Assessment of biochemical changes in normal and diabetic rats treated by phenolic enriched extracts of Juglans regia L. barks

Nor-El-Houda Benahmed; Mokhtar Benabderrahmane; Rachid Azzi; Faiza Mouderas; Djoher Aberkane; Abdesselam Hichem Mai; Amel Bendiabdellah

doi:10.1515/jcim-2021-0083

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Article

Assessment of biochemical changes in normal and diabetic rats treated by phenolic enriched extracts of Juglans regia L. barks

Nor-El-Houda Benahmed , Mokhtar Benabderrahmane , Rachid Azzi , Faiza Mouderas , Djoher Aberkane , Abdesselam Hichem Mai and Amel Bendiabdellah

Published/Copyright: May 19, 2021

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From the journal Journal of Complementary and Integrative Medicine Volume 19 Issue 4

Abstract

Objectives

Juglans regia L. is largely used by diabetic population, but its bark is scarcely investigated. The major aim of this study was to investigate the effect of the hydro-methanolic extract and fractions of J. regia L. barks on normoglycemic and diabetic rats through the evaluation of some biochemical parameters.

Methods

Phenolic enriched extracts were investigated for their effect on normoglycemic and diabetic rats through the determination of fasting blood glucose levels in short- and long-term. As well as plasma total cholesterol, triglycerides, insulin, urea, creatinine, alanine amino transferase (ALT) and aspartate amino transferase (AST) levels were measured after 28 days of treatment. Furthermore, the phytochemical composition was assessed by standard methods.

Results

Among the different extracts tested, the n-Butanol fraction presented the highest amounts of total phenolic, total flavonoids and flavonol contents (745 ± 0.6 mg GAE g⁻¹, 162 ± 0.78 mg CE g⁻¹ and 56.02 ± 0.92 mg QE g⁻¹, respectively) and it found to be the most effective to decrease plasma total cholesterol, triglycerides and FGLs in treated diabetic rats in both short- and long-term treatment, mainly the dose 200 mg/kg after 28 days of treatment which found to be more effective than glibenclamide. This fraction also revealed pronounced improvement in liver and renal profiles.

Conclusions

J. regia L. barks had an important antidiabetic role similar to the reference glibenclamide. This may be related to the high contents of phenolic compounds.

Keywords: biochemical parameters; experimental diabetes mellitus; Juglans regia L. barks; phenolic contents

Corresponding author: Prof. Rachid Azzi, Professor in Biochemistry, Laboratoire Affiliation: Antibiotiques Antifongiques: Physico-chimie, Synthèse et Activité Biologique, Faculté des Sciences de la Nature et de la Vie et des Sciences de la Terre et de l’univers, Université de Tlemcen, Tlemcen, Algeria; and Postal Address: Department of Biology, Faculty of Natural Sciences and Life Sciences of the Earth and the Universe, University of Tlemcen, BP 119, Tlemcen, Algeria, Phone: +213(0)5 49 528 416, E-mail: rachid.azzi@univ-tlemcen.dz

Research funding: None declared.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Competing interests: The authors declare that there is no conflict of interest.
Informed consent: Not applicable.
Ethical approval: The experiments were carried out under ethical conditions and were approved by the Ethical Committee for animals of Djilali Liabes University.

References

1. Hawash, M, Jaradat, N, Elaraj, J, Hamdan, A, Lebdeh, SA, Halawa, T. Evaluation of the hypoglycemic effect of seven wild folkloric edible plants from Palestine. J Compl Integr Med 2019;17:20190032. https://doi.org/10.1515/jcim-2019-0032.Search in Google Scholar PubMed

2. Bowman, P, Sulen, A, Barbetti, F, Beltrand, J, Svalastoga, P, Codner, E, et al.. Effectiveness and safety of long-term treatment with sulfonylureas in patients with neonatal diabetes due to KCNJ11 mutations: an international cohort study. Lancet Diabetes Endocrinol 2018;6:637–46. https://doi.org/10.1016/S2213-8587(18)30106-2.Search in Google Scholar PubMed PubMed Central

