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Prolyl-hydroxyproline dipeptide in non-hydrolyzed morning urine and its value in postmenopausal osteoporosis
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Petr Hušek
Published/Copyright:
October 1, 2008
Abstract
Background: Owing to the high correlation between the level of prolyl-4-hydroxyproline dipeptide in non-hydrolyzed urine and that of 4-hydroxyproline in hydrolyzed urine, we examined whether the dipeptide might function as a valuable marker of bone turnover.
Methods: Based on densitometric measurements, 68 postmenopausal women were divided into groups of non-osteopathic, osteopenic and osteoporotic subjects. The dipeptide and current urinary resorption markers were assayed in morning urine, the former using liquid chromatography, the others plus serum formation markers by means of immunoassay procedures. Together with the assay of basal levels, diet-related changes and healing effect of yearly alendronate therapy were assessed.
Results: Concentration levels in controls and osteoporotic subjects differed significantly; receiver operating characteristics yielded sensitivity of 0.743, specificity of 0.908, area under curve of 0.903, and cut-off of 10.2 μmol/mmol of creatinine. Spearman rank correlation showed the highest pair coefficient between the dipeptide and osteocalcin. Diet-related changes were not found. Following therapy, a significant decline occurred already within a trimester, whilst with the other resorption markers not until 6 months.
Conclusions: The ease of the dipeptide assay in non-hydrolyzed urine surpasses that of hydroxyproline, and the results present the compound as a real competition to other commonly assessed markers in osteopathies.
Clin Chem Lab Med 2008;46:1391–7.
Keywords: alendronate therapy; diet-related effects; osteopathies and osteomarkers; postmenopausal osteoporosis; prolyl-hydroxyproline dipeptide
Received: 2008-2-6
Accepted: 2008-5-22
Published Online: 2008-10-01
Published in Print: 2008-10-01
©2008 by Walter de Gruyter Berlin New York
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Keywords for this article
alendronate therapy;
diet-related effects;
osteopathies and osteomarkers;
postmenopausal osteoporosis;
prolyl-hydroxyproline dipeptide
Articles in the same Issue
- Review
- Use of biochemical markers of bone turnover in the management of postmenopausal osteoporosis
- Minireview
- Is serum procalcitonin able to predict long-term kidney morbidity from urinary tract infections in children?
- Genetics and Molecular Diagnostics
- Association between the 2756A> G variant in the gene encoding methionine synthase and myocardial infarction in Tunisian patients
- Common 894G>T single nucleotide polymorphism in the gene coding for endothelial nitric oxide synthase (eNOS) and risk of congenital heart defects
- New sequence variants in BRCA1 and BRCA2 genes detected by high-resolution melting analysis in an elderly healthy female population in Croatia
- Enzyme-free signal amplification of analyte in a single closed tube by fluorescent hybridization chain reaction
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- Methionine metabolism in an animal model of sepsis
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