Home Life Sciences Proteome Analysis by Three-Dimensional Protein Separation: Turnover of Cytosolic Proteins in Hepatocytes
Article
Licensed
Unlicensed Requires Authentication

Proteome Analysis by Three-Dimensional Protein Separation: Turnover of Cytosolic Proteins in Hepatocytes

  • Stefan Stevanovic and Peter Bohley
Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Volume 382 Issue 4

Abstract

We performed a threedimensional separation of pulsechase duallabelled rat liver cytosolic proteins using hydrophobic interaction chromatography, isoelectric focusing, and SDS gel electrophoresis. Due to very different expression rates but similar size and pI of rat liver cytosolic proteins, we demonstrate the impossibility of successful twodimensional separations of such complex protein mixtures. A prefractionation of proteins by hydrophobic interaction chromatography is therefore recommended prior to twodimensional gel electrophoresis. Our studies confirmed the correlation between protein turnover rates and surface hydrophobicity.

:
Published Online: 2005-06-01
Published in Print: 2001-04-27

Copyright © 2001 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Vaccine Development: from Empirical Medicine to Molecularly Designed Therapy
  2. Dendritic Cells for Specific Cancer Immunotherapy
  3. Intracellular Bacteria as Targets and Carriers for Vaccination
  4. Bacteria-Mediated Transfer of Eukaryotic Expression Plasmids into Mammalian Host Cells
  5. Revealing the Potential of DNA-Based Vaccination: Lessons Learned from the Hepatitis B Virus Surface Antigen
  6. Progress toward a Malaria Vaccine: Efficient Induction of Protective Anti-Malaria Immunity
  7. Peptide Vaccines and Peptide Libraries
  8. Defined Synthetic Vaccines
  9. Antimicrobial Peptides: Properties and Applicability
  10. G-Quadruplex DNA Structures Variations on a Theme
  11. The Role of Heat Shock Proteins and Their Receptors in the Activation of the Immune System
  12. Transcriptional Repression Mediated by the KRAB Domain of the Human C2H2 Zinc Finger Protein Kox1/ZNF10 Does Not Require Histone Deacetylation
  13. Structure and Evolution of 4-Coumarate:Coenzyme A Ligase (4CL) Gene Families
  14. Inhibition of Hepatitis B Virus by Hammerhead Ribozyme Targeted to the Poly(A) Signal Sequence in Cultured Cells
  15. Chemical Accessibility of 18S rRNA in Native Ribosomal Complexes: Interaction Sites of mRNA, tRNA and Translation Factors
  16. C-Terminal Peptides of Interleukin-6 Modulate the Expression of junB Protooncogene and the Production of Fibrinogen by HepG2 Cells
  17. Proteome Analysis by Three-Dimensional Protein Separation: Turnover of Cytosolic Proteins in Hepatocytes
  18. Structural Intermediates in the Putative Pathway from the Cellular Prion Protein to the Pathogenic Form
  19. Local Variability of the Phosphoglycerate Kinase-Triosephosphate Isomerase Fusion Protein from Thermotoga maritima MSB 8
  20. Epigenetics of Latent Epstein-Barr Virus Genomes: High Resolution Methylation Analysis of the Bidirectional Promoter Region of Latent Membrane Protein 1 and 2B Genes
  21. The Cytosine N4-Methyltransferase M.PvuII Also Modifies Adenine Residues
  22. Expression of the Human Menkes ATPase in Xenopus laevis Oocytes
https://doi.org/10.1515/BC.2001.080

Articles in the same Issue

  1. Vaccine Development: from Empirical Medicine to Molecularly Designed Therapy
  2. Dendritic Cells for Specific Cancer Immunotherapy
  3. Intracellular Bacteria as Targets and Carriers for Vaccination
  4. Bacteria-Mediated Transfer of Eukaryotic Expression Plasmids into Mammalian Host Cells
  5. Revealing the Potential of DNA-Based Vaccination: Lessons Learned from the Hepatitis B Virus Surface Antigen
  6. Progress toward a Malaria Vaccine: Efficient Induction of Protective Anti-Malaria Immunity
  7. Peptide Vaccines and Peptide Libraries
  8. Defined Synthetic Vaccines
  9. Antimicrobial Peptides: Properties and Applicability
  10. G-Quadruplex DNA Structures Variations on a Theme
  11. The Role of Heat Shock Proteins and Their Receptors in the Activation of the Immune System
  12. Transcriptional Repression Mediated by the KRAB Domain of the Human C2H2 Zinc Finger Protein Kox1/ZNF10 Does Not Require Histone Deacetylation
  13. Structure and Evolution of 4-Coumarate:Coenzyme A Ligase (4CL) Gene Families
  14. Inhibition of Hepatitis B Virus by Hammerhead Ribozyme Targeted to the Poly(A) Signal Sequence in Cultured Cells
  15. Chemical Accessibility of 18S rRNA in Native Ribosomal Complexes: Interaction Sites of mRNA, tRNA and Translation Factors
  16. C-Terminal Peptides of Interleukin-6 Modulate the Expression of junB Protooncogene and the Production of Fibrinogen by HepG2 Cells
  17. Proteome Analysis by Three-Dimensional Protein Separation: Turnover of Cytosolic Proteins in Hepatocytes
  18. Structural Intermediates in the Putative Pathway from the Cellular Prion Protein to the Pathogenic Form
  19. Local Variability of the Phosphoglycerate Kinase-Triosephosphate Isomerase Fusion Protein from Thermotoga maritima MSB 8
  20. Epigenetics of Latent Epstein-Barr Virus Genomes: High Resolution Methylation Analysis of the Bidirectional Promoter Region of Latent Membrane Protein 1 and 2B Genes
  21. The Cytosine N4-Methyltransferase M.PvuII Also Modifies Adenine Residues
  22. Expression of the Human Menkes ATPase in Xenopus laevis Oocytes
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2026 De Gruyter Brill
Downloaded on 11.1.2026 from https://www.degruyterbrill.com/document/doi/10.1515/BC.2001.080/html
Scroll to top button