Fucosylation and Galactosylation of IgG Heavy Chains Differ between Acute and Remission Phases of Juvenile Chronic Arthritis
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Mirna Flögel
Abstract
Oligosaccharide structures are attached to nearly all membrane and serum proteins, and their composition changes significantly in many diseases. We have analysed glycosylation of IgG heavy chains in 34 patients with juvenile chronic arthritis and 13 control individuals. IgG was purified from 0.7 ml of serum, separated by electrophoresis and transferred on to polyvinylidene difluoride (PVDF) membrane. Ricinus communis agglutinin (RCA I) and Bandeirea simplicifolia (BSA II) and Ulex europaeus (UEA I) lectins were used to measure galactose, N-acetylglucosamine and fucose, respectively. While there was no significant difference in average levels of galactose and N-acetylglucosamine, patients with juvenile chronic arthritis had 2.4 times more fucose attached to IgG heavy chains than control individuals. A different picture emerged when patients were divided into those with acute disease and those in remission. Patients in whom juvenile chronic arthritis was currently active had significantly lower levels of galactose than those in remission, in whom galactose levels were comparable to the control group. Fucose levels in both groups of patients were significantly higher than in the control group. These results show that whereas de-galactosylation is a good test to detect and measure the activity of juvenile chronic arthritis, increased fucosylation is a much more reliable measure for diagnosis of the disease itself.
Copyright © 1998 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Genetic Approach for Insulin-Independent Diabetes Mellitus in Clinical Practice
- An Enzymatic Method for the Determination of Free Fatty Acids in Serum/Plasma
- Specificity of Cardiac Troponins I and T in Renal Disease
- Blood and Tissue Findings in the Diagnosis of Mesenteric Ischaemia: An Experimental study
- Fucosylation and Galactosylation of IgG Heavy Chains Differ between Acute and Remission Phases of Juvenile Chronic Arthritis
- Analytical Evaluation of an Improved Procedure for Measuring Thyrotropin
- Erroneous Monocyte and Neutrophil Counts with the Sysmex NE-1500 in Patients Recovering from Bone Marrow Aplasia
- N-Benzoyl, L-Glutamic Acid as a Suitable Internal Standard for the Analysis of trans,trans-Muconic Acid in Human Urine by Liquid Chromatography
- Acute Exogenous Elevation of Plasma Free Fatty Acids Does Not Influence the Plasma Magnesium Concentration
- Characterisation and Evaluation of External Quality Assessment Scheme Serum
- Need for Correct Estimates of Biological Variation: The Example of C-Reactive Protein
