Identifying target genes of the aryl hydrocarbon receptor nuclear translocator (Arnt) using DNA microarray analysis
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Feng Wang
Abstract
The aryl hydrocarbon receptor nuclear translocator (Arnt) is a basic helix-loop-helix (bHLH) protein that also contains a Per-Arnt-Sim (PAS) domain. In addition to forming heterodimers with many other bHLH-PAS proteins, including the aryl hydrocarbon receptor (AhR) and hypoxia-inducible factors 1α, 2α and 3α, Arnt can also form homodimers when expressed from its cDNA in vitro or in vivo. However, target genes of the Arnt/Arnt homodimer remain to be identified. In this study, we have elucidated the profile of genes responsive to the reintroduction of Arnt expression in an Arnt-deficient mouse hepatoma cell line (c4), using DNA microarray analysis. The expression of 27 genes was upregulated by 1.5-fold or more in c4 cells infected with a retroviral vector expressing mouse Arnt, while no genes were found to be downregulated. Among the upregulated genes, BCL2/adenovirus E1B 19 kDa-interacting protein 1 (NIP3), serine (or cysteine) proteinase inhibitor, clade E, member 1 (PAI1), and N-myc downstream regulated-like (NDR1), were confirmed to be induced by Arnt using real-time PCR. We also found that the 5′ promoter region of 15 out of 20 upregulated genes contain the type 2 E-box 5′-CACGTG-3′ Arnt/Arnt binding sequence, consistent with the notion that they represent target genes for Arnt.
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Articles in the same Issue
- The arylhydrocarbon receptor: more than a tox story
- The aryl hydrocarbon receptor and light
- The impact of aryl hydrocarbon receptor signaling on matrix metabolism: implications for development and disease
- A role for the aryl hydrocarbon receptor in mammary gland tumorigenesis
- Evidence supporting the hypothesis that one of the main functions of the aryl hydrocarbon receptor is mediation of cell stress responses
- The arylhydrocarbon receptor repressor (AhRR): structure, expression, and function
- Impact of the arylhydrocarbon receptor on eugenol- and isoeugenol-induced cell cycle arrest in human immortalized keratinocytes (HaCaT)
- Aryl hydrocarbon receptor agonists directly activate estrogen receptor α in MCF-7 breast cancer cells
- Identifying target genes of the aryl hydrocarbon receptor nuclear translocator (Arnt) using DNA microarray analysis
- Transcriptional signatures of immune cells in aryl hydrocarbon receptor (AHR)-proficient and AHR-deficient mice
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- The solution structure of the membrane-proximal cytokine receptor domain of the human interleukin-6 receptor
- Sequence determination of lychnin, a type 1 ribosome-inactivating protein from Lychnis chalcedonica seeds
- Paired helical filaments contain small amounts of cholesterol, phosphatidylcholine and sphingolipids
- Induction of intracellular signalling in human endothelial cells by the hyaluronan-binding protease involves two distinct pathways
- A novel proteolytically processed CDP/Cux isoform of 90 kDa is generated by cathepsin L
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Articles in the same Issue
- The arylhydrocarbon receptor: more than a tox story
- The aryl hydrocarbon receptor and light
- The impact of aryl hydrocarbon receptor signaling on matrix metabolism: implications for development and disease
- A role for the aryl hydrocarbon receptor in mammary gland tumorigenesis
- Evidence supporting the hypothesis that one of the main functions of the aryl hydrocarbon receptor is mediation of cell stress responses
- The arylhydrocarbon receptor repressor (AhRR): structure, expression, and function
- Impact of the arylhydrocarbon receptor on eugenol- and isoeugenol-induced cell cycle arrest in human immortalized keratinocytes (HaCaT)
- Aryl hydrocarbon receptor agonists directly activate estrogen receptor α in MCF-7 breast cancer cells
- Identifying target genes of the aryl hydrocarbon receptor nuclear translocator (Arnt) using DNA microarray analysis
- Transcriptional signatures of immune cells in aryl hydrocarbon receptor (AHR)-proficient and AHR-deficient mice
- 14-3-3 proteins in membrane protein transport
- The K+ channel gene, Kcnb1: genomic structure and characterization of its 5′-regulatory region as part of an overlapping gene group
- Structure-based specificity mapping of secreted aspartic proteases of Candida parapsilosis, Candida albicans, and Candida tropicalis using peptidomimetic inhibitors and homology modeling
- The solution structure of the membrane-proximal cytokine receptor domain of the human interleukin-6 receptor
- Sequence determination of lychnin, a type 1 ribosome-inactivating protein from Lychnis chalcedonica seeds
- Paired helical filaments contain small amounts of cholesterol, phosphatidylcholine and sphingolipids
- Induction of intracellular signalling in human endothelial cells by the hyaluronan-binding protease involves two distinct pathways
- A novel proteolytically processed CDP/Cux isoform of 90 kDa is generated by cathepsin L
- Degradation of apolipoprotein B-100 by lysosomal cysteine cathepsins
- Identification of trypsin I as a candidate for influenza A virus and Sendai virus envelope glycoprotein processing protease in rat brain
