Home Structure and Evolution of 4-Coumarate:Coenzyme A Ligase (4CL) Gene Families
Article
Licensed
Unlicensed Requires Authentication

Structure and Evolution of 4-Coumarate:Coenzyme A Ligase (4CL) Gene Families

  • Daniela Cukovica , Jürgen Ehlting , Jessica A. Van Ziffle and Carl J. Douglas
Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Volume 382 Issue 4

Abstract

The phenylpropanoid enzyme 4-coumarate:coenzyme A ligase (4CL) plays a key role in general phenylpropanoid metabolism. 4CL is related to a larger class of prokaryotic and eukaryotic adenylateforming enzymes and shares several conserved peptide motifs with these enzymes. In order to better characterize the nature of 4CL gene families in poplar, parsley, and tobacco, we used degenerate primers to amplify 4CL sequences from these species. In each species additional, divergent 4CL genes were found. Complete cDNA clones for the two new poplar 4CL genes were obtained, allowing examination of their expression patterns and determination of the substrate utilization profile of a xylemspecific isoform. Phylogenetic analysis of these genes and gene fragments confirmed previous results showing that 4CL proteins fall into two evolutionarily ancient subgroups . A comparative phylogenetic analysis of enzymes in the adenylate forming superfamily showed that 4CLs, luciferases, and acetate CoA ligases each form distinct clades within the superfamily. According to this analysis, four Arabidopsis 4CLlike genes identified from the Arabidopsis Genome Project are only distantly related to bona fide 4CLs or are more closely related to fatty acid CoA ligases, suggesting that the three Arabidopsis 4CL genes previously characterized represent the extent of the 4CL gene family in this species.

:
Published Online: 2005-06-01
Published in Print: 2001-04-27

Copyright © 2001 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Vaccine Development: from Empirical Medicine to Molecularly Designed Therapy
  2. Dendritic Cells for Specific Cancer Immunotherapy
  3. Intracellular Bacteria as Targets and Carriers for Vaccination
  4. Bacteria-Mediated Transfer of Eukaryotic Expression Plasmids into Mammalian Host Cells
  5. Revealing the Potential of DNA-Based Vaccination: Lessons Learned from the Hepatitis B Virus Surface Antigen
  6. Progress toward a Malaria Vaccine: Efficient Induction of Protective Anti-Malaria Immunity
  7. Peptide Vaccines and Peptide Libraries
  8. Defined Synthetic Vaccines
  9. Antimicrobial Peptides: Properties and Applicability
  10. G-Quadruplex DNA Structures Variations on a Theme
  11. The Role of Heat Shock Proteins and Their Receptors in the Activation of the Immune System
  12. Transcriptional Repression Mediated by the KRAB Domain of the Human C2H2 Zinc Finger Protein Kox1/ZNF10 Does Not Require Histone Deacetylation
  13. Structure and Evolution of 4-Coumarate:Coenzyme A Ligase (4CL) Gene Families
  14. Inhibition of Hepatitis B Virus by Hammerhead Ribozyme Targeted to the Poly(A) Signal Sequence in Cultured Cells
  15. Chemical Accessibility of 18S rRNA in Native Ribosomal Complexes: Interaction Sites of mRNA, tRNA and Translation Factors
  16. C-Terminal Peptides of Interleukin-6 Modulate the Expression of junB Protooncogene and the Production of Fibrinogen by HepG2 Cells
  17. Proteome Analysis by Three-Dimensional Protein Separation: Turnover of Cytosolic Proteins in Hepatocytes
  18. Structural Intermediates in the Putative Pathway from the Cellular Prion Protein to the Pathogenic Form
  19. Local Variability of the Phosphoglycerate Kinase-Triosephosphate Isomerase Fusion Protein from Thermotoga maritima MSB 8
  20. Epigenetics of Latent Epstein-Barr Virus Genomes: High Resolution Methylation Analysis of the Bidirectional Promoter Region of Latent Membrane Protein 1 and 2B Genes
  21. The Cytosine N4-Methyltransferase M.PvuII Also Modifies Adenine Residues
  22. Expression of the Human Menkes ATPase in Xenopus laevis Oocytes
Downloaded on 23.9.2025 from https://www.degruyterbrill.com/document/doi/10.1515/BC.2001.076/html
Scroll to top button