A genetic structure of novel population of Fascioloides magna from Poland, Podkarpackie Province, indicates an expanding second European natural focus of fascioloidosis

Ľudmila Juhásová; Eva Bazsalovicsová; Ivica Králová-Hromadová; Jacek Karamon

doi:10.1515/ap-2016-0109

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A genetic structure of novel population of Fascioloides magna from Poland, Podkarpackie Province, indicates an expanding second European natural focus of fascioloidosis

Ľudmila Juhásová , Eva Bazsalovicsová , Ivica Králová-Hromadová und Jacek Karamon

Veröffentlicht/Copyright: 24. Oktober 2016

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Aus der Zeitschrift Acta Parasitologica Band 61 Heft 4

Abstract

The veterinary important parasite of ruminants, giant liver fluke Fascioloides magna(Trematoda: Fasciolidae), isolated from liver of farmed fallow deer (Dama dama) from Podkarpackie Province (southeastern Poland) was genotypized by mitochondrial cytochrome c oxidase (cox1) and nicotinamide dehydrogenase (nad1) markers. The data on this newly emerged population were compared with mitochondrial haplotypes of recently detected Polish population of F. magna from Lower Silesian Wilderness (southwestern Poland) and with European populations of the parasite from all three natural foci; northern Italy, Czech Republic and the Danube floodplain forests. The flukes from Podkarpackie Province were found to be genetically identical with flukes from Czech Republic and Lower Silesian Wilderness in Poland. It is evident that central and southwestern Czech Republic, recognized as one of the endemic area of F. magna in Europe, has been enlarging and parasite has been invading several novel localities in Poland.

Keywords: Giant liver fluke; fallow deer; mitochondrial DNA; cox1; nad1; natural focus

Note

Nucleotide sequence data reported in this paper are available in the GenBankTM, EMBL and DDBJ databases under the accession numbers KX279954 - 5

Acknowledgements

The authors are thankful to Dr. Magdalena Larska (National Veterinary Research Institute in Pulawy, Poland) for providing the material. This work was supported by the Slovak Grand Agency VEGA (project VEGA no. 2/0133/13), Bilateral Mobility Project SAS-ASCR 16-20, and Centre of Excellence for Parasitology (ITMS26220120022; 0.4) supported by the Research & Development Operational Programme funded by the European Regional Development Fund.

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Received: 2016-5-31

Revised: 2016-6-24

Accepted: 2016-6-28

Published Online: 2016-10-24

Published in Print: 2016-12-1

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Artikel in diesem Heft

https://doi.org/10.1515/ap-2016-0109

Schlagwörter für diesen Artikel

Giant liver fluke; fallow deer; mitochondrial DNA; cox1; nad1; natural focus