Startseite Acute and sub-acute toxicity assessment of the standardized extract of Sanguisorba minor in vivo
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Acute and sub-acute toxicity assessment of the standardized extract of Sanguisorba minor in vivo

  • Legha Ansari , Mohammad Reza Mahdinezhad , Hassan Rakhshandeh , Azar Hosseini , Samaneh Boroumand Noughabi , Najibeh Gholami und Arezoo Rajabian EMAIL logo
Veröffentlicht/Copyright: 6. Dezember 2021
Journal of Complementary and Integrative Medicine
Aus der Zeitschrift Journal of Complementary and Integrative Medicine Band 19 Heft 4

Abstract

Although Sanguisorba minor has been used as herbal medicine, no study has ever examined its potential toxicity. This study investigated acute and subacute toxicities of S. minor hydroalcoholic extract (SE). In the acute toxicity test, a single oral dose (300, 2,000, and 3,000 mg/kg) of SE was given to mice. The oral administration of SE (100, 200, and 400 mg/kg for 4 weeks) was performed to evaluate subacute toxicity. After the treatments, neurobehavioral, histopathology, hematological, and biochemical parameters were monitored. In vitro cytotoxicity was also assessed. Moreover, high-performance liquid chromatography fingerprint was done for the standardization of SE. The no-observed-adverse-effect level of SE was up to 2,000 mg/kg, and the LD50 of the prepared extract was over 3,000 mg/kg. The rats exposed to the extract did not show any marked change in their body weight. The extract at used doses did not affect neuromuscular coordination. According to the hematological, biochemical, and histological examinations, no significant treatment-related adverse effect of the extract was observed, even at 400 mg/kg. Only 48 h exposure to 400 μg/mL of SE reduced the viability of PC12 cells. The findings revealed that this plant could be well-tolerated, regarded safe, and used as herbal medicine.

Keywords: acute and subacute oral toxicities; hematology; herbal medicine; histopathology; Sanguisorba minor ; serum biochemistry analysis
Corresponding author: Arezoo Rajabian, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; and Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran, Phone: +98 38828265, Fax: +98 51 38828567, E-mail:

Funding source: Mashhad University of Medical Sciences

Award Identifier / Grant number: 990641

Acknowledgments

This study was originated from a MD project of Najibeh Gholami.

  1. Research funding: This project was financially supported by Vice Chancellery for Research and Technology, Mashhad University of Medical Sciences with Grant No. 990641.

  2. Author contributions: Arezoo Rajabian: Conceptualization, Methodology, providing revision of the manuscript. Legha Ansari, Mohammad Reza Mahdinezhad, Najibeh Gholami: Acquisition of data, Data curation, Writing-Original draft preparation. Arezoo Rajabian, Samaneh Boroumand Noughabi: Analysis and/or interpretation of data, Investigation. Arezoo Rajabian, Hassan Rakhshandeh: Supervision: Arezoo Rajabian, Azar Hosseini: Reviewing and Editing.

  3. Competing interests: The authors declare that they have no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethics approval: All procedures are in agreement with Guidelines for Care and Use of Laboratory Animals, National Institutes of Health, and confirmed by the Institutional Animal Care and Use Committee of MUMS (Ethical code: IR.MUMS.REC.1399.388).

  6. Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Received: 2021-08-27
Accepted: 2021-11-15
Published Online: 2021-12-06

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

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  2. Reviews
  3. A preliminary systematic review and meta-analysis on the effects of heart rate variability biofeedback on heart rate variability and respiration of athletes
  4. The potential positive epigenetic effects of various mind-body therapies (MBTs): a narrative review
  5. The effect of Chlorella vulgaris on obesity related metabolic disorders: a systematic review of randomized controlled trials
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  7. The effect of the herbal medicine on severity of cyclic mastalgia: a systematic review and meta-analysis
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  18. Quantification of anacardic acid, the toxic component in raw and purified samples of Semecarpus anacardium L. by Siddha purification processes
  19. Molecular docking and molecular dynamics approach to identify potential compounds in Huperzia squarrosa for treating Alzheimer’s disease
  20. Evaluation of ethanol extracts from three species of Artocarpus as natural gastroprotective agents: in vivo and histopathological studies
  21. Attenuation of cisplatin induced myelosuppression by methanol extract of Cedrus deodara in Wistar rats
  22. Acute and sub-acute toxicity assessment of the standardized extract of Sanguisorba minor in vivo
  23. Efficacy of lettuce seed syrup on insomnia in patients with breast cancer: a pilot double blind randomized placebo controlled clinical trial
  24. The effect of aromatherapy with rose essential oil on apparent anxiety in patients with myocardial infarction
  25. Effect of Jyoti-Trataka on intraocular pressure, autonomic control, and blood glucose in diabetic patients with high-tension primary open-angle glaucoma: a randomized-controlled trial
  26. Efficacy of Ḥammām-i-yābis (dry bath) in metabolic syndrome: a single arm, open-labelled clinical trial
  27. Effect of Ḥijāma (wet cupping), Dalk (massage) and Bukhūr (medicated steam) in amelioration of Waja al-Zahr (non-specific low back pain) – an open prospective clinical trial
  28. Effect of yoga on cardiovascular functions and psychological aspects of people on public service-related work: an exploratory study
https://doi.org/10.1515/jcim-2021-0391
Schlagwörter für diesen Artikel
acute and subacute oral toxicities; hematology; herbal medicine; histopathology; Sanguisorba minor; serum biochemistry analysis

