Evaluation of the high-sensitivity, full-range Olympus CRP OSR6199 application on the Olympus AU640®
-
Anne Marie Dupuy
Abstract
Background: Implementation of high-sensitivity C-reactive protein (hs-CRP) assays as a routine laboratory parameter may be necessary. A single CRP method giving reliable results for the whole concentration range (0.1–160 mg/L) is the most practical solution for a laboratory. The aim of this study was to assess the Olympus CRP full-range immunoturbidimetric assay on an Olympus AU640® biochemistry analyser.
Methods: CRP was determined in a population of patients simultaneously with the Randox CRP immunoturbidimetric assay and two immunonephelometric assays, the Dade Behring and Beckman Coulter CRP, on BN 100 and Immage systems, respectively.
Results: Analytical performance, including precision and linearity, was excellent. The correlation coefficient for linear regression was r2=0.998, indicating that the assay method exhibited good linearity in the working range 0–160 mg/L. The intra- and inter-assay CVs for the Olympus CRP method were <3.6% over a wide range of CRP concentrations. Linear regression analysis indicated excellent agreement between CRP values for all patients, with correlation coefficients of r2>0.996, 0.993 and 0.989 for the Randox, Dade Behring and Beckman methods, respectively. Bland-Altman analyses demonstrated the excellent concordance of the CRP methods for patient samples.
Conclusions: This study demonstrates that the new full-range Olympus method can be applied to measure CRP as a marker of inflammation and of cardiovascular risk.
Clin Chem Lab Med 2007;45:402–6.
References
1. Ridker PM, Buring JE, Shih J, Matias M, Hennekens CH. Prospective study of the C-reactive protein and the risk of future cardiovascular events among apparently healthy women. Circulation1998;98:731–3.10.1161/01.CIR.98.8.731Search in Google Scholar
2. Bassuk SS, Rifai N, Ridker PM. High-sensitivity C-reactive protein: clinical importance. Curr Probl Cardiol2004;29:439–93.10.1016/S0146-2806(04)00074-XSearch in Google Scholar
3. Pearson TA, Mensah GA, Alexander RW, Anderson JL, Cannon RO III, Criqui M, et al. Centers for Disease Control and Prevention; American Heart Association. Markers of inflammation and cardiovascular disease: application to clinical and public health practice: a statement for healthcare professionals from the Centers for Disease Control and Prevention and the American Heart Association. Circulation2003;107:499–511.10.1161/01.CIR.0000052939.59093.45Search in Google Scholar PubMed
4. Dupuy AM, Badiou S, Descomps B, Cristol JP. Immunoturbidimetric determination of C-reactive protein (CRP) and high-sensitivity CRP on heparin plasma. Comparison with serum determination. Clin Chem Lab Med2003;41:948–9.10.1515/CCLM.2003.144Search in Google Scholar PubMed
5. Roberts WL, Moulton L, Law TC, Farrow G, Cooper-Anderson M, Savory J, et al. Evaluation of nine automated high-sensitivity C-reactive protein methods: implications for clinical and epidemiological applications. Clin Chem2001;47:418–25.10.1093/clinchem/47.3.418Search in Google Scholar
6. Vassault A, Grafmeyer D, Naudin C, Dumont G, Bailly M, Henny J, et al., membres de la commission “Validation de techniques” de la SFBC. Protocole de validation de techniques. Ann Biol Clin1986;44:686–745.Search in Google Scholar
7. Bland JM, Altman DG. Measuring agreement in method comparison studies. Stat Methods Med Res1999;8:135–60.10.1191/096228099673819272Search in Google Scholar
8. Hollis S. Analysis of method comparison studies. Ann Clin Biochem1996;33:1–4.10.1177/000456329603300101Search in Google Scholar PubMed
©2007 by Walter de Gruyter Berlin New York
Articles in the same Issue
- From human genetic variations to prediction of risks and responses to drugs and the environment
- Nutrigenomics – 2006 update
- How to comprehensively analyse proteins and how this influences nutritional research
- Genotypes, obesity and type 2 diabetes – can genetic information motivate weight loss? A review
- The Gene-Diet Attica Investigation on childhood obesity (GENDAI): overview of the study design
- Polymorphisms in the APOA1/C3/A4/A5 gene cluster and cholesterol responsiveness to dietary change
- Nutri-epigenomics: lifelong remodelling of our epigenomes by nutritional and metabolic factors and beyond
- Emerging role of cathepsin S in obesity and its associated diseases
- Association analysis of hepatitis virus B infection with haplotypes of the TBX21 gene promoter region in the Chinese population
- Multiplex polymerase chain reaction on FTA cards vs. flow cytometry for B-lymphocyte clonality
- Real-time multiplex PCR assay for genotyping of three apolipoprotein E alleles and two choline acetyltransferase alleles with three hybridization probes
