Irrigation Increase Starch Potato Yield, Phosphorus Use Efficiency and Quality Parameters, Potato Research, 2015, 58(1), 15-2710.1007/s11540-014-9261-5Search in Google Scholar
[7] McGarry D., Bridge B.J., Radford B.J., Contrasting soil physical properties after zero and traditional tillage of an alluvial soil in the semi- arid subtropics, Soil & Tillage Research, 2000, 53(2), 105-11510.1016/S0167-1987(99)00091-4Search in Google Scholar
[8] Ahmed S.M., Adeeb A., Topsoiling and Subsoiling as Rainwater Harvesting Techniques for Arid Climates: a Case Study from Sudan, Planet Risk, 2014, 2(1), 40-45Search in Google Scholar
[9] Abu-Hamdeh N.H., Compaction and sub-soiling effects on maize growth and soil bulk density, Soil Science Society of America Journal, 2003, 67(4), 1212-121810.2136/sssaj2003.1213Search in Google Scholar
[10] Martin-Rueda L., Munoz-Guerra L.M., Yunta F., Tillage and crop rotation effects on barley yield and soil nutrients on a Calciortidic Haploxeralf, Soil & Tillage Research, 2007, 92, 1-910.1016/j.still.2005.10.006Search in Google Scholar
[11] Tian S., Wang Y., Ning T., Li N., Zhao H., Wang B., et al., Continued no-till and subsoiling improved soil organic carbon and soil aggregation levels, Agronomy Journal, 2014, 106(1), 212-21810.2134/agronj2013.0288Search in Google Scholar
[12] Feng L.S., Sun Z.X., Yan C.R., Zheng M.Z., Zheng J.M., Yang N., et al., Effect of farmland surface covered porous mulch materials on soil water, heat and water use efficiency of maize, Journal of Animal and Plant Sciences, 2015, 25(S1), 22-27Search in Google Scholar
[13] Zhang L., Yan H., Cheng L., The summer of 2014, the influence of climate of the summer of 2014 on agricultural production, Chinese Journal of agrometeorology, 2014, 35(5), 600-602Search in Google Scholar
[14] Zuo S., Intensive cultivation is the soul of the sustainable development of agriculture in China, Agricultural Archaeology, 2003, (3), 50-55Search in Google Scholar
[15] Hemmat A., Eskandari I., Conservation tillage practices for winter wheat-fallow farming on a clay loam soil (Calcisols) under temperate continental climate of northwestern Iran, Field Crops Research, 2004, 89(1), 123-13310.1016/j.fcr.2004.01.019Search in Google Scholar
[16] Hemmat A., Eskandari I., Tillage system effects upon productivity of a dryland winter wheat-chickpea rotation in the northwest region of Iran, Soil & Tillage Research, 2004, 78(1), 69-8110.1016/j.still.2004.02.013Search in Google Scholar
[17] Gupta S.C., Larson W.E., Estimating soil water retention characteristics from particle size distribution, organic matter percent, and bulk density, Water Resources Research, 1979, 15(6), 1633-163510.1029/WR015i006p01633Search in Google Scholar
[18] Arya L.M., Paris J.F., A physicoempirical model to predict the soil moisture characteristic from particle-size distribution and bulk density data, Soil Science Society of America Journal, 1981, 45(6), 1023-103010.2136/sssaj1981.03615995004500060004xSearch in Google Scholar
[19] Dyck M., Malhi S.S., Nyborg M., Puurveen D., Effects of short-term tillage of a long-term no-till land on crop yield and nutrient uptake in two contrasting soil types, Sustainable Agriculture Research, 2016, 5(3), 3210.5539/sar.v5n3p32Search in Google Scholar
[20] Chaghazardi H.R., Jahansouz M.R., Ahmadi A., Gorji M., Effects of tillage management on productivity of wheat and chickpea under cold, rainfed conditions in western Iran, Soil & Tillage Research, 2016, 162, 26-3310.1016/j.still.2016.04.010Search in Google Scholar
[21] Guo X., Gu J., Analysis to the bulk subsoiling draft, Journal of Shanxi Teacher’s University Natural Science Edition, 1999, 13(4), 31-35Search in Google Scholar
© 2017 Yinzhu Jiao et al.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
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- Topical Issue On Precision Medicine
- The diagnostic value of serum microRNA-183 and TK1 as biomarkers for colorectal cancer diagnosis
- Topical Issue On Precision Medicine
- Screening feature modules and pathways in glioma using EgoNet
- Topical Issue On Precision Medicine
- Isoliquiritigenin inhibits colorectal cancer cells HCT-116 growth by suppressing the PI3K/AKT pathway
- Topical Issue On Precision Medicine
- Association between Caveolin-1 expression and pathophysiological progression of femoral nerves in diabetic foot amputation patients
- Topical Issue On Precision Medicine
- Biomarkers in patients with myocardial fibrosis
- Topical Issue On Precision Medicine
- Dysregulated pathways for off-pump coronary artery bypass grafting
- Topical Issue On Precision Medicine
- Individualized identification of disturbed pathways in sickle cell disease
- Topical Issue On Precision Medicine
- The prognostic value of serum PCT, hs-CRP, and IL-6 in patients with sepsis
- Topical Issue On Precision Medicine
