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Effect of ampicillin-sulbactam on clinical capillary zone electrophoresis of serum proteins
-
Dominik Siede
Veröffentlicht/Copyright:
1. Oktober 2008
Abstract
Background: Capillary zone electrophoresis (CZE) is a well-accepted automated method used to separate serum proteins and detect monoclonal components. CZE uses ultraviolet detection at 214 nm to directly quantify proteins via peptide bonds. Any substance that absorbs at 214 nm and is present in serum can potentially interfere with CZE analysis. This has been reported for radio-contrast media and antibiotics.
Methods: Here we describe a peak on the anode side of the α2-globulin fraction caused by the antibiotic ampicillin-sulbactam (Unacid®).
Results and conclusions: Extra peaks that can be misinterpreted as monoclonal components can be present in almost all electrophoretic fractions of CZE. Immunosubtraction or immunofixation is always required to rule out these conditions.
Clin Chem Lab Med 2008;46:1468–9.
Received: 2008-3-24
Accepted: 2008-6-9
Published Online: 2008-10-01
Published in Print: 2008-10-01
©2008 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
capillary zone electrophoresis (CZE);
interference;
monoclonal component
Artikel in diesem Heft
- Review
- Use of biochemical markers of bone turnover in the management of postmenopausal osteoporosis
- Minireview
- Is serum procalcitonin able to predict long-term kidney morbidity from urinary tract infections in children?
- Genetics and Molecular Diagnostics
- Association between the 2756A> G variant in the gene encoding methionine synthase and myocardial infarction in Tunisian patients
- Common 894G>T single nucleotide polymorphism in the gene coding for endothelial nitric oxide synthase (eNOS) and risk of congenital heart defects
- New sequence variants in BRCA1 and BRCA2 genes detected by high-resolution melting analysis in an elderly healthy female population in Croatia
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- General Clinical Chemistry and Laboratory Medicine
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