Startseite Contribution of procoagulant phospholipids, thrombomodulin activity and thrombin generation assays as prognostic factors in intensive care patients with septic and non-septic organ failure
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Contribution of procoagulant phospholipids, thrombomodulin activity and thrombin generation assays as prognostic factors in intensive care patients with septic and non-septic organ failure

  • Patrick Van Dreden , Barry Woodhams , Aurélie Rousseau , Jean-François Dreyfus und Marc Vasse EMAIL logo
Veröffentlicht/Copyright: 19. August 2012
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Clinical Chemistry and Laboratory Medicine (CCLM)
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine (CCLM) Band 51 Heft 2

Abstract

Background: Multiple organ dysfunction syndrome (MODS) observed in patients with sepsis and in non-septic patients organ failure (OF) is associated with a high mortality rate. We investigated whether new coagulation assays [quantification of procoagulant phospholipids (PPL) activity, functional assays measuring the activity of thrombomodulin (TMa) or tissue factor (TFa) and thrombin generation using calibrated automated thrombography (CAT)] could constitute new tools to better understand the physiopathology of MODS and have any prognostic value.

Methods: We measured TMa, TFa, PPL and CAT in 32 healthy controls, 24 patients with sepsis and 26 patients with non-septic OF. We compared these parameters with usual coagulation assays [prothrombin time, activated partial thromboplastin time, protein C (PC), protein S, D-Dimers (D-Di), soluble thrombomodulin (sTM)] and markers of inflammation (IL-6, CRP). Samples were collected within 24 h of the diagnosis.

Results: TMa, TFa, PPL, the lag time and time to thrombin peak levels were increased in both groups of patients. For both groups D-Di, IL-6, CRP and endogenous thrombin potential (ETP) were higher in non-survivors than in survivors, while PC and PPL were lower in non-survivors than in survivors. TMa increase was more marked in non-survivors patients with OF, while the ratio TMa/sTM was low in non-survivors with sepsis. Received operating characteristic (ROC) curve analysis indicated that thrombin peak and ETP were the more powerful discriminating factors in patients with sepsis or non-septic OF, respectively.

Conclusions: PPL, TMa and CAT assays could represent promising tools to identify patients with increased risk of mortality in MODS and could procure insights into pathogenesis of MODS.

Keywords: calibrated automated thrombography; non-septic organ failure; procoagulant phospholipids; sepsis; thrombomodulin activity; tissue factor activity
Corresponding author: Marc Vasse, UF d’Hémostase Cellulaire, Laboratoire d’Hématologie, IBC-CHU de Rouen & 3829 (Groupe MERCI), 1 rue de Germont, 76031 Rouen cedex, France, Phone: +33 1 46252957, Fax: +33 1 46252422

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Received: 2012-04-26
Accepted: 2012-07-23
Published Online: 2012-08-19
Published in Print: 2013-02-01

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm-2012-0262
Schlagwörter für diesen Artikel
calibrated automated thrombography; non-septic organ failure; procoagulant phospholipids; sepsis; thrombomodulin activity; tissue factor activity

Artikel in diesem Heft

  1. Letters to the Editor
  2. Performance evaluation of three different immunoassays for detection of antibodies to hepatitis B core
  3. Serum homocysteine concentrations in Chinese children with autism
  4. Interchangeability of venous and capillary HbA1c results is affected by oxidative stress
  5. Interference of hemoglobin (Hb) N-Baltimore on measurement of HbA1c using the HA-8160 HPLC method
  6. First human isolate of Mycobacterium madagascariense in the sputum of a patient with tracheobronchitis
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  14. Masthead
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  18. Commercial immunoassays in biomarkers studies: researchers beware!1)
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Heruntergeladen am 16.11.2025 von https://www.degruyterbrill.com/document/doi/10.1515/cclm-2012-0262/html
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