Validation of Unani concept of Abadāl-i-Adwiya (drug substitution) by physicochemical standardization and hepatoprotective activity of Aristolochia rotunda Linn. and its substitute Curcuma Zedoaria Rosc. in albino Wistar rats

Athar Parvez Ansari; Sumyyah Hasina Sana; Mohammad Younis Dar; Pankaj Goswami; N. Zaheer Ahmed

doi:10.1515/jcim-2020-0378

Article

Validation of Unani concept of Abadāl-i-Adwiya (drug substitution) by physicochemical standardization and hepatoprotective activity of Aristolochia rotunda Linn. and its substitute Curcuma Zedoaria Rosc. in albino Wistar rats

Athar Parvez Ansari , Sumyyah Hasina Sana , Mohammad Younis Dar , Pankaj Goswami and N. Zaheer Ahmed

Published/Copyright: July 15, 2021

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From the journal Journal of Complementary and Integrative Medicine Volume 19 Issue 4

Abstract

Objectives

To validate the concept of abadāl-i-adwiya (drug substitution) by evaluation of physicochemical standardization and hepatoprotective activity of Aristolochia rotunda & its substitute, Curcuma Zedoaria in albino Wistar rats.

Methods

Physicochemical standardization by estimation of moisture content, ash values and extractive values were carried out using standard methods. Hepatotoxicity was induced in albino Wistar rats using CCl₄ 1 mL/kg s. c. on alternate day for 14 days. Group I was served as Plain control and Group II as Negative control. Group III was administered silymarin 50 mg/kg p. o. while Group IV received HAE of A. rotunda 89.64 mg/kg p. o., and Group V was administered HAE of C. Zedoaria 45.73 mg/kg p. o. At the end of the study, serum bilirubin, AST (SGOT), ALT (SGPT) and ALP were estimated. The histopathology of liver was also carried out.

Results

The physicochemical parameters of both test drugs viz. moisture content, total ash, acid insoluble ash and water soluble ash were found within normal limit. The total serum bilirubin, direct bilirubin, AST (SGOT), ALT (SGPT) levels were significantly decreased in Test groups A and B when compared to the Negative and Standard controls. The microscopic examination of liver collected from animals of Group IV and Group V revealed significant recovery from hepatic toxicity compared to the Negative control.

Conclusions

The study experimentation has revealed that C. Zedoaria may be used as a substitute for A. rotunda in the treatment of liver diseases. However, the outcome has to be further corroborated with phytochemical evaluation and clinical trials of both the drugs. Furthermore, the concept of drug substitute in Unani system of medicine is also validated in the light of above study.

Keywords: Abadāl-i-Adwiya ; Aristolochia rotunda ; Curcuma Zedoaria ; drug substitution; hepatoprotective; Unani medicine

Corresponding author: Athar Parvez Ansari, Regional Research Institute of Unani Medicine, University of Kashmir Naseem Bagh Campus, Habak, Srinagar, Jammu & Kashmir 190006, India, Phone: +91 9952961233, E-mail: aatharparvez@gmail.com

Funding source: Central Council for research in Unani Medicine, Ministry of AYUSH, Govt. of India

Acknowledgments

The authors are indebted to the Ministry of AYUSH, Govt. of India and Prof. Asim Ali Khan, Director General, CCRUM, New Delhi, India for providing financial support to the study. The authors are grateful to Dr. Seema Akbar, Assistant Director (Chemistry)/Head of the Institute, Regional Research Institute of Unani Medicine, Srinagar, Jammu & Kashmir, India for providing entire facilities required for this study and for her constant support and keen interest in this research work. We are also thankful to Dr. Huzaifa Ansari, Dr. Shahabuddin, Dr. Juwaria Shamim, Dr. Abu Nasir, P. G. Scholars, Dept. of Unani Pharmacology, RRIUM, Srinagar for their continuous assistance during the preclinical trial.

Research funding: This research work was funded by the Central Council for Research in Unani Medicine, Ministry of AYUSH, Govt. of India.
Author contributions: Athar Parvez Ansari and Sumyyah Hasina Sana: Conceptualization, methodology, data curation, investigation, writing original draft. Mohammad Younis Dar and Pankaj Goswami: investigation, review and editing. Dr. N. Zaheer Ahmed: Reviewing and editing of the manuscript.
Competing interests: Authors state no conflict of interest.
Informed consent: Not applicable
Ethical approval: Not applicable

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Received: 2020-09-12

Accepted: 2021-07-04

Published Online: 2021-07-15

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Articles in the same Issue

https://doi.org/10.1515/jcim-2020-0378

Keywords for this article

Abadāl-i-Adwiya; Aristolochia rotunda; Curcuma Zedoaria; drug substitution; hepatoprotective; Unani medicine