The Cytosine N4-Methyltransferase M.PvuII Also Modifies Adenine Residues
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Albert Jeltsch
Abstract
Methylation of DNA occurs at the C[5] and N[4] positions of cytosine and N[6] of adenine. The chemistry of methylation is similar among methyltransferases specific for cytosineN[4] and adenineN[6]. Moreover these enzymes have similar structures and active sites. Previously it has been demonstrated that the DNA(adenineN[6])methyltransferases M.EcoRV, M.EcoRI, E. coli dam and both domains of M.FokI also modify cytosine residues at the N[4] position [Jeltsch et al., J. Biol. Chem. 274 (1999), 19538 19544]. Here we show that the cytosineN[4] methyltransferase M.PvuII, which modifies the second cytosine in CAGCTG sequences, also methylates adenine residues in CAGATG/ CAGCTG substrates in which the target cytosine is replaced by adenine in one strand of the recognition sequence. Therefore, adenineN[6] and cytosine N[4] methyltransferases have overlapping target base specificities. These results demonstrate that the target base recognition by Nspecific DNA methyltransferases is relaxed in many cases. Furthermore, it shows that the catalytic mechanisms of adenine N[6] and cytosineN[4] methyltransferases are very similar.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Vaccine Development: from Empirical Medicine to Molecularly Designed Therapy
- Dendritic Cells for Specific Cancer Immunotherapy
- Intracellular Bacteria as Targets and Carriers for Vaccination
- Bacteria-Mediated Transfer of Eukaryotic Expression Plasmids into Mammalian Host Cells
- Revealing the Potential of DNA-Based Vaccination: Lessons Learned from the Hepatitis B Virus Surface Antigen
- Progress toward a Malaria Vaccine: Efficient Induction of Protective Anti-Malaria Immunity
- Peptide Vaccines and Peptide Libraries
- Defined Synthetic Vaccines
- Antimicrobial Peptides: Properties and Applicability
- G-Quadruplex DNA Structures Variations on a Theme
- The Role of Heat Shock Proteins and Their Receptors in the Activation of the Immune System
- Transcriptional Repression Mediated by the KRAB Domain of the Human C2H2 Zinc Finger Protein Kox1/ZNF10 Does Not Require Histone Deacetylation
- Structure and Evolution of 4-Coumarate:Coenzyme A Ligase (4CL) Gene Families
- Inhibition of Hepatitis B Virus by Hammerhead Ribozyme Targeted to the Poly(A) Signal Sequence in Cultured Cells
- Chemical Accessibility of 18S rRNA in Native Ribosomal Complexes: Interaction Sites of mRNA, tRNA and Translation Factors
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- The Cytosine N4-Methyltransferase M.PvuII Also Modifies Adenine Residues
- Expression of the Human Menkes ATPase in Xenopus laevis Oocytes