- Sevoflurane-medicated the pathway of chemokine receptors bind chemokines in patients undergoing CABG
- Topical Issue On Precision Medicine
- The functional role of microRNAs in laryngeal carcinoma
- Topical Issue On Precision Medicine
- Revealing pathway cross-talk related to diabetes mellitus by Monte Carlo Cross-Validation analysis
- Topical Issue On Precision Medicine
- Correlation between CDKAL1 rs10946398C>A single nucleotide polymorphism and type 2 diabetes mellitus susceptibility: A meta-analysis
- Special Issue on Agricultural and Biological Sciences
- Effects of environmental variables on seedling distribution of rare and endangered Dacrydium pierrei
- Special Issue on Agricultural and Biological Sciences
- Study on synthesis and properties of nanoparticles loaded with amaryllidaceous alkaloids
- Special Issue on Agricultural and Biological Sciences
- Bacterial Infection Potato Tuber Soft Rot Disease Detection Based on Electronic Nose
- Special Issue on Agricultural and Biological Sciences
- Effects of subsoiling on maize yield and water-use efficiency in a semiarid area
Articles in the same Issue
- Research Articles
- Field Performance and Genetic Fidelity of Micropropagated Plants of Coffea canephora (Pierre ex A. Froehner)
- Research Articles
- Foliage maturity of Quercus ilex affects the larval development of a Croatian coastal population of Lymantria dispar (Lepidoptera: Erebidae)
- Research Articles
- Structural and functional impact of SNPs in P-selectin gene: A comprehensive in silico analysis
- Research Articles
- High embryogenic ability and regeneration from floral axis of Amorphophallus konjac (Araceae)
- Research Articles
- Terpene content of wine from the aromatic grape variety ‘Irsai Oliver’ (Vitis vinifera L.) depends on maceration time
- Research Articles
- Light and smell stimulus protocol reduced negative frontal EEG asymmetry and improved mood
- Research Articles
- Swailing affects seed germination of plants of European bio-and agricenosis in a different way
- Research Articles
- Survey analysis of soil physicochemical factors that influence the distribution of Cordyceps in the Xiahe Region of Gansu Province
- Research Articles
- Production of biogas: relationship between methanogenic and sulfate-reducing microorganisms
- Research Articles
- Pentraxin 3 and atherosclerosis among type 2 diabetic patients
- Research Articles
- Evaluation of ribosomal P0 peptide as a vaccine candidate against Argulus siamensis in Labeo rohita
- Research Articles
- Variation of autosomes and X chromosome STR in breast cancer and gynecological cancer tissues
- Research Articles
- Response of antioxidant enzymes to cadmium-induced cytotoxicity in rat cerebellar granule neurons
- Research Articles
- Cardiac hypertrophy and IGF-1 response to testosterone propionate treatment in trained male rats
- Research Articles
- BRAF-activated non-protein coding RNA (BANCR) advances the development of esophageal squamous cell carcinoma via cell cycle
- Research Articles
- The influence of soil salinity on volatile organic compounds emission and photosynthetic parameters of Solanum lycopersicum L. varieties
- Research Articles
- Simple Protocol for immunoglobulin G Purification from Camel “Camelus dromedarius” Serum
- Research Articles
- Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2
- Research Articles
- Frequency of Thrombophilic Gene Mutations in Patients with Deep Vein Thrombosis and in Women with Recurrent Pregnancy Loss
- Research Articles
- Evaluation of anticancer properties of a new α-methylene-δ-lactone DL-249 on two cancer cell lines
- Research Articles
- Impact of heated waters on water quality and macroinvertebrate community in the Narew River (Poland)
- Research Articles
- Effects of Some Additives on In Vitro True Digestibility of Wheat and Soybean Straw Pellets
- Research Articles
- RNAi-mediated gene silencing in Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae)
- Research Articles
- New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt
- Research Articles
- Tudor-SN protein expression in colorectal cancer and its association with clinical characteristics
- Research Articles
- Proteomic and bioinformatics analysis of human saliva for the dental-risk assessment
- Research Articles
- Reverse transcriptase sequences from mulberry LTR retrotransposons: characterization analysis
- Research Articles
- Strain Stimulations with Different Intensities on Fibroblast Viability and Protein Expression
- Research Articles
- miR-539 mediates osteoblast mineralization by regulating Distal-less genes 2 in MC3T3-E1 cell line
- Research Articles
- Diversity of Intestinal Microbiota in Coilia ectenes from Lake Taihu, China
- Research Articles
- The production of arabitol by a novel plant yeast isolate Candida parapsilosis 27RL-4
- Research Articles
- Effectiveness of Azospirillum brasilense Sp245 on young plants of Vitis vinifera L.
