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Measurement of 10,11-dihydro-10-hydroxy-carbamazepine in serum and plasma by high-performance liquid chromatography
-
Courtney Hunek
Veröffentlicht/Copyright:
29. August 2008
Abstract
Background: Oxcarbazepine is an antiepileptic drug (AED) used to treat partial seizures as a monotherapy or adjunctive therapy. Oxcarbazepine is metabolized in the liver to its active metabolite, 10,11-dihydro-10-hydroxy-carbamazepine (MHD). Optimal therapeutic effect and minimal toxicity can be achieved through monitoring MHD.
Methods: Serum/plasma was mixed with internal standard (5-ethyl-5-p-tolylbarbituric acid) in methanol and centrifuged for 10 min. The mobile phase consisted of phosphate buffer and acetonitrile (466:168 v/v). The supernatant was analyzed by liquid chromatography (LC) with UV detection at 210 nm.
Results: The linearity ranged from 1.0 to 50.0 μg/mL with analytical recovery of 101.3%–110.8%. The limit of quantification was 1.0 μg/mL. The intra-assay coefficient of variation (CV) was 7.0% and 2.0% for serum samples spiked at 16.3 and 35.8 μg/mL, respectively. The inter-assay CV was 4.3% and 2.3% for serum controls at 7.2 and 23.8 μg/mL, respectively. The method was correlated with a commercial LC-mass spectrometry method using 41 leftover patient samples containing MHD at concentrations of 1.0–36.4 μg/mL, showing a slope of 1.09, an intercept of 1.2 and r=0.9520. No interference was observed from commonly administered AEDs. Commercial controls containing more than 100 therapeutic drugs and endogenous substances were also tested and showed no interference.
Conclusions: This method is simple and effective for monitoring MHD in serum/plasma.
Clin Chem Lab Med 2008;46:1429–33.
Keywords: 10,11-dihydro-10-hydroxy-carbamazepine; high-performance liquid chromatography; oxcarbazepine
Received: 2008-5-7
Accepted: 2008-6-2
Published Online: 2008-08-29
Published in Print: 2008-10-01
©2008 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
10,11-dihydro-10-hydroxy-carbamazepine;
high-performance liquid chromatography;
oxcarbazepine
Artikel in diesem Heft
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- Use of biochemical markers of bone turnover in the management of postmenopausal osteoporosis
- Minireview
- Is serum procalcitonin able to predict long-term kidney morbidity from urinary tract infections in children?
- Genetics and Molecular Diagnostics
- Association between the 2756A> G variant in the gene encoding methionine synthase and myocardial infarction in Tunisian patients
- Common 894G>T single nucleotide polymorphism in the gene coding for endothelial nitric oxide synthase (eNOS) and risk of congenital heart defects
- New sequence variants in BRCA1 and BRCA2 genes detected by high-resolution melting analysis in an elderly healthy female population in Croatia
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