Startseite DNA barcoding of bats (Chiroptera) from the Colombian northern region
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DNA barcoding of bats (Chiroptera) from the Colombian northern region

  • Álvaro J. Benítez ORCID logo , Dina Ricardo-Caldera ORCID logo EMAIL logo , María Atencia-Pineda , Jesús Ballesteros-Correa , Julio Chacón-Pacheco ORCID logo und Richard Hoyos-López
Veröffentlicht/Copyright: 13. Mai 2021
Mammalia
Aus der Zeitschrift Mammalia Band 85 Heft 5

Abstract

Bats are mammals of great ecological and medical importance, which have associations with different pathogenic microorganisms. DNA barcoding is a tool that can expedite species identification using short DNA sequences. In this study, we assess the DNA barcoding methodology in bats from the Colombian Northern region, specifically in the Córdoba department. Cytochrome oxidase subunit I (COI) gene sequences of nine bat species were typified, and their comparison with other Neotropic samples revealed that this marker is suitable for individual species identification, with ranges of intra-species variation from 0.1 to 0.9%. Bat species clusters are well supported and differentiated, showing average genetic distances ranging from 3% between Artibeus lituratus and Artibeus planirostris, up to 27% between Carollia castanea and Molossus molossus. C. castanea and Glossophaga soricina show geographical structuring in the Neotropic. The findings reported in this study confirm DNA barcoding usefulness for fast species identification of bats in the region.

Keywords: Chiroptera; Cytochrome oxidase subunit I (COI); Glossophaga soricina ; taxonomy
Corresponding author: Dina Ricardo-Caldera, Facultad de Ciencias de la Salud, Grupo de investigación en Enfermedades Tropicales y Resistencia Bacteriana, Universidad del Sinú, Calle 38 Cra. 1 W, Barrio Juan XXIII, 230001 Montería, Córdoba, Colombia, E-mail:

Funding source: Córdoba Government, the General System of Royalties

Award Identifier / Grant number: 754/2013

Funding source: Universidad del Sinú – Elías Bechara Zainúm

Funding source: Universidad de Córdoba

Acknowledgments

The authors would like to thank the Molecular Biology and Biomedical Science Laboratory from Universidad del Sinú, for its support in accomplishing the molecular protocols, as well as the people who collaborated in the field phase. The authors would like to give special thanks to Josef Bryja, Héctor Ramírez, and two anonymous reviewers for their valuable comments on the manuscript.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was funded by the Córdoba Government, the General System of Royalties (SGR, for its acronym in Spanish), Colombia (grant no. 754/2013), Universidad del Sinú – Elías Bechara Zainúm (UNISINÚ) and Universidad de Córdoba.

  3. Conflict of interest statement: The authors declare that they have no conflicts of interest regarding this article.

  4. Research ethics: All the methods used in this study were approved by the Health Sciences Faculty Ethics’ Committee from Universidad del Sinú (Act No 01 of 2017), following the ethical guidelines established by the American Society of Mammalogists for specimens euthanasia (Sikes and The Animal Care and Use Committee of the American Society of Mammalogists 2016). The collection was carried out under the permission of the National Authority of Environmental Licenses for Universidad de Córdoba, through resolution 00914 (August 4th, 2017), to conduct scientific research without commercial purposes in wild animals. Specimens were deposited in the zoological collection from Universidad de Córdoba (CZUC). All sequences were registered on GenBank under the following accession numbers: from MK749466 to MK749564.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/mammalia-2020-0138).

Received: 2020-09-19
Accepted: 2021-04-09
Published Online: 2021-05-13
Published in Print: 2021-09-27

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Conservation
  3. Ansorge’s cusimanse in Angola: 100 years apart, new records contribute to the species known range
  4. Abundance of the exploited red-rumped agouti (Dasyprocta leporina Rodentia: Dasyproctidae) on the island of Trinidad
  5. Biogeography
  6. The roles of possible geographic barriers and geological events on the phylogeographic structure of the Eastern broad toothed field mouse (Apodemus mystacinus)
  7. First record of Blanford’s Fox Vulpes cana (Blanford, 1877) from Iraq
  8. New records and southern range extension of the Annamite striped rabbit Nesolagus timminsi in Vietnam
  9. Ecology
  10. A pilot study of the use of dry dog food as an alternative attractant in mesocarnivore studies
  11. Seasonal diet composition of Himalayan goral (Naemorhedus goral) in Kajinag National Park, Jammu and Kashmir, India
  12. Seasonal and daily activity patterns of mammals in the colony of great cormorants
  13. First North African record of a melanistic common genet (Genetta genetta Linnaeus, 1758)
  14. Albinism in Brazilian common opossums (Didelphis aurita)
  15. Evolutionary biology
  16. Brain size evolution in small mammals: test of the expensive tissue hypothesis
  17. Taxonomy/phylogeny
  18. DNA barcoding of bats (Chiroptera) from the Colombian northern region
  19. Morphology and genetics concur that Anoura carishina is a synonym of Anoura latidens (Chiroptera, Glossophaginae)
  20. Taxonomy of Ctenomys (Rodentia: Ctenomyidae) in northwestern Patagonia, Argentina: the occurrence of the “mendocinus” lineage
https://doi.org/10.1515/mammalia-2020-0138
Schlagwörter für diesen Artikel
Chiroptera; Cytochrome oxidase subunit I (COI); Glossophaga soricina; taxonomy

Artikel in diesem Heft

  1. Frontmatter
  2. Conservation
  3. Ansorge’s cusimanse in Angola: 100 years apart, new records contribute to the species known range
  4. Abundance of the exploited red-rumped agouti (Dasyprocta leporina Rodentia: Dasyproctidae) on the island of Trinidad
  5. Biogeography
  6. The roles of possible geographic barriers and geological events on the phylogeographic structure of the Eastern broad toothed field mouse (Apodemus mystacinus)
  7. First record of Blanford’s Fox Vulpes cana (Blanford, 1877) from Iraq
  8. New records and southern range extension of the Annamite striped rabbit Nesolagus timminsi in Vietnam
  9. Ecology
  10. A pilot study of the use of dry dog food as an alternative attractant in mesocarnivore studies
  11. Seasonal diet composition of Himalayan goral (Naemorhedus goral) in Kajinag National Park, Jammu and Kashmir, India
  12. Seasonal and daily activity patterns of mammals in the colony of great cormorants
  13. First North African record of a melanistic common genet (Genetta genetta Linnaeus, 1758)
  14. Albinism in Brazilian common opossums (Didelphis aurita)
  15. Evolutionary biology
  16. Brain size evolution in small mammals: test of the expensive tissue hypothesis
  17. Taxonomy/phylogeny
  18. DNA barcoding of bats (Chiroptera) from the Colombian northern region
  19. Morphology and genetics concur that Anoura carishina is a synonym of Anoura latidens (Chiroptera, Glossophaginae)
  20. Taxonomy of Ctenomys (Rodentia: Ctenomyidae) in northwestern Patagonia, Argentina: the occurrence of the “mendocinus” lineage
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