Startseite Influence of Clerodendrum volubile leaf extract on doxorubicin-induced toxicity and inhibition of carbonyl reductase mediated metabolism
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Influence of Clerodendrum volubile leaf extract on doxorubicin-induced toxicity and inhibition of carbonyl reductase mediated metabolism

  • Olorunfemi R. Molehin EMAIL logo , Kehinde A. Idowu , Ayonposi B. Olaoye , Aderonke E. Fakayode und Oluwatumininu O. Adesua
Veröffentlicht/Copyright: 10. Mai 2021
Journal of Complementary and Integrative Medicine
Aus der Zeitschrift Journal of Complementary and Integrative Medicine Band 19 Heft 4

Abstract

Objectives

Doxorubicin (DOX) is a commonly used chemotherapeutic drug. However, its non-target organ toxicities pose a serious problem. This study is to assess the protective role of Clerodendrum volubile leaf extract (CVE) against DOX-induced toxicities in rats. In addition, the inhibitory activities of three phytochemical compounds (Rutin, Gallic acid and Rosmarinic acid) from CVE against Carbonyl reductase 1 (CBR1) were examined.

Methods

Rats were randomly divided into 5 groups: (a) Control group rats were given 0.9% NaCl as vehicle, (b) DOX group: A single dose of DOX (25 mg/kg; i.p.) was administered and rats were sacrificed 4 days after DOX injection, while groups (c–e) CVE-treated DOX rat groups were given 125, 250 and 500 mg/kg body weight of extracts orally for 12 consecutive days; 8 days before, and 4 days after the DOX administration. Computational techniques were used to determine the inhibitory activities of the compounds against CBR1.

Results

DOX intoxication caused a significant increase (p<0.05) in serum marker enzymes: ALT, AST, ALP, LDH, CK activities. The levels of liver and heart tissues antioxidant parameters: GPx, SOD, CAT, and GSH were significantly (p<0.05) decreased in DOX-intoxicated rats with concomitant elevation of malondialdehyde levels. Pretreatment with CVE reversed the above trends. From the structural analysis, Rutin and RSA exhibited the highest binding free energies against CBR1, and also exhibited structural stability when bound with CBR1.

Conclusions

Our study indicates the protective effect of CVE when used in combination with doxorubicin thus improving its chemotherapeutic application via inhibition of CBR-mediated metabolism.

Keywords: antioxidant; carbonyl reductase; Clerodendrum volubile ; doxorubicin; molecular docking; oxidative stress
Corresponding author: Olorunfemi R. Molehin, PhD, AMRSC, Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti, P.M.B. 5363, Ado-Ekiti, 360001, Nigeria, Phone: +234 803 462 1267, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All the authors read and approved this submitted manuscript. The authors have also accepted responsibility for the entire content of this manuscript.

  3. Competing interests: The funding organization (s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

  4. Informed consent: Not applicable.

  5. Ethical approval: These ethic regulations were diligently followed during the experiment. This study also conforms to our institutional protocols of The Research and Ethics Committee, of the Office of Research and Development, Ekiti State University, Ado-Ekiti, Nigeria (ORD/AD/EAC/19/0026).

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Received: 2020-07-27
Accepted: 2021-02-15
Published Online: 2021-05-10

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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  17. Influence of Clerodendrum volubile leaf extract on doxorubicin-induced toxicity and inhibition of carbonyl reductase mediated metabolism
  18. Quantification of anacardic acid, the toxic component in raw and purified samples of Semecarpus anacardium L. by Siddha purification processes
  19. Molecular docking and molecular dynamics approach to identify potential compounds in Huperzia squarrosa for treating Alzheimer’s disease
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  23. Efficacy of lettuce seed syrup on insomnia in patients with breast cancer: a pilot double blind randomized placebo controlled clinical trial
  24. The effect of aromatherapy with rose essential oil on apparent anxiety in patients with myocardial infarction
  25. Effect of Jyoti-Trataka on intraocular pressure, autonomic control, and blood glucose in diabetic patients with high-tension primary open-angle glaucoma: a randomized-controlled trial
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  27. Effect of Ḥijāma (wet cupping), Dalk (massage) and Bukhūr (medicated steam) in amelioration of Waja al-Zahr (non-specific low back pain) – an open prospective clinical trial
  28. Effect of yoga on cardiovascular functions and psychological aspects of people on public service-related work: an exploratory study
https://doi.org/10.1515/jcim-2020-0231
Schlagwörter für diesen Artikel
antioxidant; carbonyl reductase; Clerodendrum volubile; doxorubicin; molecular docking; oxidative stress