3. Ghauri, AO, Ahmad, S, Rehman, T. In vitro and in vivo anti-diabetic activity of Citrullus colocynthis pulpy flesh with seeds hydro-ethanolic extract. J Compl Integr Med 2020;17:20180228. https://doi.org/10.1515/jcim-2018-0228.Search in Google Scholar PubMed

4. Al-Awaida, WJ, Sharab, AS, Al-Ameer, HJ. Ayoub, N. Effect of simulated microgravity on the antidiabetic properties of wheatgrass (Triticum aestivum) in streptozotocin-induced diabetic rats. NPJ Microgravity 2020;6:6. https://doi.org/10.1038/s41526-020-0096-x.Search in Google Scholar PubMed PubMed Central

5. Mazokopakis, EE, Starakis, IK. Recommendations for diagnosis and management of metformin-induced vitamin B12 (Cbl) deficiency. Diabetes Res Clin Pract 2012;97:359–67. https://doi.org/10.1016/j.diabres.2012.06.001.Search in Google Scholar PubMed

6. Cheynier, V. Phenolic compounds: from plants to foods. Phytochem Rev 2012;11:153–77. https://doi.org/10.1007/s11101-012-9242-8.Search in Google Scholar

7. Cao, H, Ou, J, Chen, L, Zhang, Y, Szkudelski, T, Delmas, D, et al.. Dietary polyphenols and type 2 diabetes: human study and clinical trials. Crit Rev Food Sci Nutr 2019;59:3371–9. https://doi.org/10.1080/10408398.2018.1492900.Search in Google Scholar PubMed

8. Zhang, S, Xue, R, Hu, R. The neuroprotective effect and action mechanism of polyphenols in diabetes mellitus-related cognitive dysfunction. Eur J Nutr 2020;59:1295–311. https://doi.org/10.1007/s00394-019-02078-2.Search in Google Scholar PubMed

9. Kang, GG, Francis, N, Hill, R, Waters, D, Blanchard, C, Santhakumar, AB. Dietary polyphenols and gene expression in molecular pathways associated with type 2 diabetes mellitus: a review. Int J Mol Sci 2019;21:140. https://doi.org/10.3390/ijms21010140.Search in Google Scholar PubMed PubMed Central

10. Delavis, H, Mohammadi, J, Ghalamfarsa, G, Mohammadi, B, Farhadi, N. A review study on phytochemistry and pharmacology applications of Juglans regia plant. Pharmacogn Rev 2017;11:145–52. https://doi.org/10.4103/phrev.phrev_10_17.Search in Google Scholar PubMed PubMed Central

11. Delaviz, H, Mohammadi, J, Ghalamfarsa, G, Mohammadi, B, Farhadi, N. A review study on phytochemistry and pharmacology applications of Juglans regia plant. Pharmacogn Rev 2017;11:145–52. https://doi.org/10.4103/phrev.phrev_10_17.Search in Google Scholar PubMed PubMed Central

12. Trease, GE, Evans, WC. Phenols and phenolic glycosides. In: Textbook of pharmacognosy. London, UK: Balliese, Tindall and Co Publishers; 1989, vol 12:343–83 pp.Search in Google Scholar

13. Harborne, J. Phytochemical methods. In: A guide to modern techniques of plant analysis, 3rd ed. UK: Chapman and Hall Thomson Science; 1998:203–34 pp.Search in Google Scholar

14. Vermerris, W, Nicholson, R Isolation and identification of phenolic compounds, phenolic compound biochemistry. Dordrecht: Springer; 2006, vol 35:151–91 pp.10.1007/978-1-4020-5164-7_4Search in Google Scholar

15. Zhishen, J, Mengcheng, T, Jianming, W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555–9. https://doi.org/10.1016/s0308-8146(98)00102-2.Search in Google Scholar