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. A preliminary systematic review and meta-analysis on the effects of heart rate variability biofeedback on heart rate variability and respiration of athletes
  4. The potential positive epigenetic effects of various mind-body therapies (MBTs): a narrative review
  5. The effect of Chlorella vulgaris on obesity related metabolic disorders: a systematic review of randomized controlled trials
  6. Biological and medicinal application of Cucumis sativus Linn. – review of current status with future possibilities
  7. The effect of the herbal medicine on severity of cyclic mastalgia: a systematic review and meta-analysis
  8. Research Articles
  9. Chemical compositions, antibacterial, antifungal and cytotoxic effects of Alhagi mannifera five extracts
  10. Anticonvulsive and anti-epileptogenesis effects of Echinacea purpurea root extract, an involvement of CB2 receptor
  11. Fermented maize slurry (Ogi) and its supernatant (Omidun) mitigate elevated intraocular pressure by modulating BDNF expression and glial plasticity in the retina-gut axis of glaucomatous rats
  12. Levetiracetam exposure during prenatal and postnatal period induces cognitive decline in rat offsprings, not completely prevented by Bacopa monnieri
  13. Antibiofilm action of Persea americana glycolic extract over Acinetobacter baumannii and absence of toxicity in Galleria mellonella
  14. Validation of Unani concept of Abadāl-i-Adwiya (drug substitution) by physicochemical standardization and hepatoprotective activity of Aristolochia rotunda Linn. and its substitute Curcuma Zedoaria Rosc. in albino Wistar rats
  15. Cinnamon oil as a co-chemotherapy agent through inhibition of cell migration and MMP-9 expression on 4T1 cells
  16. Assessment of biochemical changes in normal and diabetic rats treated by phenolic enriched extracts of Juglans regia L. barks
  17. Influence of Clerodendrum volubile leaf extract on doxorubicin-induced toxicity and inhibition of carbonyl reductase mediated metabolism
  18. Quantification of anacardic acid, the toxic component in raw and purified samples of Semecarpus anacardium L. by Siddha purification processes
  19. Molecular docking and molecular dynamics approach to identify potential compounds in Huperzia squarrosa for treating Alzheimer’s disease
  20. Evaluation of ethanol extracts from three species of Artocarpus as natural gastroprotective agents: in vivo and histopathological studies
  21. Attenuation of cisplatin induced myelosuppression by methanol extract of Cedrus deodara in Wistar rats
  22. Acute and sub-acute toxicity assessment of the standardized extract of Sanguisorba minor in vivo
  23. Efficacy of lettuce seed syrup on insomnia in patients with breast cancer: a pilot double blind randomized placebo controlled clinical trial
  24. The effect of aromatherapy with rose essential oil on apparent anxiety in patients with myocardial infarction
  25. Effect of Jyoti-Trataka on intraocular pressure, autonomic control, and blood glucose in diabetic patients with high-tension primary open-angle glaucoma: a randomized-controlled trial
  26. Efficacy of Ḥammām-i-yābis (dry bath) in metabolic syndrome: a single arm, open-labelled clinical trial
  27. Effect of Ḥijāma (wet cupping), Dalk (massage) and Bukhūr (medicated steam) in amelioration of Waja al-Zahr (non-specific low back pain) – an open prospective clinical trial
  28. Effect of yoga on cardiovascular functions and psychological aspects of people on public service-related work: an exploratory study
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