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14-3-3 proteins in membrane protein transport

  • Thomas Mrowiec und Blanche Schwappach
Veröffentlicht/Copyright: 14. September 2006
Biological Chemistry
Aus der Zeitschrift Band 387 Heft 9

Abstract

14-3-3 proteins affect the cell surface expression of several unrelated cargo membrane proteins, e.g., MHC II invariant chain, the two-pore potassium channels KCNK3 and KCNK9, and a number of different reporter proteins exposing Arg-based endoplasmic reticulum localization signals in mammalian and yeast cells. These multimeric membrane proteins have a common feature in that they all expose coatomer protein complex I (COPI)- and 14-3-3-binding motifs. 14-3-3 binding depends on phosphorylation of the membrane protein in some and on multimerization of the membrane protein in other cases. Evidence from mutant proteins that are unable to interact with either COPI or 14-3-3 and from yeast cells with an altered 14-3-3 content suggests that 14-3-3 proteins affect forward transport in the secretory pathway. Mechanistically, this could be explained by clamping, masking, or scaffolding. In the clamping mechanism, 14-3-3 binding alters the conformation of the signal-exposing tail of the membrane protein, whereas masking or scaffolding would abolish or allow the interaction of the membrane protein with other proteins or complexes. Interaction partners identified as putative 14-3-3 binding partners in affinity purification approaches constitute a pool of candidate proteins for downstream effectors, such as coat components, coat recruitment GTPases, Rab GTPases, GTPase-activating proteins (GAPs), guanine-nucleotide exchange factors (GEFs) and motor proteins.

Keywords: 14-3-3 proteins; Arg-based ER localization signal; cell surface trafficking; coatomer protein complex I (COPI); multimeric membrane proteins; peptide sorting motifs; quality control
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Corresponding author

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Published Online: 2006-09-14
Published in Print: 2006-09-01

©2006 by Walter de Gruyter Berlin New York

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  11. 14-3-3 proteins in membrane protein transport
  12. The K+ channel gene, Kcnb1: genomic structure and characterization of its 5′-regulatory region as part of an overlapping gene group
  13. Structure-based specificity mapping of secreted aspartic proteases of Candida parapsilosis, Candida albicans, and Candida tropicalis using peptidomimetic inhibitors and homology modeling
  14. The solution structure of the membrane-proximal cytokine receptor domain of the human interleukin-6 receptor
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  17. Induction of intracellular signalling in human endothelial cells by the hyaluronan-binding protease involves two distinct pathways
  18. A novel proteolytically processed CDP/Cux isoform of 90 kDa is generated by cathepsin L
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https://doi.org/10.1515/BC.2006.152
Schlagwörter für diesen Artikel
14-3-3 proteins; Arg-based ER localization signal; cell surface trafficking; coatomer protein complex I (COPI); multimeric membrane proteins; peptide sorting motifs; quality control

Artikel in diesem Heft

  1. The arylhydrocarbon receptor: more than a tox story
  2. The aryl hydrocarbon receptor and light
  3. The impact of aryl hydrocarbon receptor signaling on matrix metabolism: implications for development and disease
  4. A role for the aryl hydrocarbon receptor in mammary gland tumorigenesis
  5. Evidence supporting the hypothesis that one of the main functions of the aryl hydrocarbon receptor is mediation of cell stress responses
  6. The arylhydrocarbon receptor repressor (AhRR): structure, expression, and function
  7. Impact of the arylhydrocarbon receptor on eugenol- and isoeugenol-induced cell cycle arrest in human immortalized keratinocytes (HaCaT)
  8. Aryl hydrocarbon receptor agonists directly activate estrogen receptor α in MCF-7 breast cancer cells
  9. Identifying target genes of the aryl hydrocarbon receptor nuclear translocator (Arnt) using DNA microarray analysis
  10. Transcriptional signatures of immune cells in aryl hydrocarbon receptor (AHR)-proficient and AHR-deficient mice
  11. 14-3-3 proteins in membrane protein transport
  12. The K+ channel gene, Kcnb1: genomic structure and characterization of its 5′-regulatory region as part of an overlapping gene group
  13. Structure-based specificity mapping of secreted aspartic proteases of Candida parapsilosis, Candida albicans, and Candida tropicalis using peptidomimetic inhibitors and homology modeling
  14. The solution structure of the membrane-proximal cytokine receptor domain of the human interleukin-6 receptor
  15. Sequence determination of lychnin, a type 1 ribosome-inactivating protein from Lychnis chalcedonica seeds
  16. Paired helical filaments contain small amounts of cholesterol, phosphatidylcholine and sphingolipids
  17. Induction of intracellular signalling in human endothelial cells by the hyaluronan-binding protease involves two distinct pathways
  18. A novel proteolytically processed CDP/Cux isoform of 90 kDa is generated by cathepsin L
  19. Degradation of apolipoprotein B-100 by lysosomal cysteine cathepsins
  20. Identification of trypsin I as a candidate for influenza A virus and Sendai virus envelope glycoprotein processing protease in rat brain
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