16. Yermakov, AI, Arasimov, VV, Yarosh, NP. Methods of biochemical analysis of plants. Leningrad, Russia: Agropromizdat; 1987.Search in Google Scholar

17. Ganesan, R, Mathuram, V. Identification of bioactive constituents in Coldenia procumbens L. and its antidiabetic activity against streptozotocin induced Wistar albino rats. J Compl Integr Med 2020;17:20190085. https://doi.org/10.1515/jcim-2019-0085.Search in Google Scholar PubMed

18. Adams, DM, Yakubu, MT. Aqueous extract of Digitariaexilis grains ameliorate diabetes in streptozotocin-induced diabetic male Wistar rats. J Ethnopharmacol 2020;249:112383. https://doi.org/10.1016/j.jep.2019.112383.Search in Google Scholar PubMed

19. Noumi, E, Hajlaoui, H, Trabelsi, N, Ksouri, R, Bakhrouf, A, Snoussi, M. Antioxidant activities and RP-HPLC identification of polyphenols in the ethyl acetate extract of Tunisian Juglans regia L treated barks. J Med Plants Res 2012;6:1468–75.10.5897/JMPR11.1405Search in Google Scholar

20. Azhar, N, Khan, MQ, Riaz, M, Hussain, MA, Dar, M, Shaheen, H, et al.. Measurement of toxic metal contamination, phenolic and flavonoid contents, anticancer, antimicrobial and antioxidant activities of dietary and medicinal plant Juglans regia L. Appl Ecol Environ Res 2018;16:2307–21. https://doi.org/10.15666/aeer/1603_23072321.Search in Google Scholar

21. Mouderas, F, Lahfa, FB, Mezouar, D, Benahmed, NEH. Valorization and identification of bioactive compounds of a spiceAmmodaucusleucotrichus. Adv Tradit Med 2020;20:159–68. https://doi.org/10.1007/s13596-019-00390-0.Search in Google Scholar

22. Mahdi, S, Azzi, R, Lahfa, FB. Evaluation of in vitro α-amylase and α-glucosidase inhibitory potential and hemolytic effect of phenolic enriched fractions of the aerial part of Salvia officinalis L. Diabetes Metab Syndr 2020;14:689–94. https://doi.org/10.1016/j.dsx.2020.05.002.Search in Google Scholar PubMed

23. Seedevi, P, Ganesan, R, Moovendhan, M, Mohan, K, Sivasankar, P, Loganathan, S, et al.. Anti-diabetic activity of crude polysaccharide and rhamnose-enriched polysaccharide from G. lithophila on Streptozotocin (STZ)-induced in Wistar rats. Sci Rep 2020;10:556. https://doi.org/10.1038/s41598-020-57486-w.Search in Google Scholar PubMed PubMed Central

24. Rašeta, M, Popović, M, Čapo, I, Stilinović, N, Vukmirović, S, Milošević, B, et al.. Antidiabetic effect of two different Ganoderma species tested in alloxan diabetic rats. RSC Adv 2020;10:10382–93.10.1039/C9RA10158FSearch in Google Scholar PubMed PubMed Central

25. Jelodar, G, Mohammadi, M, Akbari, A, Nazifi, S. Cyclohexane extract of walnut leaves improves indices of oxidative stress, total homocysteine and lipids profiles in streptozotocin-induced diabetic rats. Physiol Rep 2020;8:4348. https://doi.org/10.14814/phy2.14348.Search in Google Scholar PubMed PubMed Central

26. Teimori, M, Kouhsari, MS, Ghafarzadegan, R, Hajiaghaee, R. Study of hypoglycemic effect of Juglans regia leaves and its mechanism. J Med Plants 2010;9:57–65.Search in Google Scholar

Received: 2021-02-23

Accepted: 2021-04-29

Published Online: 2021-05-19

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Articles in the same Issue

https://doi.org/10.1515/jcim-2021-0083

Keywords for this article

biochemical parameters; experimental diabetes mellitus; Juglans regia L. barks; phenolic contents