Polymorphisms in the APOA1/C3/A4/A5 gene cluster and cholesterol responsiveness to dietary change
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Jaroslav A. Hubacek
Abstract
Background: The relationship between dietary composition and plasma lipids is to some extent genetically determined. It has been found that variants of some genes (e.g., apolipoprotein E and cholesterol 7-α hydroxylase) play an important role in changes in plasma lipid levels in response to dietary intervention. We analyzed the effect of variation in the apolipoprotein (APO) APOA1/C3/A4/A5 gene cluster on decreases in plasma cholesterol levels over an 8-year follow-up study.
Methods: Men (n=133) from the Czech population, for which dietary composition has markedly changed (red meat 80→68 kg/person/year, animal fat 16→9 kg/person/year, fruits and vegetables 133→150 kg/person/year) were recruited. APOA1 (G–75>A and C83>T), APOC3 (C–482>T and C3238>G), APOA4 (Thr347>Ser and Gln360His) and APOA5 (T–1131>C, Ser19>Trp and Val153>Met) variants were analyzed by PCR and restriction analysis. Lipid levels were analyzed in 1988 and 1996. Dietary information was obtained from the Institute of Agricultural Economy.
Results: In APOA5 Ser19Ser homozygotes (n=105), plasma cholesterol was relatively stable over the years (6.1±1.3 and 5.6±1.0 mmol/L in 1988 and 1996), but the decrease was much higher in Trp19 carriers (n=27; 6.5±1.6 vs. 5.1±1.1 mmol/L). This difference in change is significant at p<0.005. Similarly, a better response to dietary changes was detected in carriers of the common APOA4 haplotypes Thr-347Thr/Gln360Gln and Thr347Ser/Gln360Gln (n=102; 6.3±1.3 and 5.5±1.1 mmol/L in 1988 and 1996, p<0.001). Total cholesterol was relatively stable over time in carriers (n=18) of at least one His360 allele and/or two Ser347 alleles (5.7±1.1 and 5.5±0.9 mmol/L in 1988 and 1996, n.s.). Other variants analyzed did not influence the change in lipid measurements over time.
Conclusions: APOA4 and APOA5 variants may play an important role in the individual sensitivity of lipid parameters to dietary composition in men.
Clin Chem Lab Med 2007;45:316–20.
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©2007 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- From human genetic variations to prediction of risks and responses to drugs and the environment
- Nutrigenomics – 2006 update
- How to comprehensively analyse proteins and how this influences nutritional research
- Genotypes, obesity and type 2 diabetes – can genetic information motivate weight loss? A review
- The Gene-Diet Attica Investigation on childhood obesity (GENDAI): overview of the study design
- Polymorphisms in the APOA1/C3/A4/A5 gene cluster and cholesterol responsiveness to dietary change
- Nutri-epigenomics: lifelong remodelling of our epigenomes by nutritional and metabolic factors and beyond
- Emerging role of cathepsin S in obesity and its associated diseases
- Association analysis of hepatitis virus B infection with haplotypes of the TBX21 gene promoter region in the Chinese population
- Multiplex polymerase chain reaction on FTA cards vs. flow cytometry for B-lymphocyte clonality
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- Secretory phospholipase A2 activity and release kinetics of vascular tissue remodelling biomarkers after coronary artery bypass grafting with and without cardiopulmonary bypass
- Clustered components of the metabolic syndrome and platelet counts in Japanese females
- International Standard for serum vitamin B12 and serum folate: international collaborative study to evaluate a batch of lyophilised serum for B12 and folate content
- Multicentre physiological reference intervals for serum concentrations of immunoglobulins A, G and M, complement C3c and C4 measured with Tina-Quant® reagents systems
- In vivo and in vitro allergy diagnostics: it's time to reappraise the costs
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- Evaluation of the high-sensitivity, full-range Olympus CRP OSR6199 application on the Olympus AU640®
- How to improve the teaching of urine microscopy
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- Meeting Report: From human genetic variations to prediction of risks and responses to drugs and the environment
