Secretory phospholipase A2 activity and release kinetics of vascular tissue remodelling biomarkers after coronary artery bypass grafting with and without cardiopulmonary bypass
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Shaida Varnous
Abstract
Background: Cardiopulmonary bypass (CPB) has long been recognised as a main cause of a postoperative complex systemic inflammatory response after coronary artery bypass grafting (CABG).
Methods: We determined the kinetics of peripheral blood release of the novel inflammatory biomarkers secretory phospholipase A2 (sPLA2), matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) during the first 6 days following surgery in 16 patients undergoing CABG with (on-pump, n=9) or without (off-pump, n=7) CPB. Kinetic curves for these markers were compared to those of the well-known inflammatory parameters C-reactive protein (CRP) and fibrinogen.
Results: sPLA2 activity exhibited a maximum value on day 2, then decreased until day 6 for both groups and in a similar manner as CRP levels. On the other hand, elevation of plasma levels of both MMP-9 and TIMP-1 occurred as early as on day 1 and remained at this level until day 6. No significant difference in kinetic characteristics (peak value, area under the curve, initial slope) between CABG with and without CPB was observed.
Conclusions: These data show that the off- and on-pump groups did not show significantly different kinetics for the releases of all biomarkers studied, including sPLA2 and biomarkers of the MMP-TIMP network. The off-pump procedure may therefore lead to global surgical trauma as important as CPB in terms of the systemic inflammatory process and matrix proteolysis pathway activation.
Clin Chem Lab Med 2007;45:372–5.
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©2007 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- From human genetic variations to prediction of risks and responses to drugs and the environment
- Nutrigenomics – 2006 update
- How to comprehensively analyse proteins and how this influences nutritional research
- Genotypes, obesity and type 2 diabetes – can genetic information motivate weight loss? A review
- The Gene-Diet Attica Investigation on childhood obesity (GENDAI): overview of the study design
- Polymorphisms in the APOA1/C3/A4/A5 gene cluster and cholesterol responsiveness to dietary change
- Nutri-epigenomics: lifelong remodelling of our epigenomes by nutritional and metabolic factors and beyond
- Emerging role of cathepsin S in obesity and its associated diseases
- Association analysis of hepatitis virus B infection with haplotypes of the TBX21 gene promoter region in the Chinese population
- Multiplex polymerase chain reaction on FTA cards vs. flow cytometry for B-lymphocyte clonality
- Real-time multiplex PCR assay for genotyping of three apolipoprotein E alleles and two choline acetyltransferase alleles with three hybridization probes
- Immunomagnetic CD45 depletion does not improve cytokeratin 20 RT-PCR in colorectal cancer
- Analysis of the components of hypertransaminasemia after liver resection
- Fine characterization of mitral valve glycosaminoglycans and their modification with degenerative disease
- Oxidative stress evaluated using an automated method for hydroperoxide estimation in patients with coronary artery disease
- Secretory phospholipase A2 activity and release kinetics of vascular tissue remodelling biomarkers after coronary artery bypass grafting with and without cardiopulmonary bypass
- Clustered components of the metabolic syndrome and platelet counts in Japanese females
- International Standard for serum vitamin B12 and serum folate: international collaborative study to evaluate a batch of lyophilised serum for B12 and folate content
- Multicentre physiological reference intervals for serum concentrations of immunoglobulins A, G and M, complement C3c and C4 measured with Tina-Quant® reagents systems
- In vivo and in vitro allergy diagnostics: it's time to reappraise the costs
- Experience with post-market surveillance of in-vitro diagnostic medical devices for lay use in Germany
- Evaluation of the high-sensitivity, full-range Olympus CRP OSR6199 application on the Olympus AU640®
- How to improve the teaching of urine microscopy
- In vitro determination of allergen-specific serum IgE. Comparative analysis of three methods
- Efficacy of a new blocker against anti-ruthenium antibody interference in the Elecsys free triiodothyronine assay
- Clinical indications for plasma protein assays: transthyretin (prealbumin) in inflammation and malnutrition: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division Committee on Plasma Proteins (C-PP)
- Meeting Report: From human genetic variations to prediction of risks and responses to drugs and the environment