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. A preliminary systematic review and meta-analysis on the effects of heart rate variability biofeedback on heart rate variability and respiration of athletes
  4. The potential positive epigenetic effects of various mind-body therapies (MBTs): a narrative review
  5. The effect of Chlorella vulgaris on obesity related metabolic disorders: a systematic review of randomized controlled trials
  6. Biological and medicinal application of Cucumis sativus Linn. – review of current status with future possibilities
  7. The effect of the herbal medicine on severity of cyclic mastalgia: a systematic review and meta-analysis
  8. Research Articles
  9. Chemical compositions, antibacterial, antifungal and cytotoxic effects of Alhagi mannifera five extracts
  10. Anticonvulsive and anti-epileptogenesis effects of Echinacea purpurea root extract, an involvement of CB2 receptor
  11. Fermented maize slurry (Ogi) and its supernatant (Omidun) mitigate elevated intraocular pressure by modulating BDNF expression and glial plasticity in the retina-gut axis of glaucomatous rats
  12. Levetiracetam exposure during prenatal and postnatal period induces cognitive decline in rat offsprings, not completely prevented by Bacopa monnieri
  13. Antibiofilm action of Persea americana glycolic extract over Acinetobacter baumannii and absence of toxicity in Galleria mellonella
  14. Validation of Unani concept of Abadāl-i-Adwiya (drug substitution) by physicochemical standardization and hepatoprotective activity of Aristolochia rotunda Linn. and its substitute Curcuma Zedoaria Rosc. in albino Wistar rats
  15. Cinnamon oil as a co-chemotherapy agent through inhibition of cell migration and MMP-9 expression on 4T1 cells
  16. Assessment of biochemical changes in normal and diabetic rats treated by phenolic enriched extracts of Juglans regia L. barks
  17. Influence of Clerodendrum volubile leaf extract on doxorubicin-induced toxicity and inhibition of carbonyl reductase mediated metabolism
  18. Quantification of anacardic acid, the toxic component in raw and purified samples of Semecarpus anacardium L. by Siddha purification processes
  19. Molecular docking and molecular dynamics approach to identify potential compounds in Huperzia squarrosa for treating Alzheimer’s disease
  20. Evaluation of ethanol extracts from three species of Artocarpus as natural gastroprotective agents: in vivo and histopathological studies
  21. Attenuation of cisplatin induced myelosuppression by methanol extract of Cedrus deodara in Wistar rats
  22. Acute and sub-acute toxicity assessment of the standardized extract of Sanguisorba minor in vivo
  23. Efficacy of lettuce seed syrup on insomnia in patients with breast cancer: a pilot double blind randomized placebo controlled clinical trial
  24. The effect of aromatherapy with rose essential oil on apparent anxiety in patients with myocardial infarction
  25. Effect of Jyoti-Trataka on intraocular pressure, autonomic control, and blood glucose in diabetic patients with high-tension primary open-angle glaucoma: a randomized-controlled trial
  26. Efficacy of Ḥammām-i-yābis (dry bath) in metabolic syndrome: a single arm, open-labelled clinical trial
  27. Effect of Ḥijāma (wet cupping), Dalk (massage) and Bukhūr (medicated steam) in amelioration of Waja al-Zahr (non-specific low back pain) – an open prospective clinical trial
  28. Effect of yoga on cardiovascular functions and psychological aspects of people on public service-related work: an exploratory study
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