- Research Articles
- Changes of photochemical efficiency and epidermal polyphenols content of Prosopis glandulosa and Prosopis juliflora leaves exposed to cadmium and copper
- Research Articles
- Ultraweak photon emission in strawberry fruit during ripening and aging is related to energy level
- Research Articles
- Molecular cloning, characterization and evolutionary analysis of leptin gene in Chinese giant salamander, Andrias davidianus
- Research Articles
- Longevity and stress resistance are affected by activation of TOR/Myc in progenitor cells of Drosophila gut
- Research Articles
- Curcumin attenuates oxidative stress in liver in Type 1 diabetic rats
- Research Articles
- Risk factors of long-term postoperative renal function after partial nephrectomy in a solitary kidney
- Research Articles
- Developmental anomalies of the right hepatic lobe: systematic comparative analysis of radiological features
- Review articles
- Genetic Defects Underlie the Non-syndromic Autosomal Recessive Intellectual Disability (NS-ARID)
- Review articles
- Research Progress on Tissue Culture and Genetic Transformation of Kenaf (Hibiscus cannabinus)
- Topical Issue On Precision Medicine
- MiR-107 inhibits proliferation of lung cancer cells through regulating TP53 regulated inhibitor of apoptosis 1 (TRIAP1)
- Topical Issue On Precision Medicine
- The functional role of exosome microRNAs in lung cancer
- Topical Issue On Precision Medicine
- The diagnostic value of serum microRNA-183 and TK1 as biomarkers for colorectal cancer diagnosis
- Topical Issue On Precision Medicine
- Screening feature modules and pathways in glioma using EgoNet
- Topical Issue On Precision Medicine
- Isoliquiritigenin inhibits colorectal cancer cells HCT-116 growth by suppressing the PI3K/AKT pathway
- Topical Issue On Precision Medicine
- Association between Caveolin-1 expression and pathophysiological progression of femoral nerves in diabetic foot amputation patients
- Topical Issue On Precision Medicine
- Biomarkers in patients with myocardial fibrosis
- Topical Issue On Precision Medicine
- Dysregulated pathways for off-pump coronary artery bypass grafting
- Topical Issue On Precision Medicine
- Individualized identification of disturbed pathways in sickle cell disease
- Topical Issue On Precision Medicine
- The prognostic value of serum PCT, hs-CRP, and IL-6 in patients with sepsis
- Topical Issue On Precision Medicine
- Sevoflurane-medicated the pathway of chemokine receptors bind chemokines in patients undergoing CABG
- Topical Issue On Precision Medicine
- The functional role of microRNAs in laryngeal carcinoma
- Topical Issue On Precision Medicine
- Revealing pathway cross-talk related to diabetes mellitus by Monte Carlo Cross-Validation analysis
- Topical Issue On Precision Medicine
- Correlation between CDKAL1 rs10946398C>A single nucleotide polymorphism and type 2 diabetes mellitus susceptibility: A meta-analysis
- Special Issue on Agricultural and Biological Sciences
- Effects of environmental variables on seedling distribution of rare and endangered Dacrydium pierrei
- Special Issue on Agricultural and Biological Sciences
- Study on synthesis and properties of nanoparticles loaded with amaryllidaceous alkaloids
- Special Issue on Agricultural and Biological Sciences
- Bacterial Infection Potato Tuber Soft Rot Disease Detection Based on Electronic Nose
- Special Issue on Agricultural and Biological Sciences
- Effects of subsoiling on maize yield and water-use efficiency in a semiarid area