- Immunomagnetic CD45 depletion does not improve cytokeratin 20 RT-PCR in colorectal cancer
- Analysis of the components of hypertransaminasemia after liver resection
- Fine characterization of mitral valve glycosaminoglycans and their modification with degenerative disease
- Oxidative stress evaluated using an automated method for hydroperoxide estimation in patients with coronary artery disease
- Secretory phospholipase A2 activity and release kinetics of vascular tissue remodelling biomarkers after coronary artery bypass grafting with and without cardiopulmonary bypass
- Clustered components of the metabolic syndrome and platelet counts in Japanese females
- International Standard for serum vitamin B12 and serum folate: international collaborative study to evaluate a batch of lyophilised serum for B12 and folate content
- Multicentre physiological reference intervals for serum concentrations of immunoglobulins A, G and M, complement C3c and C4 measured with Tina-Quant® reagents systems
- In vivo and in vitro allergy diagnostics: it's time to reappraise the costs
- Experience with post-market surveillance of in-vitro diagnostic medical devices for lay use in Germany
- Evaluation of the high-sensitivity, full-range Olympus CRP OSR6199 application on the Olympus AU640®
- How to improve the teaching of urine microscopy
- In vitro determination of allergen-specific serum IgE. Comparative analysis of three methods
- Efficacy of a new blocker against anti-ruthenium antibody interference in the Elecsys free triiodothyronine assay
- Clinical indications for plasma protein assays: transthyretin (prealbumin) in inflammation and malnutrition: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division Committee on Plasma Proteins (C-PP)
- Meeting Report: From human genetic variations to prediction of risks and responses to drugs and the environment
Articles in the same Issue
- From human genetic variations to prediction of risks and responses to drugs and the environment
- Nutrigenomics – 2006 update
- How to comprehensively analyse proteins and how this influences nutritional research
- Genotypes, obesity and type 2 diabetes – can genetic information motivate weight loss? A review
- The Gene-Diet Attica Investigation on childhood obesity (GENDAI): overview of the study design
- Polymorphisms in the APOA1/C3/A4/A5 gene cluster and cholesterol responsiveness to dietary change
- Nutri-epigenomics: lifelong remodelling of our epigenomes by nutritional and metabolic factors and beyond
- Emerging role of cathepsin S in obesity and its associated diseases
- Association analysis of hepatitis virus B infection with haplotypes of the TBX21 gene promoter region in the Chinese population
- Multiplex polymerase chain reaction on FTA cards vs. flow cytometry for B-lymphocyte clonality
- Real-time multiplex PCR assay for genotyping of three apolipoprotein E alleles and two choline acetyltransferase alleles with three hybridization probes
- Immunomagnetic CD45 depletion does not improve cytokeratin 20 RT-PCR in colorectal cancer
- Analysis of the components of hypertransaminasemia after liver resection
- Fine characterization of mitral valve glycosaminoglycans and their modification with degenerative disease
- Oxidative stress evaluated using an automated method for hydroperoxide estimation in patients with coronary artery disease
- Secretory phospholipase A2 activity and release kinetics of vascular tissue remodelling biomarkers after coronary artery bypass grafting with and without cardiopulmonary bypass
- Clustered components of the metabolic syndrome and platelet counts in Japanese females
- International Standard for serum vitamin B12 and serum folate: international collaborative study to evaluate a batch of lyophilised serum for B12 and folate content
- Multicentre physiological reference intervals for serum concentrations of immunoglobulins A, G and M, complement C3c and C4 measured with Tina-Quant® reagents systems
- In vivo and in vitro allergy diagnostics: it's time to reappraise the costs
- Experience with post-market surveillance of in-vitro diagnostic medical devices for lay use in Germany
- Evaluation of the high-sensitivity, full-range Olympus CRP OSR6199 application on the Olympus AU640®
- How to improve the teaching of urine microscopy
- In vitro determination of allergen-specific serum IgE. Comparative analysis of three methods
- Efficacy of a new blocker against anti-ruthenium antibody interference in the Elecsys free triiodothyronine assay
- Clinical indications for plasma protein assays: transthyretin (prealbumin) in inflammation and malnutrition: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division Committee on Plasma Proteins (C-PP)
- Meeting Report: From human genetic variations to prediction of risks and responses to drugs and the environment
