Startseite The in vivo and in vitro effects of L-carnitine supplementation on the erythrocyte membrane acetylcholinesterase, Na+, K+-ATPase and Mg2+-ATPase activities in basketball players
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The in vivo and in vitro effects of L-carnitine supplementation on the erythrocyte membrane acetylcholinesterase, Na+, K+-ATPase and Mg2+-ATPase activities in basketball players

  • Theodore Parthimos , Kleopatra H. Schulpis , Panagoula Angelogianni , Christi Tsopanakis , Nickolaos Parthimos und Stylianos Tsakiris
Veröffentlicht/Copyright: 1. Januar 2008
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Clinical Chemistry and Laboratory Medicine
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine Band 46 Heft 1

Abstract

Background: We investigated whether the activities of erythrocyte membrane acetylcholinesterase (AChE), Na+, K+-ATPase and Mg2+-ATPase are modulated in basketball players pre- vs. post-forced training with or without L-carnitine (L-C) supplementation.

Methods: Blood was obtained from 10 male players pre-game (group A) and post-game (group B) and after 1 month L-C supplementation (2 g/24 h orally) pre-training (group C) and post-training (group D). Lactate, pyruvate and total antioxidant status (TAS) were measured with commercial kits, catecholamines with HPLC and the enzyme activities spectrophotometrically.

Results: Lactate, pyruvate, AChE, Na+, K+-ATPase and catecholamines were increased (p<0.001) and TAS was decreased (p<0.001) in group B. In contrast, TAS remained unaltered and the all enzyme activities were reduced (p<0.001) in group D at the same time of study. Mg2+-ATPase activity remained unchanged. In vitro incubation of the modulated AChE and Na+, K+-ATPase with L-C (25 μM) from group B and group D resulted in a non-significant reduction of the enzymes in group B and complete restoration of their activities in group D.

Conclusions: The increase of AChE and Na+, K+-ATPase activities may be due to the elevation of catecholamines in group B. Carnitine utilization by the muscles during training may result in a reduction of the enzyme activities (group D). The latter is supported by the recovery of the enzyme activities after incubation of the membranes from group D with L-C.

Clin Chem Lab Med 2008;46:137–42.

Keywords: acetylcholinesterase; antioxidant status; carnitine; erythrocyte membranes; exercise; Na+, K+-ATPase
Corresponding author: Stylianos Tsakiris, PhD, Associate Professor, Department of Experimental Physiology, Medical School, University of Athens, Athens, Greece Phone: +30-210-7462662, Fax: +30-210-7462571,
Received: 2007-5-24
Accepted: 2007-8-30
Published Online: 2008-01-01
Published in Print: 2008-01-01

©2008 by Walter de Gruyter Berlin New York

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  28. Isoenzymes of N-acetyl-β-hexosaminidase in human pleomorphic adenoma and healthy salivary glands: a preliminary study
  29. The in vivo and in vitro effects of L-carnitine supplementation on the erythrocyte membrane acetylcholinesterase, Na+, K+-ATPase and Mg2+-ATPase activities in basketball players
  30. Validation and Outcome Studies
  31. Analytical evaluation of the Optium Xido blood glucose meter
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https://doi.org/10.1515/CCLM.2008.017
Schlagwörter für diesen Artikel
acetylcholinesterase; antioxidant status; carnitine; erythrocyte membranes; exercise; Na+, K+-ATPase

Artikel in diesem Heft

  1. Editorial
  2. Improving healthcare through advances in point-of-care technologies
  3. Review
  4. New creatinine sensor for point-of-care testing of creatinine meets the National Kidney Disease Education Program guidelines
  5. Original Papers
  6. Performance of a multi-profile critical care testing analyzer
  7. Comparative study of calculated and measured total carbon dioxide
  8. Letter to the Editor
  9. Performance of immunofluorometric point-of-care assays for free pregnancy-associated plasma protein A detection in whole blood samples
  10. Guidelines and Recommendations
  11. IFCC Guideline for sampling, measuring and reporting ionized magnesium in plasma
  12. Reviews
  13. Critical evaluation of 1H NMR metabonomics of serum as a methodology for disease risk assessment and diagnostics
  14. The entero-insular axis: implications for human metabolism
  15. Genetics and Molecular Diagnostics
  16. Functional polymorphisms in the promoter of the matrix metalloproteinase-9 (MMP-9) gene are not linked with significant plasma MMP-9 variations in healthy subjects
  17. Genetic profiling in healthy subjects from the Stanislas cohort based on 24 polymorphisms: effects on biological variables
  18. Relationship between methionine synthase, methionine synthase reductase genetic polymorphisms and deep vein thrombosis among South Indians
  19. Simultaneous genotyping of the three lactose tolerance-linked polymorphisms LCT –13907C>G, LCT –13910C>T and LCT –13915T>G with Pyrosequencing™ technology
  20. A new high-throughput screening method for the detection of chronic lymphatic leukemia and myelodysplastic syndrome
  21. General Clinical Chemistry and Laboratory Medicine
  22. Saliva analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF/MS): from sample collection to data analysis
  23. Mechanism of sodium loss with muscle sodium deficiency in sodium supplemented and unsupplemented subjects during hypokinesia
  24. Increased levels of 8-hydroxydeoxyguanosine and its relationship with lipid peroxidation and antioxidant vitamins in lung cancer
  25. Lipid peroxidation in stroke patients
  26. Resistin is present in human breast milk and it correlates with maternal hormonal status and serum level of C-reactive protein
  27. Waist-to-hip ratio correlates with homocysteine levels in male patients with coronary artery disease
  28. Isoenzymes of N-acetyl-β-hexosaminidase in human pleomorphic adenoma and healthy salivary glands: a preliminary study
  29. The in vivo and in vitro effects of L-carnitine supplementation on the erythrocyte membrane acetylcholinesterase, Na+, K+-ATPase and Mg2+-ATPase activities in basketball players
  30. Validation and Outcome Studies
  31. Analytical evaluation of the Optium Xido blood glucose meter
  32. Letters to the Editor
  33. New biomarkers of myocardial injury and assessment of cardiac toxicity during preparative regimen and hematopoietic cell transplantation in acute leukemia
  34. Analysis of serum and urinary lysophospholipase D/autotaxin in nephrotic syndrome
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