Genotyping of Cryptosporidium spp. in environmental water in Turkey

Zeynep Koloren; Emine Ayaz

doi:10.1515/ap-2016-0094

Article

Genotyping of Cryptosporidium spp. in environmental water in Turkey

Zeynep Koloren and Emine Ayaz

Published/Copyright: October 22, 2016

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From the journal Acta Parasitologica Volume 61 Issue 4

Abstract

This research was undertaken to study the molecular detection and characterization of Cryptosporidium spp. in environmental water sources at Samsun and Giresun Provinces of The Black Sea in Turkey. Two-hundred forty and one-hundred eighty environmental samples were collected from a total of twenty and twenty-five sampling sites of Giresun and Samsun Provinces. One hundred twenty untreated drinking water samples were also detected for Cryptosporidium spp. in both investigated areas. 101 (%42), 92 (%38.3) of 240 and 74 (41.1%), 70 (38.8%) of 180 environmental samples have been found positive for Cryptosporidium spp. by Loop mediated isothermal amplification (LAMP) targeting the S-adenosyl-L-methionine synthetase (SAM) gene and nested PCR targeting small subunit (SSU)rRNA gene in Samsun and Giresun Provinces, respectively. Of the tested untreated drinking water samples collected from the investigated area, one sample was positive for Cryptosporidium spp. Six and twelve samples were clearly sequenced for the Cryptosporidium (SSU)rRNA gene among the highest positive samples selected from each of the twenty and twenty-five sampling sites of Giresun and Samsun Provinces, respectively. Genetic characterization of Cryptosporidium isolates from water samples represented Cryptosporidium bovis for five samples, Cryptosporidium parvum for six samples and one sample for Cryptosporidium felis in Samsun Province, where C. parvum for five samples and C. bovis for one sample were sequenced in Giresun Province. According to accessible information sources, this is the first research about genotyping of Cryptosporidium spp. in water samples collected from Samsun and Giresun Provinces of Turkey.

Keywords: Cryptosporidium spp; LAMP; (SSU)rRNA gene; Turkey

Acknowledgments

The study was supported by funds from The Scientific and Technological Research Council of Turkey – Project (TUBITAK-111T818) for two years. The authors are just responsible for this publication.

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Received: 2016-1-8

Revised: 2016-4-18

Accepted: 2016-5-23

Published Online: 2016-10-22

Published in Print: 2016-12-1

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Articles in the same Issue

https://doi.org/10.1515/ap-2016-0094

Keywords for this article

Cryptosporidium spp; LAMP; (SSU)rRNA gene; Turkey