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C-Terminal Peptides of Interleukin-6 Modulate the Expression of junB Protooncogene and the Production of Fibrinogen by HepG2 Cells
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Péter Igaz
Published/Copyright:
June 1, 2005
Abstract
Interleukin-6 (IL-6) is a 185 amino acid residue helical cytokine with various biological activities (e. g. B cell development, acute phase reaction). We have investigated the role of the 168 185 Cterminal region of IL-6 in the induction of fibrinogen synthesis and expression of junB mRNA using synthetic peptides corresponding to this region. Circular dichroism spectroscopy data suggest that even truncated peptides have a strong tendency to adopt an ordered conformation. Peptides were tested alone or in combination with recombinant hIL-6 on an IL-6 responsive human hepatoma HepG2 cell line. The expression of the protooncogene junB monitored by competitive RTPCR represents an early, while the fibrinogen production detected by sandwich ELISA a late, marker of IL-6 initiated events. We found that peptides depending on their structure modulate spontaneous as well as IL-6 induced fibrinogen production and/or mRNA expression of junB by exhibiting inhibition (in the presence of IL-6) or stimulation (in the absence of IL-6).
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Published Online: 2005-06-01
Published in Print: 2001-04-27
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Vaccine Development: from Empirical Medicine to Molecularly Designed Therapy
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- Intracellular Bacteria as Targets and Carriers for Vaccination
- Bacteria-Mediated Transfer of Eukaryotic Expression Plasmids into Mammalian Host Cells
- Revealing the Potential of DNA-Based Vaccination: Lessons Learned from the Hepatitis B Virus Surface Antigen
- Progress toward a Malaria Vaccine: Efficient Induction of Protective Anti-Malaria Immunity
- Peptide Vaccines and Peptide Libraries
- Defined Synthetic Vaccines
- Antimicrobial Peptides: Properties and Applicability
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- The Role of Heat Shock Proteins and Their Receptors in the Activation of the Immune System
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