Analytical Evaluation of an Improved Procedure for Measuring Thyrotropin
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Bernardino González-de-la-Presa
Abstract
The analytical characteristics of the AxSYM Ultrasensitive hTSH-II (Abbott Laboratories) procedure for quantitation of serum thyrotropin (TSH) concentration were evaluated. Within- and between-run imprecisions, functional sensitivity, analytical interval and relative inaccuracy with respect to the Enzymum-Test TSH (Boehringer Mannheim) were studied. In all cases, the within-run and between-run of coefficients variation were lower than 6.69 % and 8.12 % respectively. The measurement range was tested with serial dilutions of a serum with a high thyrotropin concentration, and the procedure was found to be linear up to at least 87.0 mIU/l. The functional sensitivity was 0.018 mIU/l. The relative inaccuracy study (Passing-Bablok non-parametric linear regression) produced the following linear equation:
(AxSYM) = 1.02. (ES-700)−0.03 mIU/l, with 95 % confidence intervals of a(−0.05; −0.01); b(0.98; 1.06).
Copyright © 1998 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Genetic Approach for Insulin-Independent Diabetes Mellitus in Clinical Practice
- An Enzymatic Method for the Determination of Free Fatty Acids in Serum/Plasma
- Specificity of Cardiac Troponins I and T in Renal Disease
- Blood and Tissue Findings in the Diagnosis of Mesenteric Ischaemia: An Experimental study
- Fucosylation and Galactosylation of IgG Heavy Chains Differ between Acute and Remission Phases of Juvenile Chronic Arthritis
- Analytical Evaluation of an Improved Procedure for Measuring Thyrotropin
- Erroneous Monocyte and Neutrophil Counts with the Sysmex NE-1500 in Patients Recovering from Bone Marrow Aplasia
- N-Benzoyl, L-Glutamic Acid as a Suitable Internal Standard for the Analysis of trans,trans-Muconic Acid in Human Urine by Liquid Chromatography
- Acute Exogenous Elevation of Plasma Free Fatty Acids Does Not Influence the Plasma Magnesium Concentration
- Characterisation and Evaluation of External Quality Assessment Scheme Serum
- Need for Correct Estimates of Biological Variation: The Example of C-Reactive Protein
